Cytogenetics

细胞遗传学

基本信息

批准号：
7944991
负责人：
ROBERT B. JENKINS
金额：
$ 9.99万
依托单位：
MAYO CLINIC ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-04-17 至 2014-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7944991
关键词：
Animal Model Applications Grants Cancer Center Cancer Center Support Grant Cell Culture Techniques Cells Chromosome Breakage Clinical Clonality Color Comprehensive Cancer Center Cultured Cells Custom Cytogenetic Analysis Cytogenetics Cytogenetics Shared Resource Data Analyses Development Diagnostic Disease Embryo Fibroblasts Fluorescent in Situ Hybridization Freezing Genes Genetic Grant Hereditary Disease Home environment Human Hybrid Cells Image Interphase Laboratories Location Malignant Neoplasms Maps Mayo Clinic Cancer Center Metaphase Microscope Molecular Cytogenetics Monitor Mus Paraffin Embedding Pattern Process Production Prognostic Marker Publications Research Research Personnel Resource Sharing Sampling Scientist Services Slide Specimen Spectral Karyotyping System Techniques Tissue Microarray Tissues Transgenes Tumor Cell Line Visual Aid Xenograft procedure cell type established cell line fluorescence microscope meetings member nerve stem cell novel programs research study tumor

项目摘要

The central focus of the Cytogenetics Shared Resource (CSR) is to provide cytogenetic and molecular cytogenetic analysis of human and animal model research samples. These samples may be in the form of cultured cells, fresh or frozen tissue, paraffin-embedded sections, or tissue microarrays (TMAs). Specific cell types include tumor cell lines, xenograft cells, hybrid cells from chimeric animal models, neural stem cells, and mouse embryonic fibroblasts, among others. The CSR provides specialized services including cell culture, routine cytogenetic analysis, chromosome breakage analysis, interphase and metaphase Fluorescence in situ Hybridization (FISH), Spectral Karyotyping (SKY) analysis of human and mouse metaphases, and homebrew FISH Probe Production. This last service provides novel FISH probes that are specific to investigators needs and are usually not commercially available. These probes are then used for FISH analysis studies. The CSR is expanding this service beyond homebrew enumeration FISH probes by creating custom translocation/fusion probes, break-apart probes, and multi-color FISH probes to ultimately assist in developing strategic probes that become clinical diagnostic and prognostic markers of disease. Other services provided by the CSR to meet the needs of investigators include utilization of the CSR laboratory for slide processing and fluorescent microscope and imaging system use. Briefly, applications of CSR services include: to characterize the clonality of tumor specimens; to determine the origin of a specific tumor culture (i.e., mouse vs. human), to aid in the identification of genetic regions important for the development of specific malignancies, and to map the location of DMA sequences, genes, or transgene insertions. With the recognition that cancer is a genetic disease, many cancer researchers, including scientists in the Mayo Clinic Cancer Center (MCCC), require access to CSR services. This Shared Resource is utilized by 9 of the 12 Cancer Center Programs. Approximately 66% of CSR users are CCSG members and represent 92% of the overall utilization.

细胞遗传学共享资源（CSR）的主要重点是提供细胞遗传学和分子人类和动物模型研究样本的细胞遗传学分析。这些样本可能是培养的细胞，新鲜或冷冻的组织，石蜡包裹的切片或组织微阵列（TMA）。特定细胞类型包括肿瘤细胞系，异种移植细胞，来自嵌合动物模型的杂化细胞，神经干细胞，和小鼠胚胎成纤维细胞等。企业社会责任提供包括牢房在内的专业服务培养，常规的细胞遗传学分析，染色体破裂分析，中期和中期荧光原位杂交（FISH），人和小鼠的光谱核分型（天空）分析中期和自制鱼类探针生产。这项最后的服务提供了新颖的鱼类探针特定于研究人员的需求，通常不可商业地使用。然后将这些探针用于鱼类分析研究。企业社会责任正在将这项服务扩展到自制枚举的鱼类探针之外创建自定义转运/融合探针，突破性探针和多色鱼类探针最终协助开发成为疾病的临床诊断和预后标志的战略探针。 CSR提供的其他服务满足调查人员的需求，包括利用CSR 用于滑动处理以及荧光显微镜和成像系统使用的实验室。简而言之，申请 CSR服务包括：表征肿瘤标本的克隆性；确定特定的起源肿瘤培养（即小鼠与人），以帮助鉴定对遗传区域开发特定的恶性肿瘤，并绘制DMA序列，基因或转基因的位置插入。认识到癌症是一种遗传疾病，许多癌症研究人员，包括 Mayo临床癌症中心（MCCC）的科学家需要获得CSR服务。这个共享资源由12个癌症中心计划中的9个使用。大约66％的CSR用户是CCSG成员并占整体利用率的92％。