Mass Spectrometry and Proteomics Shared Resouce

质谱和蛋白质组学共享资源

• 批准号: 8465119
• 负责人: CHAD C NELSON
• 金额: $ 4.77万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8465119
• 关键词: Advisory Committees; Budgets; Cancer Center; Cancer Center Support Grant; Cell Differentiation process; Cells; Chad; Communities; Consultations; DNA amplification; Developmental Therapeutics Program; Diagnostic Imaging; Dissociation; Doctor of Philosophy; Drug Delivery Systems; Electrons; Equipment; Faculty; Fee-for-Service Plans; Fourier Transform; Funding; Goals; Hybrids; Institutes; Laboratories; Leadership; Malignant Neoplasms; Malignant neoplasm of gastrointestinal tract; Mass Spectrum Analysis; Modeling; NCI Center for Cancer Research; Natural Product Drug; Nuclear; Nucleic Acids; Peer Review; Peptides; Pharmaceutical Preparations; Play; Polymers; Post-Translational Protein Processing; Proteins; Proteomics; Proteomics Shared Resource; Regulation; Research; Resource Sharing; Resources; Role; Services; Structure; Supervision; Surveys; Therapeutic; Training; Universities; Utah; base; cell growth; instrument; instrumentation; mass spectrometer; member; programs; protein aminoacid sequence; response; satisfaction

The Mass Spectrometry and Proteomics (MSP) Shared Resource provides Huntsman Cancer Institute (HCI) members and the entire University of Utah research community a broad range of mass spectrometry services, including state-of-the-art instrumentation, consultation, and expertise, particularly for proteomics-based research. The hybrid Fourier-Transform mass spectrometer (model LTQ-FT, ThermoElectron) is the main proteomics instrument. It provides protein identification (ID) at levels as low as 50 attomole, high-mass accuracy (<2ppm), and peptide sequencing by electron-capture dissociation (ECD), infrared multiphoton dissociation (IRMPD), and collision-induced dissociation (CID). Proteomics services include protein ID; characterization of post-translational modifications (e.g., phosphorylafion); and analysis of intact proteins. The Resource also provides mass spectrometry services for a wide range of other research applications such as structural analysis of natural products, drugs and drug-polymer conjugates, nucleic acids, DNA amplification products (e.g., PCR), large biomolecules and polymers, peptides, and small synthetic molecules. Chad Nelson, PhD, has directed the MSP Shared Resource since 2003, with the assistance of two staff members. The instrumentation and staff of the MSP Shared Resource are centrally located. The MSP Shared Resource serves all HCI laboratory-based scientific Programs (Nuclear Control of Cell Growth and Differentiation; Cell Response and Regulation; Imaging, Diagnostics, and Therapeutics; and Gastrointestinal Cancers), characterizing molecules, particularly proteins and post-translational modifications, that play roles in cancer mechanisms. In addition, characterization of small compounds by the MSP Shared Resource, including natural products and drug-delivery polymers, contributes significantly to the developmental therapeutics activities of HCI. The MSP Shared Resource is an institutionally managed facility with supervision from both University and HCI leadership. There is a Faculty Advisory Committee and the Resource is reviewed for user satisfaction by annual surveys. The facility is operated on a fee-for-service basis (chargeback). In 2008, overall use of the MSP Shared Resource by Cancer Center members with peer-reviewed funding and Cancer Center Shared Resources was 31 percent. Funds are requested from the CCSG to cover 14 percent ($44,857) of the proposed Resource budget. The MSP Shared Resource has ample capacity for additional use by Cancer Center members.

质谱学和蛋白质组学(MSP)共享资源为亨斯迈癌症研究所(HCI)成员和整个犹他大学研究界提供广泛的质谱学服务，包括最先进的仪器、咨询和专业知识，特别是基于蛋白质组的研究。混合傅立叶变换质谱仪(LTQ-FT型，ThermoElectron)是蛋白质组学的主要仪器。它提供低至50attomol的蛋白质识别(ID)、高质量精度(&lt；2ppm)以及通过电子捕获解离(ECD)、红外多光子解离(IRMPD)和碰撞诱导解离(CID)进行的肽序列测定。蛋白质组学服务包括蛋白质ID；翻译后修饰的特征(例如，磷脂)；以及完整蛋白质的分析。该资源还为广泛的其他研究应用提供质谱学服务，如天然产品、药物和药物-聚合物结合物、核酸、DNA扩增产物(例如，聚合酶链式反应)、大生物分子和聚合物、多肽和合成小分子的结构分析。自2003年以来，查德·纳尔逊博士在两名工作人员的协助下，领导了MSP共享资源项目。MSP共享资源的仪器和工作人员位于中央位置。MSP共享资源服务于所有基于HCI实验室的科学项目(细胞生长和分化的核控制；细胞反应和调节；成像、诊断和治疗；以及胃肠道癌症)，描述在癌症机制中发挥作用的分子，特别是蛋白质和翻译后修饰。此外，MSP共享资源对小分子化合物的表征，包括天然产物和药物释放聚合物，对HCI的发育治疗活动有很大贡献。MSP共享资源是一个机构管理的设施，受到大学和HCI领导层的监督。有一个学院咨询委员会，并通过年度调查对资源进行用户满意度审查。该设施是按服务收费(按存储容量使用计费)运营的。2008年，接受同行评审资助的癌症中心成员和癌症中心共享资源的MSP共享资源的总体使用率为31%。请总协调小组提供资金，以支付拟议资源预算的14%(44,857美元)。MSP共享资源有足够的容量供癌症中心成员额外使用。