Fluorescence Microscope Application

荧光显微镜应用

• 批准号: 7792628
• 负责人: Frank Joseph Giblin
• 金额: $ 10.78万
• 依托单位: OAKLAND UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-27 至 2011-05-26
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7792628
• 关键词: 12 year old; Aging; Biological Assay; Cartilage; Cataract; Cells; DNA Single Strand Break; Degenerative polyarthritis; Detection; Disease; Elderly; Epithelium; Fluorescence; Funding; Glaucoma; Goals; Grant; Health; Life; Population; Proteins; Research Personnel; Retina; Retinal Degeneration; Tissues; United States; United States National Institutes of Health; Universities; Work; age related; fluorescence microscope; instrument; lens

DESCRIPTION (provided by applicant): A group of five NIH-funded investigators at Oakland University (possessing five currently-funded NIH grants and one pending) request funds to purchase a Zeiss Motorized Axio Imager. Z1 Fluorescence Microscope. The requested instrument will replace a 12-year old, nearly obsolete Nikon fluorescence microscope that is currently being used by the researchers. The new instrument will greatly enhance the ability of the participating investigators to complete the aims of their NIH grants, and to follow any potentially exciting new leads. Types of fluorescence assays to be conducted with the new instrument include various types of immunolocalization assays in lens, retina and cartilage, detection of live and dead cells in lens epithelium, as well as determination of DNA single-strand breaks in that tissue, identification of glutathiolated proteins in lens, and assay of biomolecular fluorescence complementation in retina. The long-term goals of the proposed work are to determine the causes, and seek cures for certain common diseases of aging including glaucoma, cataract, age-related retinal degeneration and osteoarthritis. Each of these diseases represents a major health concern for the elderly population of the United States.

描述(由申请人提供)：奥克兰大学由NIH资助的五名研究人员(拥有五项NIH目前资助的赠款和一项待批资金)申请资金购买一台蔡司机动Axio成像仪。Z1荧光显微镜。所要求的仪器将取代研究人员目前使用的已有12年历史、几乎过时的尼康荧光显微镜。这一新工具将极大地提高参与调查人员完成他们的NIH拨款目标的能力，并追踪任何潜在的令人兴奋的新线索。新仪器将进行的荧光分析类型包括晶状体、视网膜和软骨中的各种免疫定位分析，晶状体上皮中活细胞和死亡细胞的检测，以及组织中DNA单链断裂的测定，晶状体中谷胱甘肽蛋白的鉴定，以及视网膜中生物分子荧光互补的测定。这项拟议工作的长期目标是确定病因，并寻求某些常见衰老疾病的治疗方法，包括青光眼、白内障、与年龄相关的视网膜退化和骨关节炎。这些疾病中的每一种都代表着美国老年人口的一个主要健康问题。

项目成果

PARP-1/PAR Activity in Cultured Human Lens Epithelial Cells Exposed to Two Levels of UVB Light.

暴露于两级 UVB 光的培养人晶状体上皮细胞中的 PARP-1/PAR 活性。

• DOI: 10.1111/php.12814
• 发表时间: 2018-01
• 期刊: Photochemistry and photobiology
• 影响因子: 3.3
• 作者: Cencer CS;Chintala SK;Townsend TJ;Feldmann DP;Awrow MA;Putris NA;Geno ME;Donovan MG;Giblin FJ
• 通讯作者: Giblin FJ