Mouse model of infant acute megakaryocytic leukemia

婴儿急性巨核细胞白血病小鼠模型

• 批准号: 7563228
• 负责人: Glen D Raffel
• 金额: $ 13.44万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-01 至 2011-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7563228
• 关键词: Acute Megakaryocytic Leukemias; Address; Affect; Alleles; Cells; Child; Chimeric Proteins; Chromosomal translocation; Complement; Data; Development; Disease; Down Syndrome; Fibrinogen; Functional disorder; Gene Components; Gene Dosage; Gene Expression; Genes; Hematopoiesis; Hematopoietic; Human; In Vitro; Infant; Knock-in Mouse; Knock-out; Knockout Mice; Mediating; Megakaryocyte Proliferation; Megakaryocytes; Megakaryocytopoieses; Modeling; Molecular; Mus; Mutate; Pathogenesis; Pathway interactions; Pattern; Phenotype; Physiological; Physiology; Preparation; Property; Proteins; Reagent; Regulation; Role; Therapeutic Intervention; in vivo; insight; leukemia; leukemogenesis; mouse model; novel; overexpression; promoter; stoichiometry; transcription factor

DESCRIPTION (provided by applicant): The majority of infant acute megakaryocytic leukemias (AMKL) in non-Down Syndrome children have a unique chromosomal translocation, t(1;22). This translocation fuses two novel genes believed to be transcription factors, OTT (a.k.a. RBM15) and MAL (a.k.a. MKL1/MRTF-A/BSAC), and results in the expression of a chimeric protein. Homologous structural motifs within the fusion have been identified in proteins known to be involved in differentiation, proliferation and leukemogenesis. To elucidate the pathophysiology induced by the OTT-MAL fusion protein, mice have been generated using a knock-in approach to express OTT-MAL from the OTT locus. By expressing the fusion protein from the endogenous OTT promoter, the stoichiometry and expression pattern of OTT-MAL found in the human leukemia should be reproduced. Evolving data suggests subtle differences in gene dosage and regulation have a critical impact on leukemogenesis and normal megakaryopoiesis. Identification of the pathways by which OTT-MAL may induce leukemogenesis will require a more comprehensive understanding of the component genes, OTT and MAL as their hematopoietic roles are unknown. Deletion of OTT or MAL in mice will provide an invaluable reagent in defining the pathways dysregulated by OTT-MAL in terms of gene expression and physiology. The role of OTT-MAL in t(1;22) AMKL will be investigated using routine models to address the following questions: Specific Aim 1. What are the consequences of OTT-MAL expression in mice? Specific Aim 2. What is the in vivo physiologic role of OTT and which pathways of endogenous OTT are utilized by the OTT-MAL fusion protein? Specific Aim 3. What role does MAL function have in normal physiology and in OTT-MAL-mediated dysregulation? The establishment and analysis of a murine model of t(1;22) AMKL will provide new opportunities for the study of pathogenesis in AMKL, potential therapeutic interventions and fundamental insight into megakaryocyte development.

描述（由申请人提供）：大多数非唐氏综合征儿童中的婴儿急性巨核细胞白血病（AMKL）具有独特的染色体易位t（1;22）。这种易位融合了两个新的基因，据信是转录因子，OTT（又名OTT）。RBM15）和MAL（a.k.a. MKL 1/MRTF-A/BSAC），并导致嵌合蛋白的表达。在已知参与分化、增殖和白血病发生的蛋白质中已经鉴定出融合内的同源结构基序。为了阐明由OTT-MAL融合蛋白诱导的病理生理学，使用敲入方法从OTT基因座表达OTT-MAL来产生小鼠。通过表达来自内源性OTT启动子的融合蛋白，应当再现在人白血病中发现的OTT-MAL的化学计量和表达模式。不断发展的数据表明，基因剂量和调控的细微差异对白血病发生和正常的巨核细胞生成有重要影响。 鉴定OTT-MAL可能诱导白血病发生的途径需要更全面地了解组成基因OTT和MAL，因为它们的造血作用尚不清楚。小鼠中OTT或MAL的缺失将为确定OTT-MAL在基因表达和生理学方面失调的途径提供宝贵的试剂。将使用常规模型研究OTT-MAL在t（1;22）AMKL中的作用，以解决以下问题： 具体目标1. OTT-MAL在小鼠中表达的后果是什么？ 具体目标2。 OTT在体内的生理作用是什么，OTT-MAL融合蛋白利用内源性OTT的哪些途径？ 具体目标3。 MAL功能在正常生理和OTT-MAL介导的失调中起什么作用？ t（1;22）AMKL小鼠模型的建立和分析将为AMKL发病机制的研究、潜在的治疗干预和巨核细胞发育的基础性认识提供新的机会。