TSQ Vantage Bundle with Acquity UltraPerformance LC

描述（由申请人提供）：由4名阿色大学医学科学（UAMS）临床研究科学家组成的主要用户组，持有7名当前NIH同行评审拨款，要求化学分离和分析系统，包括(A) Applied Biosystems (AB)/Sciex QTRAP(R) 5500 AM三重四极杆/线性离子阱质谱仪和(b) Waters Acquity UPLC(R)超高性能液相色谱仪。UAMS医学院和药学院提供基础设施支持，以全面覆盖保修后服务合同至保修后第三年，一名全职技术人员/操作员为期两年，并对现有LC-MS实验室进行轻微修改（溶剂排气管道，泵噪声降低）。用户组在他们的NIH拨款中明确需要提高色谱分辨率和增加光谱灵敏度，而不是现有的UAMS设备。他们从对现有系统的审查中得出结论，Acquity UPLC(R)- QTRAP(R) 5500 AM系统可以最好地满足这一需求，并估计由七个评审NIH奖资助的实验将占用80%的仪器时间。值得注意的是，美国国立卫生研究院资助的项目都依赖于两种核心方法中的一种或两种：利用(i) l -瓜氨酸和相关精氨酸途径底物作为肠道健康和活动的指标；（ii） l-苯丙氨酸、3-甲基- l-组氨酸和其他特定常量营养素的稳定（重）同位素，作为蛋白质合成和周转的指标。因为Drs。Wolfe和Deutz是老龄化和长寿转化研究中心（CTRAL, Donald W. Reynolds老年病学部门，UAMS）的创始人，他们共同拥有50年的专业知识来解释这些和相关代谢物测量的结果，用户组将在CTRAL的现有LC-MS/GC-MS核心中放置所要求的UPLC-QTRAP系统。Thaden博士拥有10年的生物有机分离化学家经验，5包括AB/Sciex三重四极杆质谱仪和AB/Sciex Analyst(R)软件的日常操作。瓜氨酸和氨基酸稳定同位素是强大的代谢示踪剂，能够报告健康状况，因为它适用于许多公共卫生问题。该系统将进一步了解和治疗或缓解败血症、辐射引起的肠道损伤、慢性阻塞性肺病以及与癌症、重大手术事件、高龄、饥饿等相关的脂肪酸相关底物循环异常。

DESCRIPTION (provided by applicant): A major user group of 4 University of Arkansas for Medical Sciences (UAMS) clinical research scientists, holding 7 current NIH peer-reviewed grants, request a chemical separation and analysis system comprising (a) an Applied Biosystems (AB)/Sciex QTRAP(R) 5500 AM triple Quadrupole/linear ion TRAP mass spectrometer and (b) a Waters Acquity UPLC(R) Ultra-High Performance Liquid Chromatograph. There is infrastructural support from the UAMS Colleges of Medicine and Pharmacy to fully cover post-warranty service contracts through the 3rd post-warranty year, 1 full-time technician/operator for two years, and minor modifications (solvent vent line, pump noise abatement) to an existing LC-MS laboratory. The user group documents a clear need in their NIH grants for improved chromatographic resolution and increased spectrometric sensitivity over that possible on existing UAMS equipment. They conclude from their review of available systems that the Acquity UPLC(R)- QTRAP(R) 5500 AM system can address this need best, and estimate that experiments funded by seven refereed NIH awards will occupy 80% of instrument time. Notably, the NIH-funded projects all rely on one or both of two core methodologies: utilization of (i) L-citrulline and related arginine pathway substrates as metrics of intestinal health and activity; and (ii) stable (heavy) isotopomers of L-phenylalanine, 3-methyl-L-histidine, and other select macronutrients as metrics of protein synthesis and turnover. Since Drs. Wolfe and Deutz, founders of the Center for Translational Research in Aging and Longevity (CTRAL, Donald W. Reynolds Department of Geriatrics, UAMS), jointly have >50 years expertise interpreting the results of these and related metabolite measurements, the user group would situate the requested UPLC-QTRAP system at CTRAL in its existing LC-MS/GC-MS core. That core's director Dr. Thaden has >10 years experience as a bioorganic separations chemist, 5 including day-to-day operation of AB/Sciex triple-quadrupole mass spectrometers and AB/Sciex Analyst(R) software. Citrulline and the amino-acid stable isotopes are powerful metabolic tracers, able to report on health status as it applies to a number of public health issues. The requested system would further understanding and the treatment or palliation of sepsis, radiation-induced gut damage, chronic obstructive lung disease, and fatty acid related substrate-cycling aberrancies associated with cancer, major surgical events, advanced aging, starvation, etc. PUBLIC HEALTH RELEVANCE: Advances in our understanding and treatment of 4 seemingly disparate public health problems share a heavy dependence on instruments able to measure traces of certain research molecules - citrulline, and a family of stable, safe isotopes - in blood, urine and tissue. The health problems, topics of 7 NIH-funded projects at the University of Arkansas for Medical Science (UAMS), are severe damage to the intestines after radiation exposure; sepsis (blood infection) coming from major infection; muscle wasting accompanying chronic obstructive pulmonary disease (COPD); and disturbed fatty acid and protein metabolism associated with a spectrum of conditions, for instance, cancer, advanced aging, enforced inactivity, and major surgical events. The requested QTRAP(r) 5500 AM Mass Spectrometer and Acquity UPLC(r) chromatograph system would appreciably speed progress in these research efforts on sepsis, radioprotective agents, COPD and metabolic substrate cycling here in Central Arkansas.