Cytogenetics

细胞遗传学

• 批准号: 7944991
• 负责人: ROBERT B. JENKINS
• 金额: $ 9.99万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-17 至 2014-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7944991
• 关键词: Animal Model; Applications Grants; Cancer Center; Cancer Center Support Grant; Cell Culture Techniques; Cells; Chromosome Breakage; Clinical; Clonality; Color; Comprehensive Cancer Center; Cultured Cells; Custom; Cytogenetic Analysis; Cytogenetics; Cytogenetics Shared Resource; Data Analyses; Development; Diagnostic; Disease; Embryo; Fibroblasts; Fluorescent in Situ Hybridization; Freezing; Genes; Genetic; Grant; Hereditary Disease; Home environment; Human; Hybrid Cells; Image; Interphase; Laboratories; Location; Malignant Neoplasms; Maps; Mayo Clinic Cancer Center; Metaphase; Microscope; Molecular Cytogenetics; Monitor; Mus; Paraffin Embedding; Pattern; Process; Production; Prognostic Marker; Publications; Research; Research Personnel; Resource Sharing; Sampling; Scientist; Services; Slide; Specimen; Spectral Karyotyping; System; Techniques; Tissue Microarray; Tissues; Transgenes; Tumor Cell Line; Visual Aid; Xenograft procedure; cell type; established cell line; fluorescence microscope; meetings; member; nerve stem cell; novel; programs; research study; tumor

The central focus of the Cytogenetics Shared Resource (CSR) is to provide cytogenetic and molecular cytogenetic analysis of human and animal model research samples. These samples may be in the form of cultured cells, fresh or frozen tissue, paraffin-embedded sections, or tissue microarrays (TMAs). Specific cell types include tumor cell lines, xenograft cells, hybrid cells from chimeric animal models, neural stem cells, and mouse embryonic fibroblasts, among others. The CSR provides specialized services including cell culture, routine cytogenetic analysis, chromosome breakage analysis, interphase and metaphase Fluorescence in situ Hybridization (FISH), Spectral Karyotyping (SKY) analysis of human and mouse metaphases, and homebrew FISH Probe Production. This last service provides novel FISH probes that are specific to investigators needs and are usually not commercially available. These probes are then used for FISH analysis studies. The CSR is expanding this service beyond homebrew enumeration FISH probes by creating custom translocation/fusion probes, break-apart probes, and multi-color FISH probes to ultimately assist in developing strategic probes that become clinical diagnostic and prognostic markers of disease. Other services provided by the CSR to meet the needs of investigators include utilization of the CSR laboratory for slide processing and fluorescent microscope and imaging system use. Briefly, applications of CSR services include: to characterize the clonality of tumor specimens; to determine the origin of a specific tumor culture (i.e., mouse vs. human), to aid in the identification of genetic regions important for the development of specific malignancies, and to map the location of DMA sequences, genes, or transgene insertions. With the recognition that cancer is a genetic disease, many cancer researchers, including scientists in the Mayo Clinic Cancer Center (MCCC), require access to CSR services. This Shared Resource is utilized by 9 of the 12 Cancer Center Programs. Approximately 66% of CSR users are CCSG members and represent 92% of the overall utilization.

细胞遗传学共享资源（CSR）的中心焦点是提供细胞遗传学和分子生物学的信息。 人类和动物模型研究样品的细胞遗传学分析。这些样品可以是 培养细胞、新鲜或冷冻组织、石蜡包埋切片或组织微阵列（TMA）。特定细胞 类型包括肿瘤细胞系、异种移植细胞、来自嵌合动物模型的杂交细胞，神经干细胞， 和小鼠胚胎成纤维细胞等。CSR提供专门的服务， 培养、常规细胞遗传学分析、染色体断裂分析、间期和中期 人和小鼠的荧光原位杂交（FISH）、光谱核型分析（SKY） 中期和自制FISH探针生产。最后一项服务提供了新颖的FISH探针， 专门针对研究者的需要，通常无法从市场上买到。这些探针用于 FISH分析研究。CSR正在通过以下方式将此服务扩展到自制枚举FISH探测器之外： 创建定制的易位/融合探针、分离探针和多色FISH探针， 协助开发成为疾病临床诊断和预后标志物的战略探针。 CSR为满足研究者需求而提供的其他服务包括使用CSR 用于玻片处理实验室和荧光显微镜及成像系统。简言之， CSR服务包括：表征肿瘤标本的克隆性;确定特定肿瘤的来源; 肿瘤培养（即，小鼠与人），以帮助鉴定对免疫系统重要的遗传区域。 特定恶性肿瘤的发展，并绘制DMA序列、基因或转基因的位置 插入。随着认识到癌症是一种遗传疾病，许多癌症研究人员，包括 马约诊所癌症中心（MCCC）的科学家需要获得CSR服务。此共享资源 被12个癌症中心项目中的9个使用。大约66%的CSR用户是CCSG成员 占总利用率的92%。