Maldi/ToF/ToF Mass Spectrometer for the University of Utah

犹他大学 Maldi/ToF/ToF 质谱仪

• 批准号: 7795990
• 负责人: CHAD C NELSON
• 金额: $ 48.99万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7795990
• 关键词: Advisory Committees; Agreement; Area; Arts; Biopolymers; Communities; Complex; Core Facility; Development; Disaccharides; Doctor of Philosophy; Drug Delivery Systems; Ensure; Financial Support; Funding; Future; Housing; Inorganic Sulfates; Institution; Investigation; Ligands; Mass Spectrum Analysis; Medical; Medical Research; Metals; Minor; Neuropeptides; Pattern; Peptides; Performance; Play; Polymers; Proteins; Proteoglycan; Proteomics; Request for Applications; Research; Research Personnel; Research Project Grants; Resource Sharing; Resources; Role; Services; Snails; Structure; Technology; Time; Universities; Unspecified or Sulfate Ion Sulfates; Utah; base; design; innovation; instrument; mass spectrometer; mass spectrometry shared resource; novel; operation; public health relevance; receptor; research and development; research facility; small molecule; sulfation

DESCRIPTION (provided by applicant): This proposal is a request for funds to purchase a Maldi/ToF/ToF mass spectrometer (Ultraflex-III, Bruker Daltonics) to upgrade the Mass Spectrometry and Proteomics Core Facility at the University of Utah. The instrument will serve two primary purposes: 1) replacement of an old, low-performance Maldi/ToF instrument and 2) provide a significant performance upgrade and new MS/MS capabilities with the Maldi/ToF/ToF design. Twelve major users from the University of Utah with 28 active NIH-funded projects have been identified with current and future projects that ensure immediate and continued full-capacity long-term usage. There are about 35 to 40 additional minor users. The group of major users will utilize different capabilities of the Maldi/ToF/ToF instrument. Several major users will require MS/MS capability for their research projects, while many of the major users will benefit from high performance and high sensitivity for analysis of large biomolecules, such as polymers and intact proteins. MS/MS with either low-energy LID fragmentation or high- energy CID fragmentation will be utilized for different applications. For instance, some users need high-energy CID for studying sulfated disaccharides and sulfation patterns in proteoglycan molecules; other users require LID fragmentation to characterize complex structures of modified peptides. Research applications for the requested Maldi/ToF/ToF instrument are wide and diverse, including topics such as polymer investigations for drug delivery systems, intact receptor proteins, development of fluorogenic peptide probes, discovery of novel neuropeptides from venomous snails, the role of sulfation patterns in proteoglycans, and discovery of unknown protein metal/ligand complexes. The Maldi/ToF/ToF instrument will be housed in the MS Core Facility, a central mass spectrometry shared resource that has been in operation at the University of Utah for nearly 30 years. The MS Core is administered by the Institution within a well-structured Core Research Facilities framework, including Oversight and Advisory committees. There is very strong Institutional financial support for long-term utilization of the instrument, including service agreements and full-time PhD mass spectrometrists. The long-term impact to the University of Utah and regional institutions is bringing a technologically-advanced resource to the scientific and medical research community that will provide state-of-the-art mass spectrometry for many years and will stimulate new research projects. The analytical power of the Ultraflex-III Maldi/ToF/ToF instrument will play a major role in accelerating research in many areas, particularly polymer- based drug delivery systems and many small molecule structure characterization projects. PUBLIC HEALTH RELEVANCE: The requested Maldi/ToF/ToF instrument is a high-performance mass spectrometer that will provide a significant upgrade to the mass spectrometry capabilities of the Mass Spectrometry and Proteomics Core Facility at the University of Utah, a University shared resource. The instrument will replace an obsolete Maldi/ToF mass spectrometer and bring powerful technology to advance the research of potentially more than 100 NIH-funded investigators at the University of Utah and regional institutions. This state-of-the-art mass spectrometer will have a long-term impact to the University of Utah by bringing a technologically advanced resource to the scientific and medical community which will help investigators make new discoveries and stimulate innovate research ideas in areas such as: drug delivery systems through biopolymer research and discovery of new medical uses for novel neuropeptides from venomous snails. This mass spectrometer will play a major role in accelerating research and development for a broad range of biomedical applications for many years to come.

描述(由申请人提供)：本提案申请资金购买MALDI/ToF/ToF质谱仪(Ultraflex-III，Bruker Daltonics)，以升级犹他大学的质谱学和蛋白质组学核心设施。该仪器将用于两个主要目的：1)替换旧的、低性能的MALDI/ToF仪器；2)通过MALDI/ToF/ToF设计提供显著的性能升级和新的MS/MS功能。来自犹他大学的12个主要用户和28个由国家卫生研究院资助的正在进行的项目已被确定为当前和未来的项目，以确保立即和持续的满负荷长期使用。还有大约35到40个额外的次要用户。主要用户组将利用MALDI/TOF/TOF仪器的不同能力。几个主要用户的研究项目将需要MS/MS能力，而许多主要用户将受益于高性能和高灵敏度，用于分析大的生物分子，如聚合物和完整蛋白质。具有低能量LID碎片或高能CID碎片的MS/MS将用于不同的应用。例如，一些用户需要高能CID来研究蛋白多糖分子中的硫化双糖和硫化模式；另一些用户需要LID碎裂来表征修饰多肽的复杂结构。所需的MALDI/ToF/ToF仪器的研究应用广泛而多样，包括用于药物递送系统的聚合物研究、完整的受体蛋白、荧光肽探针的开发、从有毒蜗牛中发现新的神经肽、蛋白多糖中硫酸盐化模式的作用以及未知蛋白质金属/配体络合物的发现。MALDI/ToF/ToF仪器将安装在MS核心设备中，这是犹他大学运行了近30年的中央质谱学共享资源。MS核心由研究所在一个结构良好的核心研究设施框架内进行管理，包括监督和咨询委员会。该仪器的长期使用有非常强大的机构资金支持，包括服务协议和全职博士质谱师。对犹他大学和地区机构的长期影响正在为科学和医学研究界带来技术先进的资源，这将提供多年最先进的质谱学，并将刺激新的研究项目。Ultraflex-III MALDI/ToF/ToF仪器的分析能力将在加速许多领域的研究方面发挥重要作用，特别是基于聚合物的药物输送系统和许多小分子结构表征项目。 与公众健康相关：所要求的MALDI/ToF/ToF仪器是一种高性能质谱仪，它将对犹他大学的质谱学和蛋白质组学核心设施的质谱学能力进行重大升级，该大学共享资源。该仪器将取代过时的MALDI/ToF质谱仪，并带来强大的技术，以推进犹他大学和地区机构可能由NIH资助的100多名研究人员的研究。这一最先进的质谱仪将对犹他大学产生长期影响，它将为科学和医学界带来技术先进的资源，帮助研究人员在以下领域做出新发现并激发创新研究想法：通过生物聚合物研究药物输送系统，以及从有毒蜗牛中发现新的神经肽的新医疗用途。在未来的许多年里，这种质谱仪将在加速广泛的生物医学应用的研究和开发方面发挥重要作用。