CORE--CYTOGENETICS

随着癌症是一种遗传性疾病的认识，许多癌症研究人员，包括梅奥诊所癌症中心(MCCC)的科学家，需要获得常规和高级细胞遗传学服务。这些服务包括常规细胞遗传学分析、中期光谱核型(Sky)分析、间期和中期荧光原位杂交(FISH)、比较基因组杂交(CGH)以及预定的荧光显微镜和成像系统时间。这种服务可以帮助识别对特定恶性肿瘤的发展至关重要的基因区域。这种服务也可用于表征肿瘤标本的克隆性并确定特定的来源(例如，老鼠与人 肿瘤培养。在过去的5年资助期内，共享的资源支持了30多名癌症中心成员的研究，分别对100、70、30和900多个病例进行了常规细胞遗传学、CGH、Sky和FISH分析。共享资源计划增加比较基因组杂交到阵列(CGHa)服务。因此，细胞遗传学共享资源为MCCC成员提供常规细胞遗传学和高级分子细胞遗传学服务。

With the recognition that cancer is a genetic disease, many cancer researchers, including scientists in the Mayo Clinic Cancer Center (MCCC), require access to routine and advanced cytogenetics services. These services include routine cytogenetic analysis, spectral karyotype (SKY) analyses of metaphases, interphase and metaphase fluorescence in situ hybridization (FISH), comparative genomic hybridization (CGH), and schedulable fluorescent microscope and imaging system time. Such services can aid in the identification of genetic regions important for the development of specific malignancies. Such services can also be used to characterize the clonality of tumor specimens and determine the origin (e.g., mouse vs. human) of a specific tumor culture. The shared resource has supported the research of over 30 Cancer Center members during the past 5-year funding period, performing routine cytogenetic, CGH, SKY and FISH analyses on over 100,70, 30 and 900 cases, respectively. The shared resource plans to add comparative genomic hybridization to array (CGHa) services. Thus, the Cytogenetics Shared Resource provides routine cytogenetic and advanced molecular cytogenetic services to MCCC members.