Live Cell Imaging Confocal Microscope System

活细胞成像共焦显微镜系统

• 批准号: 7795474
• 负责人: Jeffrey Shawn Goodwin
• 金额: $ 41.87万
• 依托单位: MEHARRY MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-03 至 2011-06-02
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7795474
• 关键词: AIDS/HIV problem; Academic Medical Centers; Address; Area; Arts; Biological; Cardiovascular Diseases; Cells; Core Facility; Data; Development; Equipment; Faculty; Fluorescence Resonance Energy Transfer; Fluorescent Probes; Funding; Goals; Housing; IACUC; Image; Individual; Institution; Label; Leadership; Learning; Life; Malignant Neoplasms; Microscope; Minor; Minority; Mission; Monitor; Morphology; Peer Review; Process; Productivity; Research; Research Ethics Committees; Research Personnel; Resolution; Scanning; Science; Specimen; Speed; System; Technical Expertise; Technology; Time; Training; Women' s Health; brain behavior; cellular imaging; college; experience; health disparity; instrument; instrumentation; medical schools; meetings; member; next generation; novel; programs; trafficking

DESCRIPTION (provided by applicant): Meharry Medical College investigators and their trainees have an immediate need for a confocal microscope capable of high speed as well as long-term imaging to capture dynamic processes occurring within living cells. These studies include examining interactions between fluorescently tagged individual molecules as well as examining native and regulated trafficking of one or several simultaneously labeled molecules from one intracellular compartment to another. The studies proposed use state of the art technologies introduced over the last several years (FRET, FLIP, FRAP, etc.) which are still evolving, as well as novel fluorescent probes that can be photoactivated or photoconverted in discrete regions within cells, and monitored over time. No equipment with the required features for addressing the hypotheses of our faculty members' peer-reviewed and funded studies exists on campus. To meet the needs of our investigators and their trainees, we are proposing purchase of the Nikon A1R confocal microscope for installation in our Morphology Core Facility, which is under the leadership of the PI, Dr. J. Shawn Goodwin, who is experienced in all of the proposed technologies and participates in their refinement nationally. Meharry's investigators focus on health disparities research in the areas of Women's Health (including cardiovascular diseases), HIV/AIDS, Cancer, and Brain and Behavior. Though our investigators can obtain pilot data on similar instrumentation at Vanderbilt University Medical Center, also in Nashville, Vanderbilt too lacks an instrument capable of both high resolution point scanning as well as high-speed, resonant scanning with an integrated perfect focus system. All of which are provided by the Nikon A1R confocal microscope. Furthermore, we have learned that routinely engaging in live cell imaging studies in an off campus setting is not conducive to the productivity of our faculty and their research programs, particularly in studies using freshly isolated or primary cultures derived from precious biological specimens under the purview of IACUC or IRB oversight. Equally importantly, the availability of this critical technology on campus is crucial for the mission of our institution to train the next generation of outstanding minority investigators in biomedical sciences. The long-range goal of the Morphology Core in which the Nikon A1R will be housed is to provide technical expertise and state-of-the-art instrumentation to enhance the productivity of Meharry's faculty investigators; in the pursuit of the mission of the Core and of the College, obtaining the Nikon A1R will benefit in a transformative fashion the peer-reviewed and funded research of the Major and Minor Users and the scientific development of their trainees.

描述（由申请人提供）：梅哈里医学院的研究人员和他们的学员迫切需要一种能够高速和长期成像的共聚焦显微镜，以捕获活细胞内发生的动态过程。这些研究包括检查荧光标记的单个分子之间的相互作用，以及检查一个或多个同时标记的分子从一个细胞内区室到另一个细胞内区室的天然和调节运输。这些研究建议使用过去几年引入的仍在不断发展的最先进技术（FRET、FLIP、FRAP 等），以及可以在细胞内离散区域进行光激活或光转换并随时间监测的新型荧光探针。校园内不存在具有解决我们教职员工同行评审和资助研究假设所需功能的设备。为了满足我们的研究人员及其学员的需求，我们建议购买 Nikon A1R 共焦显微镜，安装在我们的形态学核心设施中，该设施由 PI Shawn Goodwin 博士领导，他在所有拟议技术方面都有丰富的经验，并参与了全国范围内的改进。梅哈里的研究人员专注于女性健康（包括心血管疾病）、艾滋病毒/艾滋病、癌症以及大脑和行为领域的健康差异研究。尽管我们的研究人员可以在位于纳什维尔的范德比尔特大学医学中心获得类似仪器的试点数据，但范德比尔特大学也缺乏能够进行高分辨率点扫描以及具有集成完美聚焦系统的高速共振扫描的仪器。所有这些均由尼康 A1R 共焦显微镜提供。此外，我们了解到，在校外环境中定期进行活细胞成像研究不利于我们教师及其研究项目的生产力，特别是在使用来自 IACUC 或 IRB 监督范围内的珍贵生物标本的新鲜分离或原代培养物的研究中。同样重要的是，这项关键技术在校园内的可用性对于我们机构培养下一代生物医学领域杰出少数族裔研究人员的使命至关重要。尼康 A1R 所在的形态学核心的长期目标是提供技术专业知识和最先进的仪器，以提高 Meharry 教师研究人员的生产力；在追求核心和学院的使命的过程中，获得尼康 A1R 将以变革性的方式受益于主要和次要用户的同行评审和资助研究以及学员的科学发展。