Function of Zscan4 in ES cells and early embryos

Zscan4 在 ES 细胞和早期胚胎中的功能

• 批准号: 7964018
• 负责人: Minoru Ko
• 金额: $ 30.47万
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7964018
• 关键词: Aging; Candidate Disease Gene; Cell Lineage; Cells; Development; Down-Regulation; Embryo; Gene Activation; Genes; Genetic Transcription; Genome; Goals; Hour; Implant; Inner Cell Mass; Knock-out; Maintenance; Molecular Profiling; Morula; Mus; Names; Organ; Pattern; RNA Degradation; Replacement Therapy; Research Project Grants; Role; Staging; Stem cells; Time; Undifferentiated; Uterus; Work; base; blastocyst; embryo cell; embryonic stem cell; experience; pluripotency; preimplantation; research study; self-renewal

We have further characterized Zscan4 in mouse 2-cell embryos and in the subpopulation of mouse ES cells. We found that other 2-cell-specific genes are also highly expressed in Zscan4(+) cells. This indicates that the subpopulation of ES cells marked by Zscan4 expression shows some resemblance to 2-cell embryos, whereas the majority of ES cells show similarity to the Inner Cell Mass (ICM) cells in blastocysts. By carrying out cell lineage tracing experiments, we have found that, although Zscan4 is expressed only in 5% of ES cells in culture at a given time, essentially all the ES cells experience Zscan4-positive state by 9 passages. We are currently carrying out the Zscan4 knockdown and knockout experiments.

我们进一步在小鼠2-细胞胚胎和小鼠ES细胞亚群中鉴定了Zscan4。我们发现，其他2细胞特异性基因在Zscan4(+)细胞中也高表达。这表明，Zscan4标记的ES细胞亚群与2-细胞胚胎有一定的相似性，而大多数ES细胞与囊胚中的内细胞团(ICM)细胞相似。通过进行细胞谱系追踪实验，我们发现，虽然Zscan4在特定时间只在培养的5%的ES细胞中表达，但基本上所有的ES细胞在9代时都经历了Zscan4阳性状态。我们目前正在进行Zscan4基因敲除和敲除实验。