Typhoon Imaging System for Molecular Nutrition Research

描述（由申请人提供）：申请资金从GE医疗生物科学公司购买台风9400成像系统，用于整合到营养科学跨部门研究生项目（IGPNS）共享仪器资源中。威斯康星大学麦迪逊分校的IGPNS包括来自16个不同部门（营养科学，生物化学，遗传学，生理学，医学等）的40多名教师培训师。IGPNS被公认为全球顶级营养科学培训计划之一，是分子营养科学领域的首要计划。该项目由NIH T32 （2T32-DK007665-16）培训资助资助，并已持续多年。为了保持和提高我们在这一领域的领导地位，并继续培养最有前途的年轻科学家，我们必须能够使用我们研究所依赖的最先进的仪器和设施。像台风9400这样的成像系统已经成为分子生物学中必不可少的研究工具，无论研究的是特定的实验生物还是特定的问题。该仪器可用于对各种分子生物学技术产生的样品进行极其敏感和定量的分析，包括DNA/RNA分析（EMSA, Northern blots， RNase和S1核酸酶保护试验等）和蛋白质分析（免疫印迹，35S代谢标记和脉冲追踪实验，1D和2D PAGE后的蛋白质染色等）。以前，我们很大程度上依赖于其他方法（例如，基于胶片的放射自显影术），这些方法远不如成像仪扫描，而且成本更高。台风9400成像仪将满足这一巨大需求。7个主要用户代表整个IGPNS提交了该提案。这7个项目中有6个目前得到了NIH的资助。公共卫生相关性：对主要用户正在进行的研究正在对营养的分子方面产生重要见解，因为它们与人类健康和疾病有关。这些问题包括矿物质代谢和体内平衡、营养状态的生物标志物、酒精代谢及其对产前和新生儿发育的影响、胃肠道生理和功能、脂质代谢的调节及其与肥胖和糖尿病的关系。我们也会从IGPNS内部吸引新用户；因此，一个可访问的成像仪将扩大和加强研究人员的研究项目，而不是列出的申请人。IGPNS的所有教师都从事对人类健康具有重要意义的研究，因此对NIH的目标也很重要。

DESCRIPTION (provided by applicant): Funds are requested to purchase a Typhoon 9400 Imaging System from GE Healthcare Biosciences Corporation for integration into the Interdepartmental Graduate Program in Nutritional Sciences (IGPNS) shared instrumentation resource. The IGPNS at the University of Wisconsin-Madison encompasses more than 40 faculty trainers from 16 different departments (Nutritional Sciences, Biochemistry, Genetics, Physiology, Medicine, etc.). The IGPNS is recognized as one of the top nutritional sciences training programs worldwide and is a premier program in the area of molecular nutritional sciences. This program is supported, and has been for many years, by an NIH T32 (2T32-DK007665-16) training grant. To maintain and advance our leadership in this field and to continue to train the most promising young scientists, it is essential that we have access to the state-of-the-art instrumentation and facilities on which our research depends. Imaging systems like the Typhoon 9400 have become essential research tools in molecular biology regardless of the particular experimental organism or particular problem under investigation. This instrument can be used to achieve extremely sensitive and quantitative analysis of samples generated by a wide variety of molecular biology techniques including DNA/RNA analysis (EMSA, Northern blots, RNase and S1 nuclease protection assays, etc) and protein analysis (immunoblotting, 35S metabolic labeling and pulse-chase experiments, protein staining following 1D and 2D PAGE, etc). Previously, we have largely relied on other methods (e.g., film-based autoradiography) that are far inferior to imager scanning and much more costly. The Typhoon 9400 Imager will fill this great need. Seven major users are submitting this proposal on behalf of the entire IGPNS. Six of these seven have current NIH funding. Public Health Relevance: The ongoing studies of the major users are yielding important insights into molecular aspects of nutrition as they relate to human health and disease. These include issues of mineral metabolism and homeostasis, biomarkers of nutrient status, alcohol metabolism and its effects on prenatal and neonatal development, gastrointestinal physiology and function, and the regulation of lipid metabolism and its relationship to obesity and diabetes. We will also attract new users from within the IGPNS; so an accessible imager will broaden and strengthen the research programs of researchers beyond the listed applicants. All IGPNS faculty are engaged in research of great significance to human health and therefore of importance to the aims of NIH.