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LTQ-Orbitrap-XL-ETD Mass Spectrometer for LLU Mass Spectrometry Facility

用于 LLU 质谱设备的 LTQ-Orbitrap-XL-ETD 质谱仪

基本信息

批准号：
7795579
负责人：
Kangling Zhang
金额：
$ 50万
依托单位：
LOMA LINDA UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-04-22 至 2011-04-21
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): Summary This application requests funding for a state-of-the-art shared mass spectrometer that will provide collaborative research for the Loma Linda University mass spectrometry facility. The LTQ-Orbitrap-XL-ETD mass spectrometer is manufactured by Thermo Fisher Scientific. The LTQ-Orbitrap-XL-ETD LC/MS/MS system provides understanding sensitivity, resolving power, accuracy of mass measurement, and wide dynamic range characteristics. It combines three different and complementary fragmentation techniques CID, HCD, and ETD with the proven benefits of Orbitrap technology for most comprehensive solution for complex PTM analysis, intelligent sequencing of peptides, top-down and middle-down analysis, and protein quantification via label-free or stable isotope labeling such as iTRAQ and TMT. As the cost of purchasing this mass spectrometer is $729,000, we request $499,999.23 from NIH shared instrumentation program and the School of Medicine of Loma Linda University will commit $229,000.77. This mass spectrometer will support 11 major NIH users in nine departments or centers at Loma Linda University and 2 major users from nearby institutes, Dr. Michael Grunstein at UCLA and Dr. Jian-Kang Zhu at UCR, who will collaborate with our facility to pursue their research in epigenetics. More than 30 investigators (major users and minor users), majority of whom holds NIH grants, will be benefited from this instrument. With acquisition of this instrument our major NIH users' research will be significantly advanced in the areas including identification of biomarkers in disease and cancer, neuron-injury and neonatal pain, understanding the mechanisms involved in DNA damage and repair, pathology of infectious disease, and histone modifications in epigenetic and cancer studies. PUBLIC HEALTH RELEVANCE: We request a LTQ-Orbitrap-XL-ETD mass spectrometer to meet the needs of our NIH sponsored users to advance their research in epigenetics, infectious disease, neuron injury, pain, hypoxia stress and human cancers, etc.

描述（由申请人提供）：摘要本申请要求资助一个国家的最先进的共享质谱仪，将提供合作研究的洛马琳达大学质谱设施。LTQ-Orbitrap-XL-ETD质谱仪由Thermo Fisher Scientific生产。LTQ-Orbitrap-XL-ETD LC/MS/MS系统具有灵敏度、分辨率、质量测量准确度和宽动态范围特性。它将CID、HCD和ETD三种不同的互补片段化技术与Orbitrap技术的成熟优势相结合，为复杂的PTM分析、肽的智能测序、自上而下和自下而上的分析以及通过无标记或稳定同位素标记（如iTRAQ和TMT）进行的蛋白质定量提供了最全面的解决方案。由于购买该质谱仪的成本为729，000美元，我们向NIH共享仪器计划申请499，999.23美元，洛马琳达大学医学院将承诺229，000.77美元。该质谱仪将支持洛马琳达大学9个部门或中心的11个主要NIH用户和附近研究所的2个主要用户，加州大学洛杉矶分校的Michael Grunstein博士和加州大学河滨分校的Jian-Kang Zhu博士，他们将与我们的设施合作，继续他们在表观遗传学方面的研究。30多名研究人员（主要用户和次要用户），其中大多数持有美国国立卫生研究院的赠款，将受益于这一工具。通过收购该仪器，我们的主要NIH用户的研究将在以下领域取得显著进展：疾病和癌症的生物标志物的鉴定，神经元损伤和新生儿疼痛，了解DNA损伤和修复的机制，感染性疾病的病理学以及表观遗传和癌症研究中的组蛋白修饰。公共卫生相关性：我们要求LTQ-Orbitrap-XL-ETD质谱仪，以满足我们NIH赞助用户的需求，以推进他们在表观遗传学，传染病，神经元损伤，疼痛，缺氧应激和人类癌症等方面的研究。