In Vivo Imaging of Heart Disease and Host-Pathogen Processes

心脏病和宿主病原体过程的体内成像

• 批准号: 7796321
• 负责人: Roberta A. Gottlieb
• 金额: $ 39.55万
• 依托单位: SAN DIEGO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-18 至 2012-03-17
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7796321
• 关键词: Anesthesia procedures; Animal Model; Animals; Arts; Award; Basic Science; Biological Process; Biomedical Research; Cell physiology; Cells; Clinical Trials Design; Collaborations; Detection; Development; Experimental Designs; Faculty; Fluorescence Microscopy; Funding; Future; Goals; Heart; Heart Diseases; Image; Immune response; Individual; Infarction; Infection; Inflammation; Lead; Life; Measures; Modality; Monitor; Mus; Patients; Process; Productivity; Proteins; Recruitment Activity; Reporter; Research; Research Personnel; Research Project Grants; Resolution; Signal Transduction; Stem cells; System; Time; Transgenic Animals; Transgenic Mice; Translations; United States National Institutes of Health; Virus Diseases; clinical application; cost; human disease; imaging modality; in vivo; instrument; member; mouse model; novel; pathogen; public health relevance

DESCRIPTION (provided by applicant): The Caliper Spectrum IVIS (in ivo imaging system) will be used to understand critical cellular processes in live mice using fluorescent or bioluminescent cells, proteins, or probes. Investigators are focused on heart disease or host response to infection, and have developed a range of transgenic animals or constructs that will enable them to utilize the imaging system for detection of cellular processes. This instrument images whole mice (under anesthesia) and can detect fluorescent or bioluminescent signals within the intact animal with a resolution of approximately 1 mm. This will allow investigators to detect nests of bioluminescent stem cells within the heart, to measure infarct size, or to monitor the progression of bacterial or viral infections over time. This instrument obviates the need to sacrifice animals at individual time points and will expand the modalities available for experimental design. Six NIH-funded investigators (representing 8 R01 awards and 3 investigators on a P01 award) will benefit from the instrument, and it is anticipated that additional faculty members will use the instrument in the future. The IVIS Spectrum will provide an important imaging modality that will extend the research capabilities of the investigators. Given the expertise of the group in fluorescence microscopy and in the development of transgenic mouse models for monitoring biological processes, it is expected that the IVIS will be rapidly incorporated into existing research projects. It will help to fulfill the important NIH goal of reducing the number of animals used in research, and this reduction in animal costs will extend the research dollars and increase productivity. The availability of the IVIS will facilitate new collaborations and will allow investigators to think about asking new questions, or taking novel experimental approaches. The availability of this state-of-the-art instrument will help SDSU recruit new biomedical research- oriented faculty as well as to retain its best investigators. This instrument will be extremely important to investigators wishing to apply their findings to patients, as the animal studies enhanced by the IVIS will lead the way to optimal clinical trial design. PUBLIC HEALTH RELEVANCE: The ability to conduct real-time, sequential in vivo imaging of small animals is an important extension of animal models developed to study human diseases, and a prerequisite to translation of basic science discoveries to clinical applications. This proposal is to request an in vivo imaging system (Caliper Spectrum) to study mice using fluorescent or bioluminescent reporters. The investigators contributing to this proposal will use the IVIS to study heart disease, inflammation, and infections.

描述（由申请人提供）：Caliper Spectrum IVIS（体内成像系统）将用于使用荧光或生物发光细胞、蛋白质或探针来了解活体小鼠的关键细胞过程。研究人员专注于心脏病或宿主对感染的反应，并开发了一系列转基因动物或构建体，使他们能够利用成像系统来检测细胞过程。该仪器对整只小鼠进行成像（在麻醉下），并且可以以大约 1 毫米的分辨率检测完整动物内的荧光或生物发光信号。这将使研究人员能够检测心脏内生物发光干细胞的巢，测量梗塞面积，或监测细菌或病毒感染随时间的进展。该仪器消除了在各个时间点处死动物的需要，并将扩展可用于实验设计的模式。六名 NIH 资助的研究人员（代表 8 名 R01 奖项的研究人员和 3 名 P01 奖项的研究人员）将从该仪器中受益，预计未来将有更多的教职员工使用该仪器。 IVIS Spectrum 将提供一种重要的成像方式，扩展研究人员的研究能力。鉴于该小组在荧光显微镜和开发用于监测生物过程的转基因小鼠模型方面的专业知识，预计 IVIS 将迅速纳入现有的研究项目。它将有助于实现美国国立卫生研究院减少研究中使用的动物数量的重要目标，而动物成本的降低将增加研究资金并提高生产力。 IVIS 的可用性将促进新的合作，并使研究人员能够考虑提出新问题或采取新颖的实验方法。这种最先进仪器的使用将有助于圣地亚哥州立大学招募新的生物医学研究型教师并留住最优秀的研究人员。该仪器对于希望将其研究结果应用于患者的研究人员来说极其重要，因为 IVIS 增强的动物研究将引导最佳临床试验设计。 公共卫生相关性：对小动物进行实时、连续的体内成像的能力是为研究人类疾病而开发的动物模型的重要延伸，也是将基础科学发现转化为临床应用的先决条件。该提案要求使用体内成像系统（Caliper Spectrum）来使用荧光或生物发光报告基因来研究小鼠。参与该提案的研究人员将使用 IVIS 研究心脏病、炎症和感染。