Genetic Dissection of Trisomy 21

21 三体的基因剖析

基本信息

  • 批准号:
    8066350
  • 负责人:
  • 金额:
    $ 43.22万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2008
  • 资助国家:
    美国
  • 起止时间:
    2008-04-01 至 2013-03-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Trisomy 21/Down syndrome (DS) is one of the most important human genetic diseases. It currently affects approximately 350,000 people in the United States and more than 2,000,000 people worldwide. It is the most frequent live-born human aneuploidy. It is the most common genetic cause of congenital heart disease and mental retardation. It is a leading cause of gastrointestinal anomalies and megakaryoblastic leukemia. It causes early-onset Alzheimer-type neurodegeneration in nearly every individual with DS. The prevailing hypothesis is that the DS phenotypes are caused by the dosage imbalances of the specific genes on human chromosome (HSA) 21, which is supported by mouse-based experimental results. Many DS phenotypes, including valvuloseptal defects, megakaryoblastic leukemia, and amyloid plaque neuropathology, are highly specific and rarely observed in other human chromosomal disorders, suggesting that these phenotypes are the consequences of the triplications of specific causative genes on HSA 21. However, the efforts to isolate these genes have not been successful due to lacking of an effective approach. In this application, we propose a new genetic analysis strategy for DS based largely on generating and analyzing mouse mutants carrying nested duplications and deletions in the HSA 21 syntenic regions. The strategy is capable of isolating the causative genes for DS phenotypes and predicted to be efficient for the following reasons: (1) We have established the first group of the mouse models of DS trisomic for all of the HSA 21 syntenic regions on mouse chromosomes 10, 16, and 17 and exhibiting DS phenotypes. (2) We have established the MICER resource which provides the ready-made targeting vectors for efficient chromosome engineering in any regions of the mouse genome. (3) The Sanger Institute has approved our request to complete the public effort in generating the knockout mice for all of the orthologs of the HSA 21 genes and has begun the process of mutating 54 of these genes. A major task of studying any human genetic diseases is genetic analysis of the disorders with the goal of isolating the causative genes for the disease phenotypes because subsequent studies on these genes may unravel the true mechanisms of the disorders which are otherwise unattainable. The aims of this discovery-driven proposal are in-depth characterization of DS-associated congenital cardiovascular malformations in our new mutant mice and genetic dissection of this phenotype to identify the critical genomic region(s) and ultimately the causative gene(s). The attainment of our objectives should unravel the entry points to the mechanistic details leading to congenital cardiovascular malformations in DS and may yield rare insights on cardiac development, which may lead to novel strategies for prevention, diagnosis, and treatment of congenital heart disease in children and adults regardless of their states of ploidy. The genetic analysis strategy refined and new mouse mutants generated through this study will have a lasting impact on DS research and will particularly benefit the efforts to isolate causative genes for other major phenotypes of DS.
描述(申请人提供):21三体/唐氏综合征(DS)是人类最重要的遗传病之一。目前,它影响着美国约35万人和全球200多万人。这是最常见的活生生的人类非整倍体。它是导致先天性心脏病和智力低下的最常见的遗传原因。它是胃肠道异常和巨核细胞白血病的主要原因。它会导致几乎每一个DS患者的早发性阿尔茨海默型神经变性。流行的假说认为DS表型是由人类染色体(HSA)21上特定基因的剂量不平衡引起的,这一假设得到了基于小鼠的实验结果的支持。许多DS表型,包括瓣膜间隔缺陷、巨核细胞白血病和淀粉样斑块神经病理,都具有高度的特异性,在其他人类染色体疾病中很少观察到,这表明这些表型是特定致病基因在HSA21上三倍体的结果。然而,由于缺乏有效的方法,分离这些基因的努力并不成功。在这一应用中,我们提出了一种新的DS遗传分析策略,主要基于产生和分析携带HSA 21共线区域嵌套复制和缺失的小鼠突变体。该策略能够分离DS表型的致病基因,并被预测是有效的,原因如下:(1)我们已经建立了第一组小鼠的DS三体模型,包括小鼠染色体10、16和17上的所有HSA 21同线区域,并表现出DS表型。(2)我们已经建立了MICER资源,为在小鼠基因组的任何区域进行有效的染色体工程提供了现成的靶向载体。(3)桑格研究所已经批准了我们的请求,即完成公众努力,为所有HSA 21基因的同源基因产生基因敲除小鼠,并已开始突变其中54个基因的过程。研究人类遗传性疾病的一个主要任务是对这些疾病进行遗传分析,目的是分离出疾病表型的致病基因,因为对这些基因的后续研究可能会揭开这些疾病的真实机制,否则这些机制是无法实现的。这项以发现为导向的建议的目的是深入描述我们的新突变小鼠中DS相关的先天性心血管畸形的特征,并对这一表型进行遗传解剖,以确定关键基因组区域(S),并最终确定致病基因(S)。我们的目标的实现将揭开导致DS先天性心血管畸形的机制细节的切入点,并可能对心脏发育产生罕见的见解,这可能导致预防、诊断和治疗儿童和成人先天性心脏病的新策略,无论他们的倍性状态如何。通过这项研究改进的遗传分析策略和产生的新的小鼠突变将对DS的研究产生持久的影响,并将特别有利于分离DS其他主要表型的致病基因。

项目成果

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Eugene Yu其他文献

Eugene Yu的其他文献

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{{ truncateString('Eugene Yu', 18)}}的其他基金

CRISPR-mediated engineering and pilot study of mouse mutants of the bitter taste receptor genes
CRISPR介导的小鼠苦味受体基因突变体工程和初步研究
  • 批准号:
    10451169
  • 财政年份:
    2022
  • 资助金额:
    $ 43.22万
  • 项目类别:
Generation and analysis of new mouse models to determine novel therapeutic targets for Down syndrome-associated cognitive deficits
生成并分析新的小鼠模型以确定唐氏综合症相关认知缺陷的新治疗靶点
  • 批准号:
    10704099
  • 财政年份:
    2022
  • 资助金额:
    $ 43.22万
  • 项目类别:
Generation and analysis of new mouse models to determine novel therapeutic targets for Down syndrome-associated cognitive deficits
生成并分析新的小鼠模型以确定唐氏综合症相关认知缺陷的新治疗靶点
  • 批准号:
    10518886
  • 财政年份:
    2022
  • 资助金额:
    $ 43.22万
  • 项目类别:
Generation and analysis of new mouse models to determine novel therapeutic targets for Down syndrome-associated cognitive deficits
生成并分析新的小鼠模型以确定唐氏综合症相关认知缺陷的新治疗靶点
  • 批准号:
    10711887
  • 财政年份:
    2022
  • 资助金额:
    $ 43.22万
  • 项目类别:
Mutational analysis to understand the role of CHML in developmental regression
突变分析以了解 CHML 在发育回归中的作用
  • 批准号:
    8877784
  • 财政年份:
    2015
  • 资助金额:
    $ 43.22万
  • 项目类别:
Mutational analysis to understand the role of CHML in developmental regression
突变分析以了解 CHML 在发育回归中的作用
  • 批准号:
    9069905
  • 财政年份:
    2015
  • 资助金额:
    $ 43.22万
  • 项目类别:
Genetic Dissection of Trisomy 21
21 三体的基因剖析
  • 批准号:
    7585303
  • 财政年份:
    2008
  • 资助金额:
    $ 43.22万
  • 项目类别:
Genetic Dissection of Trisomy 21
21 三体的基因剖析
  • 批准号:
    8249053
  • 财政年份:
    2008
  • 资助金额:
    $ 43.22万
  • 项目类别:
Genetic Dissection of Trisomy 21
21 三体的基因剖析
  • 批准号:
    7781344
  • 财政年份:
    2008
  • 资助金额:
    $ 43.22万
  • 项目类别:
Gene Targeting & Transgenic Shared Resource
基因打靶
  • 批准号:
    10641712
  • 财政年份:
    1997
  • 资助金额:
    $ 43.22万
  • 项目类别:

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