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Biophysics Cell Membrane Probe

生物物理细胞膜探针

基本信息

批准号：
7794146
负责人：
WILLIAM E BROWNELL
金额：
$ 35.23万
依托单位：
BAYLOR COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-07-20 至 2011-07-19
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): A Biophysics Cell Membrane Probe (BCMProbe) is requested to expand biophysical studies on the membranes of living cells. The BCMProbe consists of optical tweezers integrated with an electrophysiology patch-clamp module and a standing wave microscope. The BCMProbe is a novel, unique instrument that can be used to modulate membrane mechanics as well as the magnitude of the electric field across it. At the same time it can measure the force produced by the membrane and the membrane current. Major users will exploit the instrument to further examine membrane electromechanics and actin bundling in nanoscale membrane tubes, measure charge movement associated with cell deformation and examine the self association as well as the mobility of integral membrane proteins. We will be able to expand our previous work that has demonstrated cell membranes capable of converting electrical energy directly into mechanical energy at acoustic frequencies. In addition we will to increase our understanding of mechanotransduction in Merkel cells, an epidermal mechano-sensory cell. The BCMProbe will be a new instrument at Baylor College of Medicine and its design is based on over 10 years of experience gained in constructing a previous model. The new instrument will be enhanced by super-resolution microscopy, electro-optic deflectors and complete system integration. The design is modular so that a variety of user specific experiments such as calcium imaging, fluorescence recovery after photobleaching or fluorescence resonance energy transfer may be easily accommodated. Our users have extensive expertise in optical approaches to membrane biophysics and mechanotransduction in sensory systems. The work will have implications beyond the hearing and somatosensory systems as it will reveal fundamental membrane mechanisms. These mechanisms have implications for understanding membrane transport and even learning and memory. While the obvious beneficiaries of the instrument will be the hard of hearing and individuals with touch disorders, solutions for other clinical problems will undoubtedly arise because every cell and the organelles within are enclosed by a membrane.

描述（由申请人提供）：生物物理学细胞膜探针（BCMProbe）用于扩大活细胞膜的生物物理学研究。BCM探针由集成了电生理膜片钳模块和驻波显微镜的光镊组成。BCMProbe是一种新颖独特的仪器，可用于调节膜力学以及跨膜电场的大小。同时，它可以测量膜产生的力和膜电流。主要用户将利用该仪器进一步研究纳米膜管中的膜电动力学和肌动蛋白捆绑，测量与细胞变形相关的电荷运动，并研究自缔合以及完整膜蛋白的流动性。我们将能够扩展我们以前的工作，已经证明细胞膜能够将电能直接转换为声频机械能。此外，我们将增加我们对默克尔细胞，一种表皮机械感觉细胞中的机械传导的理解。BCMProbe将是贝勒医学院的一种新仪器，其设计基于10多年来构建先前模型的经验。新仪器将通过超分辨率显微镜、电光偏转器和完整的系统集成来增强。该设计是模块化的，使得可以容易地适应各种用户特定的实验，例如钙成像、光漂白后的荧光恢复或荧光共振能量转移。我们的用户在膜生物物理学的光学方法和感觉系统中的机械转导方面具有广泛的专业知识。这项工作的意义将超越听觉和体感系统，因为它将揭示基本的膜机制。这些机制对理解膜转运甚至学习和记忆都有意义。虽然该仪器的明显受益者将是听力障碍和触觉障碍的人，但毫无疑问，其他临床问题的解决方案也会出现，因为每个细胞和细胞器都被膜包围。