High-End Instrumentation Grant Program

高端仪器资助计划

• 批准号: 7497342
• 负责人: CHEUK Y TANG
• 金额: $ 199.9万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7497342
• 关键词: Alzheimer' s Disease; Animal Disease Models; Animal Model; Animals; Arterial Fatty Streak; Biological Markers; Biological Process; Brain; Caliber; Center for Translational Science Activities; Complement; Contrast Media; Development; Devices; Diagnosis; Diffusion Magnetic Resonance Imaging; Disease; Foundations; Funding; Future; Genetically Modified Animals; Grant; Human; Image; Institutes; Institution; Laboratories; Magnetic Resonance Imaging; Methods; Modality; Morphology; Mus; Oryctolagus cuniculus; Pharmacologic Substance; Preclinical Drug Development; Primates; Process; RF coil; Rattus; Research Institute; Research Personnel; Research Project Grants; Resolution; Rodent; Running; Scanning; Schizophrenia; Specificity; Specimen; Structure; System; TNFRSF5 gene; Testing; Translating; Translations; base; clinical practice; disease mechanisms study; imaging modality; in vivo; instrument; instrumentation; mouse model; programs; public health relevance; quantum; receptor; soft tissue; treatment strategy; white matter

DESCRIPTION (provided by applicant): In vivo imaging of small animals form the foundation for any translational research center. In recent years, microimaging devices have taken a quantum leap in terms of both their number in use at pharmaceutical or academic institutes and improvement in their sensitivity, resolution and other technical breakthroughs. MicroMRI may be the most versatile of these modalities with the ability to obtain superior soft tissue contrast, function and resolution. We propose to acquire a Bruker 7 T ClinScan MRI scanner, capable of obtaining in vivo high-resolution images of mice, other laboratory rodents, rabbits, and small primates. The purpose of this instrument is to allow researchers at our institute to study morphology of wild-type or genetically modified animals, study preclinical drug development or other treatment strategies, detect biomarkers of disease and to develop new MRI based contrast agents to various study disease mechanisms. Our research institute currently has one Bruker 9.4 T installed. This is a small bore magnet which only allow imaging of mice. In addition, this scanner is already used beyond its capacity by a large number of projects. Our institute has received several new grants that require microMRI which makes it critical that we enhance our capacity. The 7 T ClinScan will benefit several current PIs who are conducting microMRI studies as well new PIs with new animal models. The 7 T ClinScan complements our current 9.4 T in several ways: the 7 T has a much larger bore size which allows imaging of rats and small primates. It would also allow whole human brain specimen to be imaged. Our institute has a large brain bank and the 7 T would allow non-destructive documenting of these specimens. Several federally funded grants will benefit from this new scanner in terms of its expanded capabilities. Our current 9.4 T with a 30 mm inner diameter RF coil is only capable of imaging mice and is currently serving several R01, P01, and P50 projects as well as some pilot scans. It is currently already running 24/7. The 7 T ClinScan can accommodate RF coils with inner diameter of 15.4 cm which allows imaging of small primates as well as whole brain specimens. We also foresee that the 7 T field strength will become a standard in clinical practice in the near future, so it is important that mechanistic issues about disease processes be studied at this level to allow translation. Figure 1 shows currently installed 7 T systems. Research projects at our institution include white matter disruption in schizophrenia using genetically modified mouse models and diffusion tensor imaging, high resolution morphometric studies of mouse models of Alzheimer's disease, development of MRI contrast agents both for in vivo imaging of certain brain receptors and other biomarkers as well as atherosclerotic plaque imaging. PUBLIC HEALTH RELEVANCE: MicroMRI is the superior imaging modality to visualize soft tissue structure as well as biological processes. A microMRI scanner will allow researchers to test efficiently animal models of disease and treatment strategies at much higher resolution and specificity than is possible with human scanners. Results can be translated conveniently to human MRI scanners in terms of diagnosis as well as to humans in terms of treatment methods.

描述（由申请人提供）：小动物的体内成像是任何转化研究中心的基础。近年来，显微成像设备在制药或学术机构的使用数量以及灵敏度，分辨率和其他技术突破方面都取得了巨大的飞跃。MicroMRI可能是这些模式中最通用的，能够获得上级软组织对比度，功能和分辨率。我们建议购买Bruker 7 T ClinScan MRI扫描仪，该扫描仪能够获得小鼠、其他实验室啮齿动物、兔子和小型灵长类动物的体内高分辨率图像。该仪器的目的是使我们研究所的研究人员能够研究野生型或转基因动物的形态学，研究临床前药物开发或其他治疗策略，检测疾病的生物标志物，并开发新的基于MRI的造影剂以研究各种疾病机制。我们的研究所目前安装了一台Bruker 9.4 T。这是一个小口径磁铁，只允许小鼠成像。此外，这台扫描仪已经被大量项目超出其能力范围。我们的研究所已经收到了几个新的赠款，需要microMRI，这使得我们提高我们的能力至关重要。7 T ClinScan将使正在进行microMRI研究的几位当前PI以及使用新动物模型的新PI受益。7 T ClinScan在几个方面补充了我们目前的9.4 T：7 T具有更大的孔径，可以对大鼠和小型灵长类动物进行成像。它还可以对整个人脑样本进行成像。我们的研究所有一个大型的大脑银行和7 T将允许非破坏性记录这些标本。几个联邦资助的赠款将受益于这一新的扫描仪在其扩大的能力。我们目前的9.4 T，内径为30 mm的RF线圈仅能够对小鼠进行成像，目前正在为几个R 01，P01和P50项目以及一些试点扫描提供服务。目前，它已经运行24/7。7 T ClinScan可容纳内径为15.4 cm的RF线圈，可对小型灵长类动物以及全脑标本进行成像。我们还预见到，在不久的将来，7 T场强将成为临床实践的标准，因此，重要的是，在这个水平上研究疾病过程的机制问题，以允许翻译。图1显示了目前安装的7 T系统。在我们机构的研究项目包括精神分裂症的白色物质破坏使用转基因小鼠模型和扩散张量成像，高分辨率的阿尔茨海默氏病的小鼠模型的形态学研究，MRI造影剂的发展都在某些脑受体和其他生物标志物的体内成像以及动脉粥样硬化斑块成像。 公共卫生相关性：MicroMRI是可视化软组织结构以及生物过程的上级成像模式。microMRI扫描仪将使研究人员能够以比人类扫描仪更高的分辨率和特异性有效地测试疾病和治疗策略的动物模型。结果可以方便地转换为人类MRI扫描仪在诊断方面以及人类在治疗方法方面。