Time-resolved measurements of breath hydrogen sulfide

呼吸硫化氢的时间分辨测量

• 批准号: 7911928
• 负责人: Steven M Massick
• 金额: $ 10万
• 依托单位: SOUTHWEST SCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2011-08-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7911928
• 关键词: Alveolar; Area; Breath Tests; Carbon Dioxide; Cardiovascular system; Chronic; Clinical; Clinical Research; Colitis; Computer software; Computers; Crohn' s disease; Detection; Development; Devices; Diagnosis; Doctor of Philosophy; Electronics; Engineering; Ethane; Exhalation; Funding; Gases; Gastrointestinal Diseases; Grant; Hydrogen; Hydrogen Sulfide; Immune; Individual; Inflammatory; Inflammatory Bowel Diseases; Irritable Bowel Syndrome; Lead; Measurement; Measures; Medical Research; Methane; Methods; Monitor; Neurologic; Noise; Optical Methods; Optics; Oral cavity; Participant; Patients; Phase; Physiological; Population; Production; Questionnaires; Reaction Time; Reporting; Research; Role; Sampling; Science; Simulate; Small Business Innovation Research Grant; Spectrum Analysis; Symptoms; System; Techniques; Technology; Testing; Therapeutic; Time; United States National Institutes of Health; Universities; Work; base; commercial application; cost; experience; gastrointestinal; heterodyning; instrument; prototype; public health relevance; rapid technique; response; sensor; water vapor; wireless network

DESCRIPTION (provided by applicant): Hydrogen sulfide (H2S), once identified exclusively as a pernicious toxic gas, is now recognized as a key participant in a myriad of physiological functions. Activity occurs in a wide variety of systems including periodontal, cardiovascular, neurological, inflammatory, and gastrointestinal. We have selected one of these areas and propose new research for examining the role of H2S in gastrointestinal illness including colitis, irritable bowel syndrome, and Crohn's disease. Our planned approach focuses on the development of a rapid, optical method for measuring hydrogen sulfide concentrations in exhaled breath. We anticipate that, by the end of the Phase II grant, we will have demonstrated in a clinical setting a compact, fully-automated instrument that can report H2S real-time concentration profiles with fast response times within individual exhalations. Detection sensitivity will be 50 ppb or better. Fast response will allow the H2S mouth contributions to be resolved temporally for alveolar contributions. Our Overall Hypothesis is that a portable easy to use breath hydrogen sulfide monitor can be developed to diagnose and manage irritable bowel syndrome (IBS) and inflammatory bowel disease (IBD). Our Specific Aim is to evaluate breath collected from patients with IBS and IBD to healthy controls, and compare the results to validated instruments such as symptom questionnaires and standard hydrogen and methane breath analysis. The proposed exhaled H2S analysis method uses an optical spectroscopy technique call noise-immune, cavity-enhanced, optical heterodyne measurement spectroscopy (NICE-OHMS). The technique was invented about 15 years ago by John Hall and co-workers at NIST. It can achieve extraordinary detection sensitivity for a variety of gases, but has been considered too difficult for commercial applications. We recently simplified NICE-OHMS by using low-cost, low-power electronics developed for wireless networking. Reliable gas commercial analyzers can now be built. NICE-OHMS should be useful for breath analysis. It works well with small gas samples (~50 std. cc per exhalation) and has fast time response. Fully engineered analyzers will be about the size of a desktop computer. PUBLIC HEALTH RELEVANCE: Breath analysis has the potential to become a widely-used, rapid method for therapeutic monitoring. Our proposed project focuses on detection of hydrogen sulfide in breath as a way of monitoring and developing treatments for debilitating gastrointestinal diseases such as irritable bowel syndrome, inflammatory bowel disease and Crohn's disease.

描述（由申请人提供）：硫化氢（H2S）曾经被认为是一种有害的有毒气体，现在被认为是无数生理功能的关键参与者。活动发生在各种各样的系统，包括牙周，心血管，神经，炎症和胃肠道。我们选择了其中一个领域，并提出了新的研究，以检查H2S在胃肠道疾病中的作用，包括结肠炎、肠易激综合征和克罗恩病。我们计划的方法侧重于开发一种快速、光学的方法来测量呼出气体中的硫化氢浓度。我们预计，在第二阶段授权结束时，我们将在临床环境中展示一种紧凑的全自动仪器，该仪器可以在单个呼气中快速响应，实时报告H2S浓度曲线。检测灵敏度为50 ppb或更高。快速反应将允许H2S口腔贡献暂时解决肺泡贡献。我们的总体假设是，可以开发一种易于使用的便携式呼吸硫化氢监测仪来诊断和治疗肠易激综合征（IBS）和炎症性肠病（IBD）。我们的具体目的是评估从IBS和IBD患者收集的呼气与健康对照，并将结果与经过验证的工具（如症状问卷和标准氢气和甲烷呼气分析）进行比较。提出的呼出H2S分析方法使用一种称为噪声免疫，腔增强，光学外差测量光谱（NICE-OHMS）的光谱学技术。这项技术是大约15年前由NIST的约翰·霍尔和他的同事发明的。它可以对各种气体实现非凡的检测灵敏度，但一直被认为很难用于商业应用。我们最近通过使用为无线网络开发的低成本，低功耗电子设备简化了NICE-OHMS。现在可以建立可靠的气体商业分析仪。NICE-OHMS应该对呼吸分析有用。它适用于小气体样品（每次呼气~50标准立方厘米），并具有快速的时间响应。完全工程化的分析仪将和台式电脑差不多大。