A Janus Robotic Multi-Assay System for Quantitative Biomarker Research

用于定量生物标志物研究的 Janus 机器人多重测定系统

• 批准号: 7795635
• 负责人: DANIEL T STEIN
• 金额: $ 24.96万
• 依托单位: MONTEFIORE MEDICAL CENTER (BRONX, NY)
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-22 至 2011-04-21
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7795635
• 关键词: Accounting; Address; Automation; Basic Science; Biological Assay; Biological Markers; Clinical; Clinical Research; Communities; DNA; Data; Detection; Enzyme-Linked Immunosorbent Assay; Equipment; Funding; Future; Gamma counter; Goals; Housing; Human; Immunoassay; Information Systems; Institutes; Isotopes; Laboratories; Link; Liquid substance; Mass Spectrum Analysis; Measurement; Medical center; Methods; Minor; Preparation; Procedures; Productivity; Reader; Request for Proposals; Research; Research Personnel; Robot; Robotics; Sampling; Scientist; Services; System; Translational Research; United States National Institutes of Health; cost; data management; design; detector; improved; liquid chromatography mass spectrometry; meetings; novel; two-dimensional

DESCRIPTION (provided by applicant): This proposal requests funds for the purchase of an integrated Perkin Elmer Janus Robotic Liquid Handler Multi-assay Detection and Data Management System, to be housed within a CTSA-sponsored core at the Einstein-Montefiore institute for Clinical and Translational Research (ICTR). The liquid handler system is linked directly to a Perkin Elmer AlphaLISA reader and Wizard2 gamma counter to provide for ELISA, AlphaLISA and radiometric analysis. The system will also provide front end barcode identification and sample preparation for quantitative mass spectrometry performed on our ultrahigh sensitivity 2 dimensional Agilent/ABI4000 LC/MS system. The integrated robotic, immuno-, radiometric assay and existing LC/MS equipment will be located in the Biomarker Analytical Core Laboratory of the ICTR. The Biomarker Analytical Core Laboratory provides high quality immunoassay, and quantitative mass spectrometry biomarker measurements to the basic science, translational and clinical research communities within the Medical Center as well as through collaborative projects. Biomarker research has exploded, and current capacity of the combined Biomarker Analytical Core Lab is unable to meet current and projected future demand. This system will address this critical bottleneck by providing high throughput and novel assay services to NIH funded investigators, as well as providing additional capabilities to enable efficient use of existing mass spectrometry equipment. It is the goal of the Biomarker Analytical Core Lab to automate data handling to improve efficiencies, minimize human error, facilitate data transfer to investigators, and to improve internal cost accounting. Although there are multiple liquid handling robots within the Medical Center, they are not suitable or available to the automation tasks outlined here. Liquid handling robots are designed into integrated systems that automate one or at most a very few repetitive tasks. No robots are currently available for integration into a medium high throughput analytical system that can support ELISA, AlphaLISA, RIA and isotope dilution mass spectrometry assay methods. In addition, current Biomarker Analytical Core Lab assay detection methods rely on outmoded ELISA and gamma detectors that are not capable of being integrated into an automated data system resulting in major inefficiencies. The proposed Janus robotic liquid handler AlphaPLUS/Wizard2 detection system will allow an increase in throughput of >200 samples per day; this productivity will be supplemented by the additional capability for preparation of samples prior to isotope dilution assay LC/MS analysis as well as DNA extraction. All of these procedures are labor intensive and can be greatly accelerated by partial or full automation. The purchase of this integrated system will significantly promote the research objectives of the 9 scientists in the major user group and many more minor users.

描述（由申请人提供）：该提案要求提供资金，用于购买集成的Perkin Elmer Janus机器人液体检测器多检测和数据管理系统，该系统将安装在Einstein-Montefiore临床和转化研究所（ICTR）的CTSA赞助的核心内。液体处理器系统直接连接到Perkin Elmer AlphaLISA读数器和Wizard 2 γ计数器，以提供ELISA、AlphaLISA和放射性分析。该系统还将提供前端条形码识别和样品制备，用于在我们的高灵敏度二维Agilent/ABI 4000 LC/MS系统上进行定量质谱分析。机器人、免疫、放射测定和现有的液相色谱/质谱仪综合设备将设在卢旺达问题国际法庭生物标志物分析核心实验室。生物标志物分析核心实验室为医学中心内的基础科学，转化和临床研究社区以及通过合作项目提供高质量的免疫测定和定量质谱生物标志物测量。生物标志物研究已经爆炸式增长，合并后的生物标志物分析核心实验室的现有能力无法满足当前和预计的未来需求。该系统将通过为NIH资助的研究人员提供高通量和新颖的分析服务来解决这一关键瓶颈，并提供额外的功能以有效利用现有的质谱设备。生物标志物分析核心实验室的目标是自动化数据处理，以提高效率，最大限度地减少人为错误，促进数据传输给研究者，并改善内部成本核算。虽然医疗中心内有多个液体处理机器人，但它们不适合或不适用于此处概述的自动化任务。液体处理机器人被设计成集成系统，自动化一个或最多很少的重复性任务。目前没有机器人可用于集成到可以支持ELISA、AlphaLISA、RIA和同位素稀释质谱分析方法的中高通量分析系统中。此外，目前的生物标志物分析核心实验室测定检测方法依赖于过时的ELISA和γ检测器，这些检测器不能集成到自动化数据系统中，导致效率低下。拟议的Janus机器人液体处理器AlphaPLUS/Wizard 2检测系统将允许每天增加>200个样品的吞吐量;这种生产率将通过在同位素稀释测定LC/MS分析以及DNA提取之前制备样品的额外能力来补充。所有这些过程都是劳动密集型的，并且可以通过部分或全部自动化来大大加速。购买这一综合系统将大大促进主要用户群中的9名科学家和更多的次要用户的研究目标。