Illumnia Genome Analyzer II

描述（由申请人提供）：这是一份在罗切斯特大学建立高通量DNA测序技术的提案。该大学拥有丰富的美国国立卫生研究院资助的研究组合，这些研究将受益于高通量DNA测序。从致龋细菌的基因组分析到遗传性心脏病患者长QT间期等位基因的图谱绘制，各种各样的项目将解决对人类健康有直接影响的问题。进一步的研究项目包括癌基因合作、代谢调控、信号转导、基因调控和表观遗传学。其中许多项目的pi是国际公认的各自领域的领导者，并在最具影响力的期刊上发表他们的研究成果。此外，由罗切斯特大学（University of Rochester）的科学家协调的多校区合作的纳索尼亚（一种寄生蜂）基因组计划也将从这项技术中受益匪浅。此外，具有生物信息学和生物静力学经验的数学和统计团队将开发和完善创新工具，将高通量测序技术应用于生物医学研究。所要求的仪器是Illumina基因组分析仪II。它将被安置在已建立的功能基因组学核心设施（FGC）中。FGC的专职人员将负责操作仪器。FGC的工作人员亦会协助用户处理和分析数据。更先进的计算支持将通过新落成的罗切斯特大学研究计算中心（CRC）提供，该中心拥有几个高性能计算和大型数据存储资源。CRC的工作人员包括具有基因组学专业知识的程序员和信息学家，他们将协助用户分析大规模DNA序列数据。将为该文书的运作提供机构支助和监督。将基因组核心服务扩展到高通量测序是罗彻斯特大学战略计划的一个组成部分，该计划计划在未来四年内显著扩展基因组学研究。这一战略计划包括在基因组学、系统生物学和生物信息学领域招聘10名新教员。此外，生物信息学和基因组学的本科和研究生教学也将扩大。

DESCRIPTION (provided by applicant): This is a proposal to establish high throughput DNA sequencing technology at the University of Rochester. The University has a rich portfolio of NIH-funded research that would benefit from access to high throughput DNA sequencing. Diverse projects, ranging from genome analysis in caries-causing bacteria to mapping of long QT alleles in patients with hereditary heart conditions, will address questions with direct impact on human health. Further projects include research on oncogene cooperation, metabolic regulation, signal transduction, gene regulation and epigenetics. The PIs of many of these projects are internationally recognized leaders in their fields and publish their work in the journals of the highest impact. In addition, the Nasonia (a parasitic wasp) genome project, a multi-campus effort that is coordinated by scientists at the University of Rochester would benefit substantially from this technology. Moreover, mathematical and statistical teams with experience in bioinformatics and biostatics will develop and refine innovative tools for the application of high throughput sequencing technology to biomedical research. The requested instrument is an Illumina Genome Analyzer II. It will be housed in the established functional genomics core facility (FGC). A full-time dedicated staff member of the FGC will run the instrumentation. Staff of the FGC will also provide user assistance with data processing and analysis. More advanced computational support will be available through the newly inaugurated University of Rochester Center for Research Computing (CRC), which houses several high performance computing and large data storage resources. The staff of the CRC includes programmers and informaticians with expertise in genomics, who will be available to assist users with the analysis of large scale DNA sequence data. Institutional support and oversight will be provided for the running of the instrument. The extension of genomic core services to include high throughput sequencing is an integral part of the strategic plan of the University of Rochester, which projects a significant expansion of genomics research over the coming four years. This strategic plan includes the recruitment of ten new faculty members in the areas of genomics, systems biology, and bioinformatics. In addition, undergraduate and graduate teaching in bioinformatics and genomics will be expanded.