Cellular Basis Of Action Of Gastrointestinal Peptides/Growth factors

最近的研究表明，胃肠激素/生长因子可能通过刺激细胞内多个酪氨酸磷酸化(TyrP)信号通路来刺激细胞生长。然而，目前对许多胃肠激素/生长因子激活胃肠道组织中这些级联反应的能力知之甚少。我们的研究主要集中在两个方面，包括主要通过酪氨酸激酶的细胞内信号级联反应的研究和试图开发新的生长抑制药物的肿瘤生长研究。最近的研究表明，胃肠激素类似于生长因子，可能通过刺激细胞内多个酪氨酸磷酸化(TyrP)级联来刺激细胞生长/细胞信号转导。虽然这些级联反应已经用生长因子进行了广泛的研究，但对这一领域的胃肠激素却知之甚少。这些研究的目的是阐明这一领域，主要集中在以胰腺腺泡为模型的自然细胞系统中，研究胆囊收缩素受体的级联和蛙皮素受体的激活。有关PKD1和GAB1磷酸化的研究最近已经完成，在这一年里，关于胃肠激素和生长因子激活AKT的能力的详细研究也完成了。关于PKC theta激活的研究以抽象的形式报道，将于今年晚些时候完成。今年还研究了新型细胞毒性非甾体抗炎药改变肺癌细胞生长的能力。

RRecent studies show that gastrointestinal hormones/growth factors may stimulate cell growth by stimulating multiple intracellular tyrosine phosphorylation (TyrP) signaling cascades. However at present little is known about the ability of many gastrointestinal hormones/growth factors to activate these cascades in GI tissues. Our studies have been in two general areas, which include studies of intracellular signaling cascades primarily by tyrosine kinases and studies of tumoral growth attempting to develop novel agents for growth inhibition. Recent studies show that gastrointestinal hormones, similar to growth factors, may stimulate cell growth/cell signaling by stimulating multiple intracellular tyrosine phosphorylation (TyrP) cascades. Whereas these cascades have been extensively investigated with growth factors, little is known in this area with may gastrointestinal hormones. The goal of these studies is to clarify this area primarily concentrating on cholecystokinin receptor cascades and bombesin receptor activation using primarily pancreatic acini as a model natural cell system. Studies involving phosphorylation of PKD1 and GAB1 have been recently completed and during this year a detailed study of the ability of gastrointestinal hormones and growth factors to activate AKT was completed. Studies of PKC theta activation were reported in abstract form and will be completed later this year. The ability of novel cytotoxic sulfur NSAID to alter growth of lung cancer cells was also studied this year.