Regulation of the Dynamic Proteome after Ischemic Injury

摘要 这项工作的前提是基于积累的证据，即线粒体重塑发生在 应激性心脏缺血损伤后，不仅发生在高危区域，而且还发生在存活的区域 现在必须改变其工作能力的心肌。我们先前发现缺血和再灌流 导致有丝分裂和生物发生在RNA翻译水平上受到调控。这个应用程序将探索这一点 通过检测心脏中活性多聚体的动态蛋白质组成进一步发现 缺血应激。目标1将检验这样一种假设，即调节分子与多聚体在 在此过程中，选择线粒体靶向的mRNAs进行主动翻译。它将进一步 确定miRNAs，或者更确切地说，某些miRNAs的丢失是否也支配着这一过程。本项目的成果 将开发一份新合成的蛋白质在缺血恢复过程中的详细画像，使用 使用与质谱学兼容的Met类似物(叠氮高丙氨酸，AHA)进行代谢标记以定义新的 蛋白质蛋白质组。目标2中的工作将定义线粒体功能改变和 改变蛋白质乙酰化和转录输出的代谢体的变化。这一目标的工作将雇用 干扰糖酵解和氧化磷酸化的各种干预措施，表征代谢组，以及 结果与蛋白质乙酰化和转录组的特定变化相关，包括mRNA和 MiRNA。还将进行实验，以探索扰动，包括高脂肪饮食和 线粒体单倍群[使用胞质细胞和线粒体核交换(MNX)小鼠品系]。这个 多组学分析将需要开发新的生物信息学工具来识别关系和控制 节点通过代谢物水平的变化来调节。我们已经确定了一条共同的道路 通过吡格列酮和GLP1ra增加NADH/NAD比率，灭活sirtuins，导致蛋白质增加 乙酰化，降低miR-33的表达和活性。这些研究将揭示 线粒体生物发生的翻译控制，这可能导致开发新的治疗工具 在缺血/再灌注损伤的背景下调节这一过程。第二和第三个目标将产生一个新的 了解新陈代谢和线粒体输出对转录的作用，并有望 确定可能控制心脏重构的转录调控中特别重要的代谢物。 这一项目的重点是脑梗塞后重塑的机制，并将产生一个新的理解 存活心肌中线粒体功能、代谢输出和转录调控之间的联系 脑梗塞后。

ABSTRACT The premise of this work is based on the accumulated evidence that mitochondrial remodeling takes place in the stressed heart after ischemic injury, and occurs not only in the area at risk, but also in the remaining viable myocardium that must now alter its work capacity. We previously discovered that ischemia and reperfusion resulted in mitophagy and biogenesis regulated at the level of RNA translation. This application will explore this finding further by examining the dynamic protein composition of the active polysomes in hearts subjected to ischemic stress. Aim 1 will test the hypothesis that regulatory molecules associate with polysomes during ischemic stress and, in doing so, select mitochondria-targeted mRNAs for active translation. It will further determine if miRNAs, or rather the loss of certain miRNAs, also govern this process. Results from this project will develop a detailed portrait of the newly-synthesized proteins in the setting of recovery from ischemia, using metabolic labeling with a mass spectrometry-compatible Met analog (azidohomoalanine, AHA) to define the new protein proteome. Work in Aim 2 will define the relationship between alterations in mitochondrial function and changes in the metabolome that alter protein acetylation and transcriptional output. Work in this aim will employ various interventions to perturb glycolysis and oxidative phosphorylation, characterize the metabolome, and correlate results with specific alterations in protein acetylation and the transcriptome, including both mRNA and miRNA. Experiments will also be performed to explore perturbations, including a high-fat diet and variations in mitochondrial haplogroup [using cybrid cells and the mitochondrial nuclear exchange (MNX) mouse lines]. The multi-omics analysis will require the development of novel bioinformatics tools to identify relationships and control nodes mediated through changes in metabolite levels. We have already identified a common pathway shared by pioglitazone and GLP1Ra which increase the NADH/NAD+ ratio, inactivate sirtuins, result in increased protein acetylation, and lower the expression and activity of miR-33. These studies will reveal the mechanisms governing translational control of mitochondrial biogenesis, which may lead to the development of new therapeutic tools for regulating this process in the setting of ischemia/reperfusion injury. The second and third aims will yield a new understanding of the role that metabolism and mitochondrial output has on transcription, and is expected to identify metabolites of particular importance in transcriptional regulation that may govern cardiac remodeling. This project is focused on mechanisms of post-infarction remodeling and will yield a new understanding of the link between mitochondrial function, metabolic output, and transcriptional regulation in the surviving myocardium after infarction.