Purchase of an Isothermal Titration Calorimeter

购买等温滴定量热计

• 批准号: 7792160
• 负责人: Robert T Batey
• 金额: $ 12.88万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7792160
• 关键词: Affinity; Arts; Binding; Biochemistry; Biophysics; Calorimetry; Chromosomes; Colorado; Communities; DNA-Protein Interaction; Entropy; FDA approved; Funding; Generations; Goals; Grant; Health; Heating; Human; Kinetics; Label; Macular degeneration; Measures; Minor; Nature; Nucleic Acids; Pharmaceutical Preparations; Phosphotransferases; Reaction; Regulatory Element; Request for Proposals; Research; Running; Sampling; Signal Transduction Pathway; Techniques; Time; Titrations; Universities; aptamer; base; enthalpy; fighting; instrument; instrumentation; programs; research study; stoichiometry

DESCRIPTION (provided by applicant): This shared instrumentation grant program proposal requests funds for the purchase of a state-of-the-art isothermal titration microcalorimeter (ITC). Calorimetry provides a wealth of information about macromolecular interactions and enzymatic kinetics by measuring the heats of reaction. This technique has distinct advantages over others, including that it can obtain information about the binding affinity, enthalpy and entropy of reaction and stoichiometry of the reaction in a single experiment and that it does not require the labeling of either the titrant or titrate molecules. Recent advances in instrumentation also allow for 10-fold lower sample amounts to be used per experiment and significantly decreased run time as compared to previous generation instruments. These considerations make this technique accessible to a broader spectrum of users and facilitates overall higher throughput, which are important features for shared instrumentation. Acquisition of the proposed MicroCal iTC200 would facilitate research programs directly related to human health issues in a number of important ways. Major users of this instrument propose to use this instrument to investigate the regulatory mechanisms of kinases involved in a variety of key cellular signal transduction pathways in humans (Prof. Natalie Ahn), RNA-based regulatory elements that directly sense cellular metabolites (Prof. Robert Batey), a nucleic acid aptamer that is an FDA- approved drug to fight macular degeneration (Prof. Arthur Pardi), and the nature of protein-DNA interactions at the end of linear chromosomes (Prof. Deborah Wuttke). These research goals along with those of the minor users emphasizes the strength of biophysics and biochemistry at the University of Colorado, Boulder and the proposed instrument would fulfill a core need of a large community of users.

描述(由申请人提供)：这项共享仪器拨款计划申请资金用于购买最先进的等温滴定微量热计(ITC)。量热法通过测量反应热，提供了关于大分子相互作用和酶动力学的丰富信息。这项技术与其他技术相比具有明显的优势，包括它可以在一次实验中获得关于反应的结合亲和力、反应的焓和熵以及反应的化学计量比的信息，并且它不需要标记滴定剂或滴定物分子。与上一代仪器相比，仪器的最新进展还允许每个实验使用的样本量减少10倍，并显著减少运行时间。这些考虑因素使更广泛的用户可以访问该技术，并促进总体更高的吞吐量，这是共享仪器的重要功能。收购拟议中的MicroCal iTC200将在许多重要方面促进与人类健康问题直接相关的研究计划。该仪器的主要使用者建议使用该仪器来研究参与人类各种关键细胞信号转导通路的激酶的调控机制(Natalie Ahn教授)，直接感受细胞代谢物的基于RNA的调控元件(Robert Batey教授)，FDA批准的抗黄斑变性药物核酸适体(Arthur Pardi教授)，以及线性染色体末端蛋白质与DNA相互作用的性质(Deborah Wuttke教授)。这些研究目标以及小用户的目标强调了科罗拉多大学博尔德分校的生物物理和生物化学的优势，所建议的仪器将满足大量用户的核心需求。