Purchase of an Isothermal Titration Calorimeter

购买等温滴定量热计

• 批准号: 7792160
• 负责人: Robert T Batey
• 金额: $ 12.88万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7792160
• 关键词: Affinity; Arts; Binding; Biochemistry; Biophysics; Calorimetry; Chromosomes; Colorado; Communities; DNA-Protein Interaction; Entropy; FDA approved; Funding; Generations; Goals; Grant; Health; Heating; Human; Kinetics; Label; Macular degeneration; Measures; Minor; Nature; Nucleic Acids; Pharmaceutical Preparations; Phosphotransferases; Reaction; Regulatory Element; Request for Proposals; Research; Running; Sampling; Signal Transduction Pathway; Techniques; Time; Titrations; Universities; aptamer; base; enthalpy; fighting; instrument; instrumentation; programs; research study; stoichiometry

DESCRIPTION (provided by applicant): This shared instrumentation grant program proposal requests funds for the purchase of a state-of-the-art isothermal titration microcalorimeter (ITC). Calorimetry provides a wealth of information about macromolecular interactions and enzymatic kinetics by measuring the heats of reaction. This technique has distinct advantages over others, including that it can obtain information about the binding affinity, enthalpy and entropy of reaction and stoichiometry of the reaction in a single experiment and that it does not require the labeling of either the titrant or titrate molecules. Recent advances in instrumentation also allow for 10-fold lower sample amounts to be used per experiment and significantly decreased run time as compared to previous generation instruments. These considerations make this technique accessible to a broader spectrum of users and facilitates overall higher throughput, which are important features for shared instrumentation. Acquisition of the proposed MicroCal iTC200 would facilitate research programs directly related to human health issues in a number of important ways. Major users of this instrument propose to use this instrument to investigate the regulatory mechanisms of kinases involved in a variety of key cellular signal transduction pathways in humans (Prof. Natalie Ahn), RNA-based regulatory elements that directly sense cellular metabolites (Prof. Robert Batey), a nucleic acid aptamer that is an FDA- approved drug to fight macular degeneration (Prof. Arthur Pardi), and the nature of protein-DNA interactions at the end of linear chromosomes (Prof. Deborah Wuttke). These research goals along with those of the minor users emphasizes the strength of biophysics and biochemistry at the University of Colorado, Boulder and the proposed instrument would fulfill a core need of a large community of users.

描述（由申请人提供）：本共享仪器资助计划提案要求购买最先进的等温滴定微热计（ITC）。量热法通过测量反应热提供了大量关于大分子相互作用和酶动力学的信息。与其他技术相比，这种技术具有明显的优势，包括它可以在一次实验中获得有关反应的结合亲和力、焓和熵以及反应的化学计量学的信息，而且它不需要标记滴定剂或滴定剂分子。与上一代仪器相比，仪器的最新进展还允许每次实验使用的样本量降低10倍，运行时间显着缩短。这些考虑因素使更广泛的用户可以使用该技术，并促进总体上更高的吞吐量，这是共享仪器的重要特性。收购拟议的MicroCal iTC200将在许多重要方面促进与人类健康问题直接相关的研究项目。该仪器的主要用户建议使用该仪器来研究人类多种关键细胞信号转导途径中涉及的激酶的调节机制（Natalie Ahn教授），直接感知细胞代谢物的基于rna的调节元件（Robert Batey教授），FDA批准的抗黄斑变性药物核酸适配体（Arthur Pardi教授），以及线性染色体末端蛋白质- dna相互作用的性质（Deborah Wuttke教授）。这些研究目标以及次要用户的研究目标强调了科罗拉多大学博尔德分校生物物理学和生物化学的实力，并且提议的仪器将满足大量用户的核心需求。