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LTQ-Orbitrap-XL-ETD Mass Spectrometer for LLU Mass Spectrometry Facility

用于 LLU 质谱设备的 LTQ-Orbitrap-XL-ETD 质谱仪

基本信息

批准号：
7795579
负责人：
Kangling Zhang
金额：
$ 50万
依托单位：
LOMA LINDA UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-04-22 至 2011-04-21
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): Summary This application requests funding for a state-of-the-art shared mass spectrometer that will provide collaborative research for the Loma Linda University mass spectrometry facility. The LTQ-Orbitrap-XL-ETD mass spectrometer is manufactured by Thermo Fisher Scientific. The LTQ-Orbitrap-XL-ETD LC/MS/MS system provides understanding sensitivity, resolving power, accuracy of mass measurement, and wide dynamic range characteristics. It combines three different and complementary fragmentation techniques CID, HCD, and ETD with the proven benefits of Orbitrap technology for most comprehensive solution for complex PTM analysis, intelligent sequencing of peptides, top-down and middle-down analysis, and protein quantification via label-free or stable isotope labeling such as iTRAQ and TMT. As the cost of purchasing this mass spectrometer is $729,000, we request $499,999.23 from NIH shared instrumentation program and the School of Medicine of Loma Linda University will commit $229,000.77. This mass spectrometer will support 11 major NIH users in nine departments or centers at Loma Linda University and 2 major users from nearby institutes, Dr. Michael Grunstein at UCLA and Dr. Jian-Kang Zhu at UCR, who will collaborate with our facility to pursue their research in epigenetics. More than 30 investigators (major users and minor users), majority of whom holds NIH grants, will be benefited from this instrument. With acquisition of this instrument our major NIH users' research will be significantly advanced in the areas including identification of biomarkers in disease and cancer, neuron-injury and neonatal pain, understanding the mechanisms involved in DNA damage and repair, pathology of infectious disease, and histone modifications in epigenetic and cancer studies. PUBLIC HEALTH RELEVANCE: We request a LTQ-Orbitrap-XL-ETD mass spectrometer to meet the needs of our NIH sponsored users to advance their research in epigenetics, infectious disease, neuron injury, pain, hypoxia stress and human cancers, etc.

描述（由申请人提供）：摘要本申请请求资助最先进的共享质谱仪，该质谱仪将为洛马琳达大学质谱设施提供合作研究。 LTQ-Orbitrap-XL-ETD 质谱仪由 Thermo Fisher Scientific 制造。 LTQ-Orbitrap-XL-ETD LC/MS/MS 系统提供了了解灵敏度、分辨率、质量测量精度和宽动态范围特性。它将三种不同且互补的片段化技术 CID、HCD 和 ETD 与 Orbitrap 技术经过验证的优势相结合，为复杂 PTM 分析、肽智能测序、自上而下和中下分析以及通过无标记或稳定同位素标记（如 iTRAQ 和 TMT）进行蛋白质定量提供最全面的解决方案。由于购买该质谱仪的成本为 729,000 美元，我们向 NIH 共享仪器计划请求 499,999.23 美元，洛马琳达大学医学院将承诺 229,000.77 美元。该质谱仪将为洛马琳达大学 9 个系或中心的 11 位 NIH 主要用户以及附近研究所的 2 位主要用户（加州大学洛杉矶分校的 Michael Grunstein 博士和加州大学河滨分校的朱建康博士）提供支持，他们将与我们的设施合作开展表观遗传学研究。超过 30 名研究人员（主要用户和次要用户）将从该工具中受益，其中大多数人拥有 NIH 资助。购买该仪器后，我们 NIH 主要用户的研究将在以下领域取得显着进展：疾病和癌症、神经元损伤和新生儿疼痛的生物标志物鉴定、了解 DNA 损伤和修复所涉及的机制、传染病的病理学以及表观遗传学和癌症研究中的组蛋白修饰。公共健康相关性：我们请求使用 LTQ-Orbitrap-XL-ETD 质谱仪来满足 NIH 资助的用户的需求，以推进他们在表观遗传学、传染病、神经元损伤、疼痛、缺氧应激和人类癌症等方面的研究。