Cytogenetics

细胞遗传学

• 批准号: 7944991
• 负责人: ROBERT B. JENKINS
• 金额: $ 9.99万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-17 至 2014-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7944991
• 关键词: Animal Model; Applications Grants; Cancer Center; Cancer Center Support Grant; Cell Culture Techniques; Cells; Chromosome Breakage; Clinical; Clonality; Color; Comprehensive Cancer Center; Cultured Cells; Custom; Cytogenetic Analysis; Cytogenetics; Cytogenetics Shared Resource; Data Analyses; Development; Diagnostic; Disease; Embryo; Fibroblasts; Fluorescent in Situ Hybridization; Freezing; Genes; Genetic; Grant; Hereditary Disease; Home environment; Human; Hybrid Cells; Image; Interphase; Laboratories; Location; Malignant Neoplasms; Maps; Mayo Clinic Cancer Center; Metaphase; Microscope; Molecular Cytogenetics; Monitor; Mus; Paraffin Embedding; Pattern; Process; Production; Prognostic Marker; Publications; Research; Research Personnel; Resource Sharing; Sampling; Scientist; Services; Slide; Specimen; Spectral Karyotyping; System; Techniques; Tissue Microarray; Tissues; Transgenes; Tumor Cell Line; Visual Aid; Xenograft procedure; cell type; established cell line; fluorescence microscope; meetings; member; nerve stem cell; novel; programs; research study; tumor

The central focus of the Cytogenetics Shared Resource (CSR) is to provide cytogenetic and molecular cytogenetic analysis of human and animal model research samples. These samples may be in the form of cultured cells, fresh or frozen tissue, paraffin-embedded sections, or tissue microarrays (TMAs). Specific cell types include tumor cell lines, xenograft cells, hybrid cells from chimeric animal models, neural stem cells, and mouse embryonic fibroblasts, among others. The CSR provides specialized services including cell culture, routine cytogenetic analysis, chromosome breakage analysis, interphase and metaphase Fluorescence in situ Hybridization (FISH), Spectral Karyotyping (SKY) analysis of human and mouse metaphases, and homebrew FISH Probe Production. This last service provides novel FISH probes that are specific to investigators needs and are usually not commercially available. These probes are then used for FISH analysis studies. The CSR is expanding this service beyond homebrew enumeration FISH probes by creating custom translocation/fusion probes, break-apart probes, and multi-color FISH probes to ultimately assist in developing strategic probes that become clinical diagnostic and prognostic markers of disease. Other services provided by the CSR to meet the needs of investigators include utilization of the CSR laboratory for slide processing and fluorescent microscope and imaging system use. Briefly, applications of CSR services include: to characterize the clonality of tumor specimens; to determine the origin of a specific tumor culture (i.e., mouse vs. human), to aid in the identification of genetic regions important for the development of specific malignancies, and to map the location of DMA sequences, genes, or transgene insertions. With the recognition that cancer is a genetic disease, many cancer researchers, including scientists in the Mayo Clinic Cancer Center (MCCC), require access to CSR services. This Shared Resource is utilized by 9 of the 12 Cancer Center Programs. Approximately 66% of CSR users are CCSG members and represent 92% of the overall utilization.

细胞遗传学共享资源(CSR)的核心是提供细胞遗传学和分子生物学 人和动物模型研究样本的细胞遗传学分析。这些样本的形式可能是 培养细胞、新鲜或冰冻组织、石蜡包埋切片或组织微阵列(TMA)。特定细胞 类型包括肿瘤细胞系、异种移植细胞、来自嵌合动物模型的杂交细胞、神经干细胞、 以及小鼠胚胎成纤维细胞等。CSR提供包括CELL在内的专业服务 培养、常规细胞遗传学分析、染色体断裂分析、间期和中期 人和小鼠的荧光原位杂交和光谱核型分析 中期，和自制鱼探测生产。这最后一项服务提供了新的鱼类探测器， 专门针对调查人员的需要，通常不能在商业上获得。然后，这些探头被用于 鱼类分析研究。CSR正在通过以下方式将这项服务扩展到自制列举鱼类探测器之外 创建自定义易位/融合探针、分离探针和多色鱼类探针，以最终 协助开发成为疾病临床诊断和预后标记物的战略性探针。 企业社会责任为满足调查人员的需要而提供的其他服务包括利用企业社会责任 实验室用于玻片处理和荧光显微镜及成像系统。简言之，它的应用 CSR服务包括：表征肿瘤标本的克隆性；确定特定来源的 肿瘤培养(即小鼠与人)，以帮助识别对 特定恶性肿瘤的发展，并绘制DMA序列、基因或转基因的位置 插页。随着癌症是一种遗传病的认识，许多癌症研究人员，包括 梅奥诊所癌症中心(MCCC)的科学家需要获得企业社会责任服务。此共享资源 被12个癌症中心项目中的9个项目使用。大约66%的CSR用户是CCSG成员 占总利用率的92%。