Maldi/ToF/ToF Mass Spectrometer for the University of Utah

描述（由申请人提供）：本提案是一项资金申请，用于购买Maldi/ToF/ToF质谱仪（Ultraflex-III，Bruker Daltonics），以升级犹他州大学的质谱和蛋白质组学核心设施。该仪器将用于两个主要目的：1）更换旧的低性能Maldi/ToF仪器; 2）通过Maldi/ToF/ToF设计提供显著的性能升级和新的MS/MS功能。来自犹他州大学的12个主要用户和28个活跃的NIH资助项目已被确定为当前和未来的项目，以确保立即和持续的全容量长期使用。大约有35到40个额外的小用户。主要用户组将利用Maldi/ToF/ToF仪器的不同功能。几个主要用户将需要MS/MS能力用于他们的研究项目，而许多主要用户将受益于高性能和高灵敏度，用于分析大生物分子，如聚合物和完整蛋白质。具有低能量LID片段化或高能量CID片段化的MS/MS将用于不同的应用。例如，一些用户需要高能CID来研究硫酸化二糖和蛋白聚糖分子中的硫酸化模式;其他用户需要LID片段化来表征修饰肽的复杂结构。所要求的Maldi/ToF/ToF仪器的研究应用广泛而多样，包括药物输送系统的聚合物调查，完整的受体蛋白，荧光肽探针的开发，发现新的神经肽从有毒蜗牛，硫酸化模式在蛋白聚糖中的作用，以及发现未知的蛋白质金属/配体复合物等主题。Maldi/ToF/ToF仪器将安装在MS核心设施中，这是一个中央质谱共享资源，已在犹他州大学运行近30年。MS核心由该机构在结构良好的核心研究设施框架内管理，包括监督和咨询委员会。有非常强大的机构的财政支持，长期使用的仪器，包括服务协议和全职博士质谱师。对犹他州大学和区域机构的长期影响是为科学和医学研究界带来技术先进的资源，这些资源将在多年内提供最先进的质谱分析，并将刺激新的研究项目。Ultraflex-III Maldi/ToF/ToF仪器的分析能力将在加速许多领域的研究方面发挥重要作用，特别是基于聚合物的药物递送系统和许多小分子结构表征项目。 公共卫生相关性：所要求的Maldi/ToF/ToF仪器是一种高性能质谱仪，将为犹他州大学的质谱和蛋白质组学核心设施（大学共享资源）的质谱能力提供重大升级。该仪器将取代过时的Maldi/ToF质谱仪，并带来强大的技术，以推进犹他州大学和区域机构的100多名NIH资助的研究人员的研究。这种最先进的质谱仪将对犹他州大学产生长期影响，为科学和医学界带来技术先进的资源，这将有助于研究人员在以下领域取得新的发现并激发创新的研究思路：通过生物聚合物研究药物输送系统和发现有毒蜗牛中新型神经肽的新医学用途。该质谱仪将在未来许多年内在加速研究和开发广泛的生物医学应用方面发挥重要作用。

DESCRIPTION (provided by applicant): This proposal is a request for funds to purchase a Maldi/ToF/ToF mass spectrometer (Ultraflex-III, Bruker Daltonics) to upgrade the Mass Spectrometry and Proteomics Core Facility at the University of Utah. The instrument will serve two primary purposes: 1) replacement of an old, low-performance Maldi/ToF instrument and 2) provide a significant performance upgrade and new MS/MS capabilities with the Maldi/ToF/ToF design. Twelve major users from the University of Utah with 28 active NIH-funded projects have been identified with current and future projects that ensure immediate and continued full-capacity long-term usage. There are about 35 to 40 additional minor users. The group of major users will utilize different capabilities of the Maldi/ToF/ToF instrument. Several major users will require MS/MS capability for their research projects, while many of the major users will benefit from high performance and high sensitivity for analysis of large biomolecules, such as polymers and intact proteins. MS/MS with either low-energy LID fragmentation or high- energy CID fragmentation will be utilized for different applications. For instance, some users need high-energy CID for studying sulfated disaccharides and sulfation patterns in proteoglycan molecules; other users require LID fragmentation to characterize complex structures of modified peptides. Research applications for the requested Maldi/ToF/ToF instrument are wide and diverse, including topics such as polymer investigations for drug delivery systems, intact receptor proteins, development of fluorogenic peptide probes, discovery of novel neuropeptides from venomous snails, the role of sulfation patterns in proteoglycans, and discovery of unknown protein metal/ligand complexes. The Maldi/ToF/ToF instrument will be housed in the MS Core Facility, a central mass spectrometry shared resource that has been in operation at the University of Utah for nearly 30 years. The MS Core is administered by the Institution within a well-structured Core Research Facilities framework, including Oversight and Advisory committees. There is very strong Institutional financial support for long-term utilization of the instrument, including service agreements and full-time PhD mass spectrometrists. The long-term impact to the University of Utah and regional institutions is bringing a technologically-advanced resource to the scientific and medical research community that will provide state-of-the-art mass spectrometry for many years and will stimulate new research projects. The analytical power of the Ultraflex-III Maldi/ToF/ToF instrument will play a major role in accelerating research in many areas, particularly polymer- based drug delivery systems and many small molecule structure characterization projects. PUBLIC HEALTH RELEVANCE: The requested Maldi/ToF/ToF instrument is a high-performance mass spectrometer that will provide a significant upgrade to the mass spectrometry capabilities of the Mass Spectrometry and Proteomics Core Facility at the University of Utah, a University shared resource. The instrument will replace an obsolete Maldi/ToF mass spectrometer and bring powerful technology to advance the research of potentially more than 100 NIH-funded investigators at the University of Utah and regional institutions. This state-of-the-art mass spectrometer will have a long-term impact to the University of Utah by bringing a technologically advanced resource to the scientific and medical community which will help investigators make new discoveries and stimulate innovate research ideas in areas such as: drug delivery systems through biopolymer research and discovery of new medical uses for novel neuropeptides from venomous snails. This mass spectrometer will play a major role in accelerating research and development for a broad range of biomedical applications for many years to come.