ESSENTIAL AMINO ACIDS AND RESISTANCE EXERCISE IN AGING

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 肌肉质量的丧失会导致全身虚弱、行动不便和身体依赖，并可能导致对压力的反应受损，这与感染、手术和创伤期间较高的死亡率有关。 氨基酸和举重都可以增加肌肉的尺寸。研究表明，与年轻志愿者相比，老年人运动后肌肉生长较少。然而，还没有研究检验氨基酸和抗阻运动的联合影响。 我们假设，在一轮举重运动后摄入氨基酸将改善老年男性的短期肌肉生长。 其具体目的是确定在举重运动后一小时摄入20克必需氨基酸是否会像年轻人一样刺激老年男性的短期肌肉生长。 将招募6名健康的年轻和老年男性志愿者。在锻炼一小时后，每个受试者将获得20克必需氨基酸。然后我们将评估血液氨基酸含量的变化，以及血液样本中胰岛素和睾酮等激素的变化。每个肌肉样本将被用来测量肌肉蛋白质合成，并评估控制蛋白质合成的细胞信号通路的变化。 这些研究将提供对肌肉为什么会随着年龄的增长而减少的洞察，然后将帮助我们开发基于科学的干预措施来改善肌肉大小。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The loss of muscle mass leads to overall weakness, immobility and physical dependence, and can be responsible for an impaired response to stress, which is associated with higher mortality rates during infection, surgery, and trauma. Amino acids and weight lifting can both increase muscle size. It has been shown that muscle growth post-exercise is less in the elderly as compared to young volunteers. However, there have been no studies that have examined the combined effect of amino acids and resistance exercise. We hypothesize that amino acid ingestion following a bout of weight lifting exercise will improve short-term muscle growth in older men. The specific aims are to determine if 20 grams of essential amino acids ingested one hour following a bout of weight lifting exercise will stimulate short-term muscle growth in older men to the same extent as seen in the young. Six healthy young and older male volunteers will be enrolled. One hour following the exercise session each subject will receive 20 grams of essential amino acids. We will then assess changes in blood amino acid content, hormone changes such as insulin and testosterone from the blood samples. Each muscle sample will be used to measure muscle protein synthesis and to assess changes in cell signaling pathways the control protein synthesis. These studies will provide insight into why muscle loss is occurring with age and will then help us to develop scientifically-based interventions for improving muscle size.