CORE--CYTOGENETICS

核心--细胞遗传学

• 批准号: 6989951
• 负责人: ROBERT B. JENKINS
• 金额: $ 8.39万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-15 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6989951
• 关键词: biomedical facility; comparative genomic hybridization; cytogenetics; fluorescence microscopy; fluorescent in situ hybridization; karyotype; neoplasm /cancer; neoplasm /cancer genetics; oncology

With the recognition that cancer is a genetic disease, many cancer researchers, including scientists in the Mayo Clinic Cancer Center (MCCC), require access to routine and advanced cytogenetics services. These services include routine cytogenetic analysis, spectral karyotype (SKY) analyses of metaphases, interphase and metaphase fluorescence in situ hybridization (FISH), comparative genomic hybridization (CGH), and schedulable fluorescent microscope and imaging system time. Such services can aid in the identification of genetic regions important for the development of specific malignancies. Such services can also be used to characterize the clonality of tumor specimens and determine the origin (e.g., mouse vs. human) of a specific tumor culture. The shared resource has supported the research of over 30 Cancer Center members during the past 5-year funding period, performing routine cytogenetic, CGH, SKY and FISH analyses on over 100,70, 30 and 900 cases, respectively. The shared resource plans to add comparative genomic hybridization to array (CGHa) services. Thus, the Cytogenetics Shared Resource provides routine cytogenetic and advanced molecular cytogenetic services to MCCC members.

认识到癌症是一种遗传性疾病，许多癌症研究人员，包括马约诊所癌症中心（MCCC）的科学家，需要获得常规和先进的细胞遗传学服务。这些服务包括常规细胞遗传学分析、中期分裂相的光谱核型分析、间期和中期荧光原位杂交、比较基因组杂交以及可重复的荧光显微镜和成像系统时间。此类服务可以帮助识别对特定恶性肿瘤发展重要的遗传区域。这种服务还可以用于表征肿瘤标本的克隆性并确定起源（例如，小鼠与人）的特定 肿瘤培养在过去5年的资助期间，共享资源支持了30多个癌症中心成员的研究，分别对100、70、30和900多个病例进行了常规细胞遗传学、CGH、SKY和FISH分析。共享资源计划增加比较基因组杂交阵列（CGHa）服务。因此，细胞遗传学共享资源为MCCC成员提供常规的细胞遗传学和先进的分子细胞遗传学服务。