TRACKING OF SINGLE FLUORESCENTLY TAGGED CAMKII

单个荧光标记的 CAMKII 的追踪

• 批准号: 7600962
• 负责人: M. NEAL WAXHAM
• 金额: $ 0.14万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7600962
• 关键词: AMPA Receptors; Back; Calcium; Calmodulin; Complex; Computer Retrieval of Information on Scientific Projects Database; Dendritic Spines; Diffusion; Funding; Grant; Holoenzymes; Institution; Label; Monitor; Movement; Pathway interactions; Phosphotransferases; Play; Proteins; Research; Research Personnel; Resources; Role; Source; Synapses; Synaptic Transmission; Synaptic plasticity; Thinking; United States National Institutes of Health; calmodulin-dependent protein kinase II; density; postsynaptic; radius bone structure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ca2+/calmodulin-dependent protein kinase II (CaMKII) is a multimeric complex composed predominantly of two subunits, alpha and beta. This holoenzyme, with a radius of gyration of ~20nm, consists of twelve subunits and plays an important role in synaptic plasticity. During synaptic activation calcium enters dendritic spines where it loads onto calmodulin (CaM) that subsequently activates CaMKII. The activated kinase moves to the postsynaptic density, where it is thought to phosphorylate AMPA receptors, thus potentiating synaptic transmission. These qualitative observations have yet to be backed-up through quantitative analysis of kinase movement. We propose to monitor the traceability of fluorescently labeled CaMKII, with twelve fluorescent proteins (eGFP) attached, would allow us to quantify the effect of diffusion during this activation pathway.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 Ca2+/钙调蛋白依赖性蛋白激酶 II (CaMKII) 是一种多聚体复合物，主要由 α 和 β 两个亚基组成。这种全酶的回转半径约为 20 nm，由 12 个亚基组成，在突触可塑性中发挥着重要作用。在突触激活过程中，钙进入树突棘，加载到钙调蛋白 (CaM) 上，随后激活 CaMKII。激活的激酶移动到突触后密度，据信它会磷酸化 AMPA 受体，从而增强突触传递。这些定性观察结果尚未通过激酶运动的定量分析得到支持。 我们建议监测附有 12 种荧光蛋白 (eGFP) 的荧光标记 CaMKII 的可追溯性，这将使我们能够量化该激活途径中扩散的影响。