Glycomics Equipment: NanoLC CHIP-QTOF Mass Spectrometer

描述（由申请人提供）：提议的仪器将成为几个NIH项目中集中于感染和疾病诊断的糖组学分析的中心分析工具。该仪器包括一个集成的纳米流液相色谱装置和一个四极杆飞行时间（Q-TOF）质量分析仪。该仪器与主要研究者实验室借来的现有仪器类似。新仪器将包括串联质谱能力，用于在线结构分析。该仪器将用于分析低聚糖，并确定其结构在高度复杂的生物混合物。该仪器将用于的项目包括：(1)胃粘蛋白中o -聚糖的测定，确定与幽门螺杆菌的相互作用位点；血清聚糖生物标志物早期检测幽门螺杆菌相关胃癌（Jay Solnick博士，医学院），(2)益生元和益生菌在坏死性小肠结肠炎中的作用（Mark Underwood博士，医学院），(3)纳米olc芯片/Q-TOF质谱分析低聚糖（Carlito Lebrilla博士，医学院化学系），(4)细胞裂解物和“hit化合物”分子复合物的质谱鉴定（物理系朱向东博士），(5)疾病聚糖标记物的寻找（医学院林洁博士），(6)多酶反应产物和中间体的分析（化学系陈希博士），(7)化疗药物的设计和合成（化学系杰奎琳·格维-黑格博士）。

DESCRIPTION (provided by applicant): The proposed instrument will be the central analytical tool for the glycomics profiling in several NIH projects focused on infection and disease diagnosis. The instrument includes an integrated nanoflow liquid chromatography device with a quadrupole time-of-flight (Q-TOF) mass analyzer. The instrument is similar to an existing instrument that is on loan in the Principal Investigator's laboratory. The new instrument will include tandem MS capability for online structural analysis. The proposed instrument will be used to analyze oligosaccharides and determine their structures in highly complicated biological mixtures. Projects that the instrument will be used for include: (1) The determination of O-glycans in stomach mucin to determine the site of interaction with Helicobacter pylori; Serum glycan biomarkers for early detection of H. pylori-associated gastric cancer (Dr. Jay Solnick, School of Medicine), (2) The effect of prebiotics and probiotics in necrotizing enterocolitis (Dr. Mark Underwood, School of Medicine), (3) The profiling of oligosaccharides by nanoLC Chip/Q-TOF mass spectrometry (Dr. Carlito Lebrilla, Department of Chemistry and School of Medicine), (4) Mass spectrometry identification of molecular complexes in cell lysates and "hit compounds" (Dr. Xiangdong Zhu, Department of Physics), (5) The search of glycans markers for disease (Dr. Kit Lam, School of Medicine), (6) The analysis of products and intermediates in multiple-enzyme reactions (Dr. Xi Chen, Department of Chemistry), and (7) The design and synthesis of chemotherapeutics (Dr. Jacqueline Gervay-Hague, Department of Chemistry). PUBLIC HEALTH RELEVANCE: A nanoflow liquid chromatography time-of-flight mass spectrometry instrument with an integrated microchip separation device is requested for performing glycomics profiling of biological fluids. The instrument will be used for research related to human health in terms of gut health, cancer diagnosis, and infection.