Biophysics Cell Membrane Probe

生物物理细胞膜探针

基本信息

批准号：
7794146
负责人：
WILLIAM E BROWNELL
金额：
$ 35.23万
依托单位：
BAYLOR COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-07-20 至 2011-07-19
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): A Biophysics Cell Membrane Probe (BCMProbe) is requested to expand biophysical studies on the membranes of living cells. The BCMProbe consists of optical tweezers integrated with an electrophysiology patch-clamp module and a standing wave microscope. The BCMProbe is a novel, unique instrument that can be used to modulate membrane mechanics as well as the magnitude of the electric field across it. At the same time it can measure the force produced by the membrane and the membrane current. Major users will exploit the instrument to further examine membrane electromechanics and actin bundling in nanoscale membrane tubes, measure charge movement associated with cell deformation and examine the self association as well as the mobility of integral membrane proteins. We will be able to expand our previous work that has demonstrated cell membranes capable of converting electrical energy directly into mechanical energy at acoustic frequencies. In addition we will to increase our understanding of mechanotransduction in Merkel cells, an epidermal mechano-sensory cell. The BCMProbe will be a new instrument at Baylor College of Medicine and its design is based on over 10 years of experience gained in constructing a previous model. The new instrument will be enhanced by super-resolution microscopy, electro-optic deflectors and complete system integration. The design is modular so that a variety of user specific experiments such as calcium imaging, fluorescence recovery after photobleaching or fluorescence resonance energy transfer may be easily accommodated. Our users have extensive expertise in optical approaches to membrane biophysics and mechanotransduction in sensory systems. The work will have implications beyond the hearing and somatosensory systems as it will reveal fundamental membrane mechanisms. These mechanisms have implications for understanding membrane transport and even learning and memory. While the obvious beneficiaries of the instrument will be the hard of hearing and individuals with touch disorders, solutions for other clinical problems will undoubtedly arise because every cell and the organelles within are enclosed by a membrane.

描述（由申请人提供）：要求生物物理细胞膜探针（BCMPROBE）扩大有关活细胞膜的生物物理研究。 BCMPROBE由与电生理贴片钳模块和驻波显微镜集成的光学镊子组成。 BCMPROBE是一种新颖的独特仪器，可用于调节膜力学以及跨越电场的大小。同时，它可以测量膜和膜电流产生的力。主要用户将利用该仪器来进一步检查纳米级膜管中的膜机电和肌动蛋白捆绑，测量与细胞变形相关的电荷运动，并检查自我关联以及整体膜蛋白的迁移率。我们将能够扩展我们以前的工作，该工作表明了能够将电能直接转化为声学频率的机械能的细胞膜。此外，我们将增加对表皮机械感官默克尔细胞机械转导的理解。 BCMPROBE将是贝勒医学院的一种新工具，其设计基于在建造以前的模型中获得的10多年的经验。新仪器将通过超分辨率显微镜，电磁偏转器和完整的系统集成来增强新仪器。该设计是模块化的，因此很容易容易容纳各种用户特定的实验，例如钙成像，光漂白后的荧光回收或荧光共振能量传递。我们的用户在感觉系统中的膜生物物理学和机械转移方面具有广泛的专业知识。这项工作将在听证会和体感系统之外具有影响，因为它将揭示基本的膜机制。这些机制对了解膜运输，甚至学习和记忆具有影响。尽管该仪器的明显受益者将是听力障碍和触摸障碍的人的难题，但其他临床问题的解决方案无疑会出现，因为每个细胞和内部细胞内的细胞都被膜包围。