Typhoon Variable Mode Imager

台风可变模式成像仪

• 批准号: 7793746
• 负责人: M. NEAL WAXHAM
• 金额: $ 17.86万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-27 至 2011-09-26
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793746
• 关键词: Agreement; Am 80; Anatomy; Arts; Beds; Brain; Brain imaging; Color; Commit; Computer software; Data; Data Collection; Fluorescence; Fluorescent Probes; Funding; Health Sciences; Individual; Left; Licensing; Maintenance; Modeling; Neurobiology; Research; Research Personnel; Research Support; Resolution; Scanning; Source; Staining method; Stains; Texas; Time; United States National Institutes of Health; Universities; Work; cohort; cost; design; flexibility; instrument; medical schools; operation; programs

DESCRIPTION (provided by applicant): We are requesting funds to purchase a Typhoon model 9410 multi-mode flat bed scanner that will support the research programs of eight NIH-funded investigators at the University of Texas Medical School at Houston. The total estimated usage from these eight investigators is 80%, leaving an additional 20% of instrument time to other investigators at the Medical School. This state-of-the-art scanner has the capabilities of supporting chemiluminescent, phosphorimaging and multi-color fluorescence applications required to accomplish the Specific Aims of each investigator described in the application. No other instrument in the Medical School with the capabilities of the Typhoon 9410 scanner is available. The model 9410, as configured, will have three excitations sources providing four excitation wavelengths and dichroic/emission filter combinations capable of resolving up to seven emission wavelengths. These features will provide investigators with excellent flexibility in the choice of fluorescent probes for their specific analyses. In addition, the 9410 has scanning capabilities to 10 <m/pixel resolution adequate for producing images of the brain-wide distribution of single and multi- colored immunofluorescent stained brain sections. The 10 <m scanning resolution will also provide support for the preliminary scanning of microarrays before more expensive and time consuming analyses are pursued. We are also requesting funds to purchase the DeCyder Differential Analysis software package for analyzing 2D DIGE data. The Typhoon and the DeCyder software package are designed to work seamlessly together for 2D DIGE analyses. Funds are also requested to support five network licenses for the ImageQuant analysis software. These licenses will enable up to five investigators to analyze simultaneously data on a workstation of their choice. This capability will significantly increase access to the scanner by freeing up the workstation that directly interfaces with data collection. Institutional support is significant. The UT Health Science Center has committed to support 50% of the maintenance agreement for this scanner and the Department of Neurobiology and Anatomy at the Medical School has agreed to support the other 50% of the maintenance costs. Therefore, the instrument will be fully supported at no cost to the individual research programs. In addition, the Department of Neurobiology and Anatomy has committed the space and technical staff support required to properly administer the operation of the instrument. In total, we have a cohort of well-funded NIH investigators without direct access to a multi-mode flat-bed scanning instrument. The requested Typhoon 9410 scanner will enormously facilitate the pace of their research programs and those of other investigators at the University of Texas Medical School in Houston.

描述（由申请人提供）：我们正在申请资金购买Typhoon 9410型多模式平板扫描仪，该扫描仪将支持休斯顿德克萨斯大学医学院八名NIH资助的研究人员的研究计划。这8名研究者的总使用率估计为80%，将另外20%的仪器时间留给医学院的其他研究者。这种最先进的扫描仪具有支持荧光、磷光成像和多色荧光应用的能力，这些应用是实现应用中描述的每个研究者的特定目标所必需的。医学院没有其他仪器具有Typhoon 9410扫描仪的功能。如所配置的，9410型将具有提供四个激发波长的三个激发源和能够分辨多达七个发射波长的二向色/发射滤波器组合。这些功能将为研究人员提供出色的灵活性，在选择荧光探针进行特定的分析。此外，9410具有10 μ m/像素分辨率的扫描能力，足以生成单色和多色免疫荧光染色脑切片的全脑分布图像。10 μ m的扫描分辨率还将为进行更昂贵和耗时的分析之前的微阵列初步扫描提供支持。我们还申请资金购买DeCyder差分分析软件包，用于分析2D DIGE数据。Typhoon和DeCyder软件包旨在无缝配合进行2D DIGE分析。还要求提供资金，以支持ImageQuant分析软件的五个网络许可证。这些许可证将使最多五名研究人员能够在他们选择的工作站上同时分析数据。这一能力将释放直接与数据收集接口的工作站，从而大大增加对扫描仪的访问。机构支持很重要。UT健康科学中心承诺支持该扫描仪50%的维护协议，医学院神经生物学和解剖学系同意支持其他50%的维护费用。因此，该仪器将得到充分支持，不对个别研究方案产生任何费用。此外，神经生物学和解剖学系已承诺提供适当管理仪器操作所需的空间和技术人员支持。总的来说，我们有一批资金充足的NIH研究人员，他们没有直接使用多模式平板扫描仪。所要求的台风9410扫描仪将极大地促进他们的研究计划的步伐，以及休斯顿德克萨斯大学医学院的其他研究人员的研究计划。