Biophysical Characterization Of Macromolecules

大分子的生物物理表征

• 批准号: 7967861
• 负责人: PETER SCHUCK
• 金额: $ 14.93万
• 依托单位: NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7967861
• 关键词: Adaptor Signaling Protein; Biological; Biosensing Techniques; Calmodulin; Circular Dichroism Spectroscopy; Differential Scanning Calorimetry; Enzymes; Extracellular Domain; Field Flow Fractionation; Glutamate Receptor; Goals; Laboratories; Membrane Proteins; Methodology; Monoclonal Antibodies; Peptides; Proteins; Research; Signal Transduction; Surface Plasmon Resonance; System; T-Cell Activation; Techniques; Titrations; Toll-like receptors; Tubulin; Viral Proteins; analytical ultracentrifugation; anthrax edema factor; experience; human RORA protein; light scattering; macromolecule; microcalorimetry; new technology; particle

Experimental techniques employed are analytical ultracentrifugation, static and dynamic light scattering, field flow fractionation, isothermal titration microcalorimetry, differential scanning calorimetry, circular dichroism spectroscopy, and surface plasmon resonance biosensing. Molecules studied in this project include MHC-like viral proteins, extracellular domains of glutamate receptors, adaptor proteins involved in the formation of signaling particles after T-cell activation, the enzyme ROR-alpha, the adaptor protein TIRAP of the Toll-like receptor and MyD88, the anthrax edema factor interaction with calmodulin and a monoclonal antibody, tubulin, and peptides derived from malarial surface proteins.

采用的实验技术是分析超离心，静态和动态光散射，场流分馏，等温滴定微量热法，差示扫描量热法，圆二色光谱，和表面等离子体共振生物传感。本项目研究的分子包括MHC样病毒蛋白、谷氨酸受体的胞外结构域、参与T细胞活化后信号颗粒形成的衔接蛋白、ROR-alpha酶、Toll样受体和MyD 88的衔接蛋白TIRAP、炭疽水肿因子与钙调蛋白和单克隆抗体的相互作用、微管蛋白和源自疟疾表面蛋白的肽。