Purchase of an Isothermal Titration Calorimeter

购买等温滴定量热计

• 批准号: 7792160
• 负责人: Robert T Batey
• 金额: $ 12.88万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7792160
• 关键词: Affinity; Arts; Binding; Biochemistry; Biophysics; Calorimetry; Chromosomes; Colorado; Communities; DNA-Protein Interaction; Entropy; FDA approved; Funding; Generations; Goals; Grant; Health; Heating; Human; Kinetics; Label; Macular degeneration; Measures; Minor; Nature; Nucleic Acids; Pharmaceutical Preparations; Phosphotransferases; Reaction; Regulatory Element; Request for Proposals; Research; Running; Sampling; Signal Transduction Pathway; Techniques; Time; Titrations; Universities; aptamer; base; enthalpy; fighting; instrument; instrumentation; programs; research study; stoichiometry

DESCRIPTION (provided by applicant): This shared instrumentation grant program proposal requests funds for the purchase of a state-of-the-art isothermal titration microcalorimeter (ITC). Calorimetry provides a wealth of information about macromolecular interactions and enzymatic kinetics by measuring the heats of reaction. This technique has distinct advantages over others, including that it can obtain information about the binding affinity, enthalpy and entropy of reaction and stoichiometry of the reaction in a single experiment and that it does not require the labeling of either the titrant or titrate molecules. Recent advances in instrumentation also allow for 10-fold lower sample amounts to be used per experiment and significantly decreased run time as compared to previous generation instruments. These considerations make this technique accessible to a broader spectrum of users and facilitates overall higher throughput, which are important features for shared instrumentation. Acquisition of the proposed MicroCal iTC200 would facilitate research programs directly related to human health issues in a number of important ways. Major users of this instrument propose to use this instrument to investigate the regulatory mechanisms of kinases involved in a variety of key cellular signal transduction pathways in humans (Prof. Natalie Ahn), RNA-based regulatory elements that directly sense cellular metabolites (Prof. Robert Batey), a nucleic acid aptamer that is an FDA- approved drug to fight macular degeneration (Prof. Arthur Pardi), and the nature of protein-DNA interactions at the end of linear chromosomes (Prof. Deborah Wuttke). These research goals along with those of the minor users emphasizes the strength of biophysics and biochemistry at the University of Colorado, Boulder and the proposed instrument would fulfill a core need of a large community of users.

描述（由申请人提供）：此共享仪器赠款计划提案要求购买最先进的等温滴定微钙化计（ITC）的资金。通过测量反应热，量热法提供了有关大分子相互作用和酶促动力学的大量信息。该技术比其他技术具有不同的优势，包括它可以在单个实验中获得有关反应的结合亲和力，焓和反应的熵和化学计量的信息，并且它不需要标记滴定剂或滴定分子。仪器的最新进展还允许通过实验使用10倍的样品量，并且与上一代工具相比，运行时间显着减少。这些考虑使得该技术可用于更广泛的用户，并促进了总体上更高的吞吐量，这是共享仪器的重要功能。拟议的Microcal ITC200的获取将以多种重要方式促进与人类健康问题直接相关的研究计划。该仪器的主要用户建议使用该工具研究人类（Natalie Ahn教授），基于RNA的调节元素，这些激酶的调节机制直接感知细胞代谢物（Robert Batey教授），apti and arneci and arnate pros and Figrual and prifors and priarguart and produla线性染色体结束时蛋白-DNA相互作用的性质（Deborah Wuttke教授）。这些研究目标以及未成年人的研究目标强调了科罗拉多大学博尔德大学的生物物理学和生物化学的力量，而拟议的工具将满足大量用户社区的核心需求。