Acquisition of a Multiphoton Microscope for Cellular Programming

获取用于细胞编程的多光子显微镜

• 批准号: 7793841
• 负责人: DAVID F MEANEY
• 金额: $ 50万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793841
• 关键词: 12 year old; Applied Research; Area; Arts; Biomedical Engineering; Cartilage; Cell Culture Techniques; Cells; Complex; Dental; Disease; Drug Formulations; Engineering; Etiology; Hippocampus (Brain); Injury; Life; Messenger RNA; Microscope; Natural regeneration; Optics; Organism; Pennsylvania; Pharmacology; Phenotype; Population; Regenerative Medicine; Research; Schools; Science; System; Techniques; Technology; Therapeutic; Tissues; Universities; Work; base; in vivo; instrumentation; medical schools; meetings; nervous system disorder; neural circuit; neurobehavior; new technology; novel; programs

DESCRIPTION (provided by applicant): This shared instrumentation proposal for a customized multiphoton microscope is part of a larger institutional effort at Penn in cellular programming. Strategically, this effort lies at the intersection between five of Penn's schools - Medicine, Dental, Veterinary, Arts and Sciences, and Engineering and Applied Science. We request a multiphoton microscope equipped to perform three separate, but related efforts, in cellular programming. The efforts include: At the single cell level, where we use a novel technology developed by one of the project PIs (Jim Eberwine, Pharmacology, School of Medicine) to controllably deliver a define mRNA population to living cells to redirect their cellular phenotype, At the multicellular scale, where we use novel photopolymer formulations to assemble complex, three-dimensional cell culture substrates (Chris Chen, Bioengineering, School of Engineering and Applied Science) with tunable microenvironments for building vascularized tissue and cartilage, and At the tissue scale, where we use widely available optical activation techniques to study the in vivo programming of neural circuits in the cortex and hippocampus to understand changes that occur during disease or injury (David Meaney, Bioengineering, School of Engineering and Applied Science). This new microscope system will replace an existing 12 year old BioRad multiphoton microscope in the engineering complex at the University of Pennsylvania. The current BioRad system does meet the high technical demands of the above applications. Moreover, there is no widely available existing system on the Penn campus to perform this work. Therefore, there is substantial need for this microscope system. The combination of these three 'base technologies' on one microscope platform can significantly advance research topics in broadly diverse areas such as cellular differentiation, regenerative medicine, and the etiology of neurological disease and neurobehavior. The potential of integrating two or three of the base technologies into a single topic area provides nearly limitless possibilities for cutting edge advances in how living systems form and regenerate tissue, as well as developing a platform for assembling novel tissue replacement or RNA-based therapeutic approaches.

描述（由申请人提供）：这种定制多光子显微镜的共享仪器建议是宾夕法尼亚大学细胞编程中更大机构努力的一部分。从战略上讲，这一努力是在宾夕法尼亚大学的五个学校之间的交叉点-医学，牙科，兽医，艺术和科学，工程和应用科学。我们要求一个多光子显微镜，配备执行三个独立的，但相关的努力，在细胞编程。这些努力包括：在单细胞水平上，我们使用了一项由项目PI开发的新技术（Jim Eberwine，药理学，医学院）可控地将确定的mRNA群体递送至活细胞以重定向其细胞表型，在多细胞规模下，我们使用新型光聚合物制剂组装复杂的三维细胞培养基质，（Chris Chen，生物工程，工程与应用科学学院）用可调微环境构建血管化组织和软骨，并在组织规模上，在那里，我们使用广泛使用的光学激活技术来研究大脑皮层和海马体中神经回路的体内编程，疾病或损伤期间发生的变化（大卫米尼，生物工程，工程和应用科学学院）。这种新的显微镜系统将取代宾夕法尼亚大学工程综合体中现有的已有12年历史的BioRad多光子显微镜。目前的BioRad系统确实满足上述应用的高技术要求。此外，在宾夕法尼亚大学校园内没有广泛使用的现有系统来执行这项工作。因此，对这种显微镜系统有很大的需求。在一个显微镜平台上结合这三种“基础技术”可以显着推进广泛领域的研究课题，如细胞分化，再生医学，神经疾病和神经行为的病因学。将两种或三种基础技术整合到一个主题领域的潜力为生命系统如何形成和再生组织的前沿进展提供了几乎无限的可能性，并为组装新型组织替代或基于RNA的治疗方法提供了平台。