Vision Research Infrastructure Development Grant (R24)

愿景研究基础设施发展补助金 (R24)

• 批准号: 7188992
• 负责人: Frank Joseph Giblin
• 金额: $ 21.68万
• 依托单位: OAKLAND UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7188992
• 关键词: Area; Arts; Award; Biochemistry; Biological Sciences; Cancer Center Support Grant; Cataract; Cell Death; Cells; Communities; Confocal Microscopy; DNA; DNA Sequence; Development; Disease; Electron Microscopy; Environment; Equipment; Extracellular Matrix; Eye; Gene Expression; Glaucoma; Grant; Health; Histology; Hospital Departments; Hospitals; Image; Image Analysis; Light; Metabolism; Michigan; Microarray Analysis; Molecular; Molecular Biology; Monoclonal Antibody R24; Ophthalmologist; Ophthalmology; Participant; Photoreceptors; Research; Research Infrastructure; Research Institute; Research Personnel; Retinal; Retinal Degeneration; Role; Scanning Transmission Electron Microscopy Procedures; Sensory; Services; Structure; Techniques; Universities; Vision; Vision research; base; eye center; ganglion cell; lens; programs; tissue culture

DESCRIPTION (provided by applicant): The objective of this R24 application is to enhance the research capabilities of Oakland University's vision researchers, and promote collaborative research among the vision research community within the University and the affiliated William Beaumont Hospital, Department of Ophthalmology. Ten investigators, seven from the Eye Research Institute at O.U. and three from the O.U. Department of Biological Sciences, are designated as participants in the program. Six of the investigators hold independent NEI research awards, and one has a first-award application currently under review. Programs include lens and cataract, retinal biochemistry and metabolism in health and disease, the role of extracellular matrix in ganglion cell death in glaucoma, photoreceptor-related mechanisms of retinal degeneration and coordination of gene expression in retinal development. Support is requested for two Modules, Cell and Molecular Biology, and Ocular Structure and Imaging, in order to reorganize and expand existing Tissue Culture and Electron Microscopy/Histology Modules from a prior NEI Core Grant held by O.U. for the past 16 years. The Cell and Molecular Biology Module will provide assistance to O.U. vision researchers in the areas of tissue culture, basic molecular techniques, DNA sequencing and microarray technology, the latter in conjunction with the Sensory DNA Array Facility at the Kellogg Eye Center, University of Michigan. The Ocular Structure and Imaging Module will enhance the vision research environment of R24 participants by providing state-of-the-art capabilities for ultrastructure and imaging analysis using epifluorescence, routine light and confocal microscopy, as well as scanning and transmission electron microscopy. The availability of the requested facilities on a shared-use basis will greatly increase the vision research capabilities of O.U. investigators as well as Ophthalmologists, Residents and Fellows at Beaumont Hospital, who would otherwise not be able to afford the sophisticated equipment and services associated with the advanced techniques that are described.

描述（由申请人提供）：此 R24 申请的目的是增强奥克兰大学视觉研究人员的研究能力，并促进大学内视觉研究界和附属 William Beaumont 医院眼科之间的合作研究。十名研究人员，其中七名来自 O.U. 眼科研究所。三个来自 O.U.生物科学系被指定为该计划的参与者。其中六名研究人员拥有独立的 NEI 研究奖项，其中一名研究人员的一等奖申请目前正在审查中。项目包括晶状体和白内障、健康和疾病中的视网膜生物化学和代谢、细胞外基质在青光眼神经节细胞死亡中的作用、视网膜变性的光感受器相关机制以及视网膜发育中基因表达的协调。请求对细胞和分子生物学以及眼结构和成像这两个模块提供支持，以便重组和扩展 O.U. 先前持有的 NEI 核心资助的现有组织培养和电子显微镜/组织学模块。过去16年。细胞和分子生物学模块将为 O.U. 提供帮助。组织培养、基本分子技术、DNA 测序和微阵列技术领域的视觉研究人员，后者与密歇根大学凯洛格眼科中心的感觉 DNA 阵列设施相结合。眼部结构和成像模块将通过使用落射荧光、常规光学和共聚焦显微镜以及扫描和透射电子显微镜提供最先进的超微结构和成像分析功能，增强 R24 参与者的视觉研究环境。所请求的设施在共享基础上的可用性将大大提高 O.U. 的视觉研究能力。研究人员以及博蒙特医院的眼科医生、住院医师和研究员，否则他们将无法负担与所描述的先进技术相关的复杂设备和服务。

项目成果

Posterior vitreous detachment with microplasmin alters the retinal penetration of intravitreal bevacizumab (Avastin) in rabbit eyes.

• DOI: 10.1097/iae.0b013e3181e586b2
• 发表时间: 2011-02
• 期刊: Retina (Philadelphia, Pa.)
• 作者: Goldenberg DT;Giblin FJ;Cheng M;Chintala SK;Trese MT;Drenser KA;Ruby AJ
• 通讯作者: Ruby AJ

A decrease in phosphorylation of cAMP-response element-binding protein (CREBP) promotes retinal degeneration.

• DOI: 10.1016/j.exer.2011.03.017
• 发表时间: 2011-06
• 期刊: EXPERIMENTAL EYE RESEARCH
• 影响因子: 3.4
• 作者: Mali, Raghuveer S.;Zhang, Xiao M.;Chintala, Shravan K.
• 通讯作者: Chintala, Shravan K.

Expression and purification of his-tagged recombinant mouse zeta-crystallin.

• DOI: 10.1016/j.pep.2009.08.001
• 发表时间: 2010-02
• 期刊: PROTEIN EXPRESSION AND PURIFICATION
• 影响因子: 1.6
• 作者: Simpanya, Mukoma F.;Leverenz, Victor R.;Giblin, Frank J.
• 通讯作者: Giblin, Frank J.

Retinaldehyde, a potent inhibitor of gap junctional intercellular communication.

视黄醛，间隙连接细胞间通讯的有效抑制剂。

• DOI: 10.1080/15419060490471784
• 发表时间: 2004
• 期刊: Cell communication & adhesion.
• 作者: Pulukuri,Sadhona;Sitaramayya,Ari
• 通讯作者: Sitaramayya,Ari

Enzyme-induced posterior vitreous detachment in the rat produces increased lens nuclear pO2 levels.

酶诱导的大鼠后玻璃体脱离会产生透镜核PO2水平的增加。

• DOI: 10.1016/j.exer.2008.09.003
• 发表时间: 2009-02
• 期刊: EXPERIMENTAL EYE RESEARCH
• 影响因子: 3.4
• 作者: Giblin, F. J.;Quiram, P. A.;Leverenz, V. R.;Baker, R. M.;Dang, Loan;Trese, M. T.
• 通讯作者: Trese, M. T.