Mass Spectrometry and Proteomics Shared Resouce

质谱和蛋白质组学（MSP）共享资源为亨斯迈癌症研究所（HCI）成员和整个犹他州大学研究社区提供广泛的质谱服务，包括最先进的仪器、咨询和专业知识，特别是针对基于蛋白质组学的研究。混合傅里叶变换质谱仪（型号LTQ-FT，ThermoElectron）是主要的蛋白质组学仪器。它提供低至50阿托摩尔水平的蛋白质鉴定（ID），高质量准确度（<2 ppm），以及通过电子捕获解离（ECD），红外多光子解离（IRMPD）和碰撞诱导解离（CID）进行肽测序。蛋白质组学服务包括蛋白质ID;翻译后修饰的表征（例如，磷酸化）;和完整蛋白质的分析。该资源还为广泛的其他研究应用提供质谱服务，如天然产物、药物和药物-聚合物缀合物、核酸、DNA扩增产物（例如，PCR）、大生物分子和聚合物、肽和小合成分子。乍得纳尔逊，博士，自2003年以来一直指导MSP共享资源，与两名工作人员的协助。MSP共享资源的仪器和工作人员位于中心位置。MSP共享资源服务于所有基于HCI实验室的科学计划（细胞生长和分化的核控制;细胞反应和调节;成像，诊断和治疗;和胃肠道癌症），表征分子，特别是蛋白质和翻译后修饰，在癌症机制中发挥作用。此外，MSP共享资源对小化合物的表征，包括天然产物和药物递送聚合物，对HCI的开发治疗活性做出了重大贡献。MSP共享资源是一个机构管理的设施，由大学和HCI领导监督。有一个学院咨询委员会，并通过年度调查审查资源的用户满意度。该设施以按服务收费的方式运营（按存储容量计费）。2008年，癌症中心成员通过同行评审资金和癌症中心共享资源对MSP共享资源的总体使用率为31%。请CCSG提供资金，以支付拟议资源预算的14%（44，857美元）。MSP共享资源有足够的容量供癌症中心成员额外使用。

The Mass Spectrometry and Proteomics (MSP) Shared Resource provides Huntsman Cancer Institute (HCI) members and the entire University of Utah research community a broad range of mass spectrometry services, including state-of-the-art instrumentation, consultation, and expertise, particularly for proteomics-based research. The hybrid Fourier-Transform mass spectrometer (model LTQ-FT, ThermoElectron) is the main proteomics instrument. It provides protein identification (ID) at levels as low as 50 attomole, high-mass accuracy (<2ppm), and peptide sequencing by electron-capture dissociation (ECD), infrared multiphoton dissociation (IRMPD), and collision-induced dissociation (CID). Proteomics services include protein ID; characterization of post-translational modifications (e.g., phosphorylafion); and analysis of intact proteins. The Resource also provides mass spectrometry services for a wide range of other research applications such as structural analysis of natural products, drugs and drug-polymer conjugates, nucleic acids, DNA amplification products (e.g., PCR), large biomolecules and polymers, peptides, and small synthetic molecules. Chad Nelson, PhD, has directed the MSP Shared Resource since 2003, with the assistance of two staff members. The instrumentation and staff of the MSP Shared Resource are centrally located. The MSP Shared Resource serves all HCI laboratory-based scientific Programs (Nuclear Control of Cell Growth and Differentiation; Cell Response and Regulation; Imaging, Diagnostics, and Therapeutics; and Gastrointestinal Cancers), characterizing molecules, particularly proteins and post-translational modifications, that play roles in cancer mechanisms. In addition, characterization of small compounds by the MSP Shared Resource, including natural products and drug-delivery polymers, contributes significantly to the developmental therapeutics activities of HCI. The MSP Shared Resource is an institutionally managed facility with supervision from both University and HCI leadership. There is a Faculty Advisory Committee and the Resource is reviewed for user satisfaction by annual surveys. The facility is operated on a fee-for-service basis (chargeback). In 2008, overall use of the MSP Shared Resource by Cancer Center members with peer-reviewed funding and Cancer Center Shared Resources was 31 percent. Funds are requested from the CCSG to cover 14 percent ($44,857) of the proposed Resource budget. The MSP Shared Resource has ample capacity for additional use by Cancer Center members.