LTQ Orbitrap XL ETD Mass Spectrometer

描述(由申请人提供)：斯坦福大学是世界上一些最先进的生物学和医学研究的发源地。蛋白质组学作为一种研究应用，为基本的生物学问题提供了有洞察力的答案，现在是一个先决条件。蛋白质组学的基石之一，如果不是基础的话，就是质谱学。在过去五年中，随着与计算资源并行的质谱分析技术的发展，酵母蛋白质组得到了完全的鉴定和量化，人类组蛋白及其异构体的完整翻译后图谱，以及在全球蛋白质组尺度上作为对刺激的反应的信号事件(磷蛋白质组)的图谱的能力。这些深刻的发现与现代仪器的能力密不可分。该提案寻求资金帮助购买一台新的LTQ Orbitrap XL ETD质谱仪。Orbitrap将在斯坦福大学的质谱学核心设施--斯坦福大学质谱学(SUM)内进行专业操作和维护。Summ目前的一年用户群包括来自26个系的80个研究小组的245名学生、教职员工和工作人员，他们分布在医学院(60%)、人文与科学学院(30%)和工程学院(10%)。Orbitrap质谱仪的能力已经得到验证，操作仪器并将结果数据转化为具有生物学意义的结果的技术专业知识已经存在。轨道诱捕器的加入将使目前需求旺盛的研究项目变得可行。在许多其他应用中，该仪器的高质量准确性和灵敏度将使对癌细胞、干细胞和胎鼠血清的蛋白质组学定量研究成为可能。ETD的能力将被用于植物细胞信号转导和肿瘤发生相关蛋白质的磷酸化蛋白质组学研究。此外，该仪器将能够自上而下和中下而下地表征对病毒进展至关重要的蛋白质。提案中描述的七个主要使用者的研究项目与人类健康和疾病高度相关，特别是对病毒和细菌感染、干细胞和细胞命运以及几种类型的癌症的影响。这些项目代表了校园里许多研究人员面临的挑战类型。 与公共健康相关：蛋白质组学是在最广泛的范围内研究蛋白质，确定关于蛋白质的最基本信息：什么、谁、在哪里和为什么在理解疾病和疾病状态方面有意义。质谱学是已经并将继续明确回答这些问题的工具之一。将LTQ Orbitrap XL ETD质谱仪经过验证的能力应用到本提案中描述的研究中，可能会影响人类健康和疾病，推动多个领域的研究向前发展，包括病毒和细菌感染、干细胞和细胞命运以及几种类型的癌症。

DESCRIPTION (provided by applicant): Stanford University is home to some of the world's most progressive biological and medical research. Proteomics as a research application, providing insightful answers to fundamental biological questions, is now a prerequisite. One of the cornerstones of proteomics, if not the foundation, is mass spectrometry. The technological development of mass spectrometric instrumentation in parallel with computational resources over the past five years has given rise to the complete identification and quantification of the yeast proteome, complete post-translational mapping of human histones and isoforms thereof, as well as the ability to map signaling events (phosphoproteome) as a response to stimulation on a global proteomic scale. These profound findings are inextricably linked to the capabilities of modern instrumentation. This proposal seeks funds to assist in the purchase of a new LTQ Orbitrap XL ETD mass spectrometer. The Orbitrap will be professionally operated and maintained in Stanford's mass spectrometry core facility, Stanford University Mass Spectrometry (SUMS). SUMS' current 1-year user base includes 245 students, faculty, and staff in 80 research groups from 26 departments distributed across the schools of Medicine (60%), Humanities and Sciences (30%), and Engineering (10%). The capabilities of the Orbitrap mass spectrometer are proven, and the technical expertise to operate the instrumentation and translate the resulting data into biologically meaningful results is already present in SUMS. The addition of the Orbitrap would make research projects that are now in high demand viable. Amongst many other applications, the high mass accuracy and sensitivity of the instrument would enable quantitative proteomic studies on cancer cells, stem cells, and fetal mouse serum. The capabilities of ETD would be utilized in phosphoproteomic characterization of proteins involved in plant cell signaling and tumorgenesis. In addition, the instrumentation would enable top-down and middle-down characterization of proteins critical to viral progression. The research projects of seven major users, as described in the proposal, are highly relevant to human health and disease, with particular impacts on viral and bacterial infections, stem cells and cell fate, and several types of cancers. These projects are representative of the types of challenges faced by many researchers on campus. PUBLIC HEALTH RELEVANCE: Proteomics is the study of proteins on the broadest scale, determining the most basic information about proteins: the what, who, where and why have implications in understanding disease and disease states. Mass spectrometry is one tool which has addressed and will continue to answer these questions definitively. Applying the proven capabilities of the LTQ Orbitrap XL ETD mass spectrometer to the research described in this proposal has potential to impact human health and disease, moving research forward in numerous areas, including viral and bacterial infections, stem cells and cell fate, as well as several types of cancers.