Pulsed Q-band EPR Spectrometer

脉冲 Q 波段 EPR 光谱仪

• 批准号: 7794046
• 负责人: ALBERT H BETH
• 金额: $ 48.25万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-18 至 2012-05-01
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794046
• 关键词: Area; Binding Proteins; Complement; Data; Deer; Equipment; Funding; Funding Applicant; Laboratories; Measurement; Measures; Membrane; Membrane Proteins; Methodology; Noise; Physiologic pulse; Positioning Attribute; Proteins; Relative (related person); Request for Applications; Research; Research Personnel; Sampling; Signal Transduction; Site; Spin Labels; Structure; System; Time; United States National Institutes of Health; Universities; experience; instrumentation; operation; orientation selectivity; reconstitution

DESCRIPTION (provided by applicant): Funds are requested to complete the purchase of a Bruker EleXsys 580 Q-band FT-EPR system at Vanderbilt University. This equipment will be utilized by five different established research groups and a number of their collaborators to make DEER measurements on a wide range of nitroxide spin-labeled proteins that are already being studied using site-directed spin-labeling. Recent advances in instrumentation and methodology have positioned time-domain EPR as an essential complement to cw-EPR for characterizing the structures and dynamics of spin-labeled proteins. In the ten NIH funded projects that constitute the core user group of this application, the requested equipment will be utilized to: 1) increase our capacity for measuring "long" (> 20 - 25 E) inter-probe distances in proteins by DEER; 2) increase signal to noise for small samples including reconstituted membrane proteins; 3) permit multifrequency analysis of DEER data; 4) provide orientation selectivity for assessment of relative orientations between spin labels; and 5) provide the capability to make reliable cw-Q-band measurements. In each of the five applicant laboratories, the requested instrumentation will have an immediate and substantial impact by providing unique new measurement capabilities for full characterization of the structures and dynamics of wide range of soluble and membrane bound proteins that are of clear biomedical importance. The requested instrumentation will also be made available to other investigators at Vanderbilt and in the surrounding area as they develop new projects that involve spin-labeling. Equitable utilization of the equipment will be assured by an internal oversight committee and day-to-day operation will be handled by an experienced EPR spectroscopist.

描述（由申请人提供）：申请资金用于在范德比尔特大学完成购买Bruker EleXsys 580 Q波段FT-EPR系统。该设备将由五个不同的研究小组和他们的一些合作者使用，对已经使用定点自旋标记研究的各种氮氧自旋标记蛋白质进行DEER测量。在仪器和方法学的最新进展定位的时域EPR作为一个必不可少的补充，顺式EPR的结构和动力学的自旋标记蛋白质的表征。在构成该应用程序核心用户组的十个NIH资助项目中，所要求的设备将用于：1）增加我们测量“长”的能力。（> 20 - 25 E）通过DEER在蛋白质中的探针间距离; 2）增加包括重构膜蛋白的小样品的信噪比; 3）允许DEER数据的多频率分析; 4）提供取向选择性以评估自旋标记之间的相对取向;以及5）提供进行可靠的cw-Q带测量的能力。在五个申请实验室中的每一个实验室中，所要求的仪器将通过提供独特的新测量能力来全面表征具有明显生物医学重要性的各种可溶性和膜结合蛋白质的结构和动力学，从而产生直接和实质性的影响。所要求的仪器也将提供给其他研究人员在范德比尔特和周围地区，因为他们开发新的项目，涉及自旋标记。一个内部监督委员会将确保设备的公平使用，日常操作将由一名经验丰富的EPR光谱师处理。