NCRR Shared Instrumentation Grant : Bioluminescent Scanner

描述（由申请人提供）：体内、真实的时间和细胞水平的基因表达的分子成像是医学研究的一个快速增长的领域，其将提供对遗传水平的疾病的起源和进展的洞察。用于非侵入性纵向监测活体动物的疾病进展、细胞运输和基因表达模式的仪器现已问世。这种模式将提供以前不可能与经典的组织病理学技术的见解。Xenogen公司的IVIS体内生物发光成像系统被公认为处于这一新兴领域的最前沿。在过去的四年里，我们一直在运行这样一个系统，作为一个名为体内分子成像实验室（IMIL）的共享研究设施。目前的系统是Xenogen IVIS 200，是在2005年底购买的，使用了12个系主任基金的捐款和医学院院长的配套捐款。在此期间，我们有一个非常顺利和成功的运作，并支持了许多部门导致赠款和论文。虽然在购买时是最先进的，但这项技术近年来已经取得了进展。在这个应用程序中，我们正在寻求从同一家公司申请资金升级到下一代系统。新系统被称为Spectrum。这一新系统是这一模式中的最新产品，具有更高的分辨率以及更多用于成像额外光波长的滤波器。Spectrum可以作为新设备购买，但我们目前的IVIS 200有一个升级路径，使我们能够以一小部分成本获得Spectrum。 公共卫生相关性：疾病的动物模型在医学研究中至关重要。生物发光成像允许研究人员使用小动物疾病模型来监测体内疾病进展和细胞功能。

DESCRIPTION (provided by applicant): Molecular imaging of gene expression in vivo, in real time and at the cellular level is a rapidly growing area of medical research that will provide insight into the origin and progression of disease at the genetic level. Instrumentation for non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in life animals are now available. This modality will provide insights not previously possible with classical histopathology techniques. The Xenogen Corporation's IVIS in vivo bioluminescence imaging system is recognized as being at the forefront of this emerging field. We have been operating such a system for the past four years as a shared research facility under the name In-Vivo Molecular Imaging Lab (IMIL). The current system which is a Xenogen IVIS 200 was purchased in late 2005 using contributions from 12 department chairs funds and matching contributions from the dean of the medical school. During this time we have had a very smooth and successful operation and have supported many departments resulting in grants and papers. Although state of the art at the time of purchase, this technology has advanced in recent times. In this application we are seeking to apply for funds for an upgrade to the next generation system from the same company. The new system is called Spectrum. This new system is the latest in this modality with improved resolution as well as more filters for imaging of additional optical wavelengths. The Spectrum can be purchased as a new unit, but our current IVIS 200 has an upgrade path that allows us to obtain the Spectrum at a fraction of the cost. Public Health Relevance: Animal models of disease are essential in medical research. Bioluminescent imaging allows researchers with small animal models of disease to monitor in-vivo disease progression and cellular function.