Epigenomics Core

表观基因组学核心的任务是协助研究人员进行专门的表观基因组学分析 并对这些大型数据集进行全面的数据分析。通过与其他核心的协作努力 在DRTC中，明确的药理、饮食、环境和遗传改变的影响是 以其对葡萄糖动态平衡、胰岛素作用和新陈代谢的影响为特征。的作用 相关组织的基因修饰，如神经元、肝细胞、骨骼肌、脂肪细胞、β 而与葡萄糖动态平衡相关的免疫细胞可以通过彻底的和 在啮齿动物模型中进行了明确的实验。可以在功能上确认候选目标的识别 或者通过产生特定的小鼠模型来反驳，并通过比较在人类种群中进行验证 在多个队列/群体中进行分析。虽然这是一个快速发展的领域，但广泛的专业知识 聚集在爱因斯坦表观基因组学中心，包括分子遗传学，生物信息学和 计算生物学专业知识允许表观基因组学核心为DRTC调查人员提供服务 与了解新陈代谢相关的各种专门的、高质量的方法和工具 疾病过程。为了实现这些目标，表观基因组学核心将：1)向研究人员提供 专业的高通量分子技术资源，包括微阵列和大规模并行 研究DNA甲基化和染色质组织的测序平台；2)提供广泛的质量 所有高通量分析的控制和保证信息，作为每次分析的一部分；3) 提供随时可用的专用系统管理员和程序员、关系数据库服务、 通过计算和统计表观基因组学资源和研究信息学提供高性能的计算资源和数据存储/备份系统；4)就用于所提出的特定生物学问题的最佳实验平台和方案向研究人员提供建议；5)通过每周讲习班和期刊俱乐部传播当前和新的技术发展信息，并促进研究人员的互动；6)在实验室对学生、博士后研究员、研究人员和技术人员进行表观基因组学方法和数据分析方面的培训。所有这些服务都提供给糖尿病研究的新手，以及从事糖尿病相关项目的研究人员，这些项目可以通过使用这一核心的专门知识和设施得到丰富和扩展。

The mission of the Epigenomics Core is to assist investigators in performing specialized epigenomics assays and comprehensive data analyses of these large datasets. Through collaborative efforts with the other Cores of the DRTC, the effects of defined pharmacological, dietary, environmental and genetic alterations are thoroughly characterized for their effects on glucose homeostasis, insulin action, and metabolism. The role of genetic modifications in relevant tissues, such as neurons, hepatocytes, skeletal muscle, adipocytes, beta and immune cells that are related to glucose homeostasis can be directly compared by thorough and definitive experimentation in rodent models. Identification of candidate targets can be functionally confirmed or refuted by the generation of specific mouse models and validated in human populations by comparative analyses in multiple cohorts/populations. Although this is a rapidly developing field, the broad expertise assembled at Einstein's Center for Epigenomics including molecular genetics, bioinformatics and computational biology expertise allows the Epigenomics Core to provide services to DRTC investigators with a wide range of specialized, high quality methodologies and tools relevant to understanding metabolic disease processes. To accomplish these goals, the Epigenomics Core will: 1) make available to investigators specialized high-throughput molecular technological resources including microarray and massively-parallel sequencing platforms to study DNA methylation and chromatin organization; 2) provide extensive quality controls and assurance information for all high-throughput assays, generated as part of each analysis; 3) provide readily available dedicated systems administrators and programmers, relational database services, high-performance computing resources and data storage/backup systems through the Computational and Statistical Epigenomics Resource and Research Informatics; 4) advise investigators regarding the best experimental platforms and protocols to use for the specific biological question being asked; 5) disseminate current and new technological development information and foster investigator Interactions through weekly workshops and journal clubs; and 6) provide laboratory training of students, postdoctoral fellows, investigators and technical staff in performing epigenomics methodologies and data analyses. All these services are available to investigators new to diabetes research, as well as to investigators working on diabetes-related projects that can be enriched and extended by the use of the expertise and facilities of this core.