A Combined 600 MHz NMR Console for Studies of Cell Extracts and Biological Solids

用于研究细胞提取物和生物固体的组合 600 MHz NMR 控制台

• 批准号: 7791773
• 负责人: John D Gross
• 金额: $ 48.87万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-13 至 2013-03-12
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7791773
• 关键词: Area; Arts; Biological; Biological Markers; California; Cell Extracts; Detection; Development; Diagnostic; Health; Human; Investigation; Ligand Binding; Liquid substance; Magic; Metabolic Pathway; Molecular; Monitor; Neurodegenerative Disorders; Nuclear; Prions; Request for Applications; Resolution; Solid; Techniques; Translational Research; United States National Institutes of Health; Universities; base; cancer therapy; drug discovery; human disease; insight; instrument; macromolecule; research study; solid state; solid state nuclear magnetic resonance; tool

DESCRIPTION (provided by applicant): This application requests state-of-the-art components essential for 1) establishing biological solid-state NMR at UCSF; and 2) considerably enhancing current efforts focused on investigating metabolic pathways and biomarkers for human diseases. The needed instrument is a combined liquids and solid-state 600 MHz NMR spectrometer console and accessories that allow: 1) High-resolution triple-resonance magic-angle spinning experiments to perform conformational analyses of mammalian and fungal prion protein fibrils; 2) high-throughput, automated, multinuclear detection of cell extracts to establish correlations with NMR detectable biomarkers; and 3) development of new, ultra-sensitive NMR techniques to monitor ligand binding to macromolecules using Dynamic Nuclear Polarization. Each of these areas are heavily supported at the University of California by the NIH, with investigations spanning from basic to translational science. The new instrument will have a broad impact on human health by providing insight into molecular bases for neurodegenerative disease, diagnostic treatments for cancer, and new tools for drug discovery.

描述（由申请人提供）：该申请需要最先进的组件，这些组件对于1）在加州大学旧金山分校建立生物固态核磁共振至关重要;和2）大大加强当前专注于研究人类疾病代谢途径和生物标志物的努力。所需的仪器是组合的液体和固态600 MHz NMR光谱仪控制台和附件，其允许：1）高分辨率三重共振魔角旋转实验，以执行哺乳动物和真菌朊病毒蛋白原纤维的构象分析; 2）细胞提取物的高通量、自动化、多核检测，以建立与NMR可检测生物标志物的相关性;和3）开发新的、超灵敏的NMR技术，以使用动态核极化来监测配体与大分子的结合。这些领域中的每一个都得到了美国国立卫生研究院（NIH）在加州大学的大力支持，研究范围从基础科学到转化科学。新仪器将通过深入了解神经退行性疾病的分子基础、癌症的诊断治疗和药物发现的新工具，对人类健康产生广泛影响。

项目成果

Cleavage of talin by calpain promotes platelet-mediated fibrin clot contraction.

钙蛋白酶对塔林的切割促进了血小板介导的纤维蛋白血块收缩。

• DOI: 10.1182/bloodadvances.2021004582
• 发表时间: 2021-12-14
• 期刊: BLOOD ADVANCES
• 影响因子: 7.5
• 作者: Fong, Karen P.;Molnar, Kathleen S.;Agard, Nicholas;Litvinov, Rustem, I;Kim, Oleg, V;Wells, James A.;Weisel, John W.;DeGrado, William F.;Bennett, Joel S.
• 通讯作者: Bennett, Joel S.