Transgenic
转基因
基本信息
- 批准号:7945054
- 负责人:
- 金额:$ 14.21万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2009
- 资助国家:美国
- 起止时间:2009-04-17 至 2014-02-28
- 项目状态:已结题
- 来源:
- 关键词:AllelesAnimal ModelAnimalsApplications GrantsArizonaAutologousBiological AssayBiopsyBreedingCell Culture TechniquesCell LineCell SeparationCell divisionCellsCharacteristicsChimera organismChromosomesClinicCloning VectorsCollectionComplementary DNAComprehensive Cancer CenterConsultationsDNADNA Sequence RearrangementDevelopmentDrug resistanceES Cell LineElectroporationEmbryoEmbryo TransferEventFemaleFloridaFosteringFreezingFrequenciesFundingGene ExpressionGene MutationGene SilencingGene TargetingGene Transfer TechniquesGenerationsGenesGenetically Engineered MouseGenomicsGerm LinesGrantHSV-Tk GeneHaploidyHeterozygoteHousingHumanHuman ResourcesHybridsInjection of therapeutic agentInterferon Type IIJournalsKaryotype determination procedureKnock-outKnockout MiceLaboratoriesLacZ GenesMalignant NeoplasmsMayo Clinic Cancer CenterMetaphase SpreadMicroinjectionsMolecular CloningMolecular GeneticsMolecular ProfilingMothersMouse StrainsMouse, Founder, TransgenicMusMutationNatureNeomycinNewborn InfantNursesPhasePlasmid Cloning VectorPlasmidsPlayPoint MutationPositioning AttributePreparationPrincipal InvestigatorProceduresProductionProtocols documentationPublicationsPuromycinRegulationResearchResearch PersonnelResource SharingRoleScheduleSeriesServicesShippingShipsSiteSourceSouthern BlottingStagingSuperovulationSurrogate MothersSystemTailTaq PolymeraseTestingTetracyclinesTissuesTransgenesTransgenic AnimalsTransgenic MiceTransgenic OrganismsVertebral columnViralWorkbaseblastocystcancer therapycell typecohortcostdesigneggembryonic stem cellexperiencefusion genehomologous recombinationhygromycin Ainterestknockout genemalemeetingsmembermouse modelmutantoffspringoverexpressionprogramspromoterpupresearch studystemsuccesstooltransgene expressiontransmission processvectorweb sitezygote
项目摘要
Genetically engineered mice serve as a powerful tool for studying the molecular genetic basis of human
cancer and for testing new cancer therapies. The main purpose of the Transgenic and Gene Targeted
Mouse Shared Resource (TGTMSR) is to generate genetically altered mice for all Mayo Clinic investigators.
The TGTMSR provides two main services, the generation of gene-targeted mice and the production of
transgenic animals. The gene targeting service was established in 1999, while the transgenic service is a
new service established in 2006 and included in this application for support. Services are very
comprehensive allowing investigators with little or no prior experience in generating transgenics or knockouts
to implement genetically altered mice into their research programs. We design and purify transgenic DNA
constructs and inject these into fertilized eggs. We generate transgenic founders from these eggs and cross
these mice to wildtype mice to transmit the transgene through the germline. Furthermore, we design gene
targeting vectors (to generate classical or conditional knockouts, hypomorphic alleles or to introduce point
mutations in endogenous genes), electroporate these into ES cells and screen for clones carrying the
desired alteration by southern blotting. Correctly targeted clones are karyotyped and injected into host
blastocysts. Chimeric males are bred to wildtype females to establish germline transmission of the targeted
mutation. Our success rate of generating transgenics is thus far 100%, while that of gene targeting is >95%.
All users over the current funding period have been Mayo Clinic Cancer Center (MCCC) members. Studies
using mouse models generated by the TGTMSR have led to a series of scientific publications over the past 5
years, several of which were in outstanding scientific journals. A total of 27 grant applications supported by
the TGTMSR were funded, 13 of which were from the NCI.
基因工程小鼠是研究人类遗传物质分子遗传基础的有力工具,
癌症和测试新的癌症疗法。转基因和基因打靶的主要目的
小鼠共享资源(TGTMSR)将为所有马约诊所的研究者生产基因改变的小鼠。
TGTMSR提供两项主要服务,即基因靶向小鼠的产生和
转基因动物基因靶向服务成立于1999年,而转基因服务是一个
新服务于2006年建立,并包含在此支持申请中。服务非常
全面的允许研究者在产生转基因或基因敲除方面几乎没有或没有经验
将转基因小鼠应用到他们的研究项目中。我们设计并纯化了转基因DNA
构建并将其注入受精卵。我们从这些卵子中产生转基因创始人,
将这些小鼠转化为野生型小鼠以通过种系传递转基因。此外,我们设计基因
靶向载体(以产生经典或条件性敲除、亚纯型等位基因或引入点
内源性基因中的突变),将这些电穿孔到ES细胞中,并筛选携带内源性基因突变的克隆。
通过Southern印迹进行所需的改变。对正确靶向的克隆进行核型分析并注射到宿主中
囊胚将嵌合雄性与野生型雌性交配以建立靶向嵌合体的生殖系传播。
突变到目前为止,我们产生转基因的成功率是100%,而基因打靶的成功率是> 95%。
在当前资助期间,所有用户都是马约诊所癌症中心(MCCC)的成员。研究
使用TGTMSR生成的小鼠模型,在过去5年中发表了一系列科学出版物。
多年来,其中一些是在优秀的科学期刊。本署共批出二十七项资助申请,
TGTMSR获得资助,其中13个来自NCI。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Jan M. van Deursen其他文献
Cellular senescence in renal ageing and disease
肾脏衰老和疾病中的细胞衰老
- DOI:
10.1038/nrneph.2016.183 - 发表时间:
2016-12-28 - 期刊:
- 影响因子:39.800
- 作者:
Ines Sturmlechner;Matej Durik;Cynthia J. Sieben;Darren J. Baker;Jan M. van Deursen - 通讯作者:
Jan M. van Deursen
Chronic social stress induces p16-mediated senescent cell accumulation in mice
慢性社会压力诱导小鼠中 p16 介导的衰老细胞积累
- DOI:
10.1038/s43587-024-00743-8 - 发表时间:
2024-11-11 - 期刊:
- 影响因子:19.400
- 作者:
Carey E. Lyons;Jean Pierre Pallais;Seth McGonigle;Rachel P. Mansk;Charles W. Collinge;Matthew J. Yousefzadeh;Darren J. Baker;Patricia R. Schrank;Jesse W. Williams;Laura J. Niedernhofer;Jan M. van Deursen;Maria Razzoli;Alessandro Bartolomucci - 通讯作者:
Alessandro Bartolomucci
Jan M. van Deursen的其他文献
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{{ truncateString('Jan M. van Deursen', 18)}}的其他基金
The role of senescent cells in late-life tumorigenesis
衰老细胞在晚年肿瘤发生中的作用
- 批准号:
8984872 - 财政年份:2013
- 资助金额:
$ 14.21万 - 项目类别:
The role of senescent cells in late-life tumorigenesis
衰老细胞在晚年肿瘤发生中的作用
- 批准号:
8601177 - 财政年份:2013
- 资助金额:
$ 14.21万 - 项目类别:
The role of senescent cells in late-life tumorigenesis
衰老细胞在晚年肿瘤发生中的作用
- 批准号:
8435619 - 财政年份:2013
- 资助金额:
$ 14.21万 - 项目类别:
The role of senescent cells in late-life tumorigenesis
衰老细胞在晚年肿瘤发生中的作用
- 批准号:
8780613 - 财政年份:2013
- 资助金额:
$ 14.21万 - 项目类别:
Role of PTEN in Chromosome Segregation and its Importance for Tumor Suppression
PTEN 在染色体分离中的作用及其对肿瘤抑制的重要性
- 批准号:
8340701 - 财政年份:2012
- 资助金额:
$ 14.21万 - 项目类别:
Role of PTEN in Chromosome Segregation and its Importance for Tumor Suppression
PTEN 在染色体分离中的作用及其对肿瘤抑制的重要性
- 批准号:
8862427 - 财政年份:2012
- 资助金额:
$ 14.21万 - 项目类别:
Role of PTEN in Chromosome Segregation and its Importance for Tumor Suppression
PTEN 在染色体分离中的作用及其对肿瘤抑制的重要性
- 批准号:
9079435 - 财政年份:2012
- 资助金额:
$ 14.21万 - 项目类别:
Role of PTEN in Chromosome Segregation and its Importance for Tumor Suppression
PTEN 在染色体分离中的作用及其对肿瘤抑制的重要性
- 批准号:
8515369 - 财政年份:2012
- 资助金额:
$ 14.21万 - 项目类别:
Bub 1 in Chromosomal Instability and Tumorigenesis
Bub 1 在染色体不稳定性和肿瘤发生中的作用
- 批准号:
8025993 - 财政年份:2007
- 资助金额:
$ 14.21万 - 项目类别:
BUB1 in Chromosomal Instability and Tumorigenesis
BUB1 在染色体不稳定性和肿瘤发生中的作用
- 批准号:
8295681 - 财政年份:2007
- 资助金额:
$ 14.21万 - 项目类别:
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