Single Cell Micro-chamber Array analysis

描述(申请人提供)：细胞亚群的胞浆异常通常是病理的标志。大多数分析方法将只提供关于整个细胞群体的细胞质的信息，而不是单个细胞或细胞亚群的信息。为了克服这一点，我们建议开发单细胞微腔阵列(SiCMA)技术。这种方法是基于CHORI的Kuypers博士的实验室的初步研究，他使用微室阵列来报告胞液成分的随机分布。在第一阶段的SBIR中，我们的目标是证明这种方法的原理，并为E&M设备公司和CHORI公司共同努力的进一步发展奠定基础。拟议研究的成功完成将建立我们在复杂细胞群体中测量与细胞表面标记相关的细胞液成分的能力，并为这项技术的商业化提供基础。

DESCRIPTION (provided by applicant): Abnormalities in the cytosol of subpopulations of cells are often a hallmark of pathology. Most analysis methods will only provide information on the cytosol of cell populations as a whole, not for individual cells or subpopulations of cells. To overcome this, we propose to develop Single Cell Micro-chamber Array (SiCMA) technology. This approach is based on preliminary studies in the laboratory of Dr. Kuypers at CHORI to use microchamber arrays to report on the stochastic distribution of cytosolic components. In this Phase I SBIR we aim to prove the principle of this approach and set the stage for further development in a joint effort between E&M devices and CHORI. The successful completion of the proposed studies will establish our ability to measure cytosolic components correlated with cell surface markers in a complex cell population, and provide the basis for commercialization of this technology.