Epigenetic Regulation of Adipogenesis

脂肪生成的表观遗传调控

• 批准号: 7967813
• 负责人: Kai Ge
• 金额: $ 26.23万
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7967813
• 关键词: Accounting; Acetylation; Biological Assay; Diabetes Mellitus; Disease; Enzymes; Epigenetic Process; Functional RNA; Gene Activation; Gene Expression; Gene Expression Regulation; Histone Acetylation; Histone H3; Histones; Lipodystrophy; Lysine; Methylation; Methyltransferase; Morbidity - disease rate; Non-Insulin-Dependent Diabetes Mellitus; Obesity; PPAR gamma; Phase; Play; Regulation; Repression; Risk Factors; Role; Site; chromatin remodeling; histone modification; lipid biosynthesis; mortality; promoter

Specific Aim 1: examine the change of histone modifications on PPARgamma promoter in the early phase of adipogenesis. By ChIP assays, we have observed robust change of multiple histone acetylation and methylation on PPARgamma promoter in the early phase of adipogenesis. Specific Aim 2: investigate the functional significance of the histone modification change on PPARgamma promoter by depleting the responsible enzymes. Specific Aim 3: investigate the roles of other epigenetic mechanisms, in particular chromatin remodeling and non-coding RNAs, in regulation of PPARgamma expression and adipogenesis.

具体目的1：检测脂肪形成早期PPARgamma启动子上组蛋白修饰的变化。通过ChIP检测，我们观察到在脂肪形成的早期阶段，PPARgamma启动子上的多个组蛋白乙酰化和甲基化的稳健变化。 具体目标2：通过去除相关酶，研究PPARgamma启动子上组蛋白修饰变化的功能意义。 具体目标3：研究其他表观遗传机制的作用，特别是染色质重塑和非编码RNA，在调节PPARgamma表达和脂肪形成。