Time Domain EPR Spectrometer

时域 EPR 光谱仪

• 批准号: 6877230
• 负责人: ALBERT H BETH
• 金额: $ 50万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-15 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877230
• 关键词: biomedical equipment purchase; electron spin resonance spectroscopy

DESCRIPTION (provided by applicant): Funds are requested to complete the purchase of a Bruker EleXsys 580 FT-EPR system at Vanderbilt University. This equipment, which is not currently available on campus or at any nearby institution, will be utilized by six different established research groups to make time-domain EPR measurements on a wide range of nitroxide spin-labeled proteins that are already being studied using conventional cw-EPR approaches and site-directed spin-labeling. Recent advances in instrumentation and methodology have positioned timedomain EPR as an important and in many cases an essential complement to cw-EPR for characterizing the structures and dynamics of spin-labeled proteins. In the eight NIH funded projects that constitute the core user group of this application, the requested equipment will be utilized to: 1) measure "long" (> 20 - 25 angstroms) inter-probe distances in proteins by DEER and/or double quantum coherence; 2) measure T1e's of spin-labeled side chains in the presence and absence of paramagnetic relaxation agents as a direct index of side-chain accessibility; and 3) measure T1e's for spin-labeled proteins that are being studied by non-linear methods including saturation transfer EPR. In each of the eight ongoing projects, the requested instrumentation will have an immediate and substantial impact by providing unique new measurement capabilities for full characterization of the structures and dynamics of wide range of soluble and membrane bound proteins that are of clear biomedical importance, The requested instrumentation will also be made available to other investigators at Vanderbilt and in the surrounding area as they develop new projects that involve spinlabeling. Equitable utilization of the equipment will be assured by an internal oversight committee and day to- day operation will be handled by an experienced EPR spectroscopist.

描述（由申请人提供）： 申请资金用于完成范德比尔特大学购买Bruker EleXsys 580 FT-EPR系统。该设备目前在校园或附近的任何机构都不可用，将由六个不同的研究小组使用，对已经使用传统的cw-EPR方法和定点自旋标记研究的各种氮氧自旋标记蛋白质进行时域EPR测量。仪器和方法的最新进展已经定位时域EPR作为一个重要的，在许多情况下，一个必不可少的补充cw-EPR表征自旋标记蛋白质的结构和动力学。在构成本申请核心用户群的八个NIH资助项目中，所要求的设备将用于：1）测量“长”（> 20 - 25埃）通过DEER和/或双量子相干测量蛋白质中的探针间距离; 2）在存在和不存在顺磁弛豫剂的情况下测量自旋标记的侧链的T1 e，作为侧链可及性的直接指标;和3）测量通过非线性方法包括饱和转移EPR研究的自旋标记蛋白质的T1 e。在八个正在进行的项目中，所要求的仪器将产生直接和实质性的影响，提供独特的新的测量能力，全面表征具有明显生物医学重要性的各种可溶性和膜结合蛋白质的结构和动力学，在范德比尔特和周边地区的其他研究人员开发新项目时，也将提供所需的仪器涉及到自旋标记。设备的公平使用将由一个内部监督委员会保证，日常操作将由一名经验丰富的EPR光谱师处理。