Illumina Genome Analyzer IIx for UMass Deep Sequencing Core

用于麻省大学深度测序核心的 Illumina 基因组分析仪 IIx

• 批准号: 7794425
• 负责人: SCOT A WOLFE
• 金额: $ 48.93万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-18 至 2011-08-17
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794425
• 关键词: Architecture; Area; Cardiovascular Diseases; Core Facility; DNA Binding; DNA Binding Domain; DNA Sequencing Facility; Diabetes Mellitus; Elements; Family; Funding; Generations; Genome; Genomics; Laboratories; Malignant Neoplasms; Maps; Methods; Population; Request for Applications; Research Personnel; Schizophrenia; Services; Specificity; United States National Institutes of Health; cell type; functional genomics; human disease; improved; instrument; medical schools; meetings

DESCRIPTION (provided by applicant): This application requests funding for an Illumina (Solexa) Genome Analyzer IIx massively parallel sequencer to enhance the capacity and capabilities of the Deep Sequencing Core Laboratory at UMass Medical School. Using a first generation Solexa instrument available in the core facility a number of laboratories have developed methods for mapping functional genomic elements and genomic architecture, identifying populations of small ncRNAs present in various species and cell types, and determining DNA-binding specificities for entire families of DNA-binding domains. The demand for this sequencing service is outstripping the present capacity of our core facility; the number of new users is growing rapidly with NIH-funded projects in areas of human disease such as ALS, Schizophrenia, Diabetes, Cancer and Cardiovascular disease. Addition of another deep sequencing instrument with improved capacity and capabilities is critical to meet the needs of these NIH-funded investigators. Twenty-four NIH funded projects that will directly benefit from this new capacity are described within our application.

描述（由申请人提供）： 该申请要求为Illumina（Solexa）基因组分析仪iix iix提供资金，以增强UMASS医学院深序核心实验室的能力和能力。使用核心设施中可用的第一代Solexa仪器，许多实验室开发了绘制功能性基因组元素和基因组结构的方法，鉴定了各种物种和细胞类型中存在的小NCRNA种群，并确定DNA结合的特异性，用于DNA结合域的整个家族。对这种测序服务的需求超过了我们核心设施的当前能力； NIH资助的项目在人类疾病（例如ALS，精神分裂症，糖尿病，癌症和心血管疾病）中，新用户的数量正在迅速增长。增加另一种具有提高能力和能力的深层测序工具对于满足这些NIH资助的研究人员的需求至关重要。我们的应用中描述了24个NIH资助的项目，这些项目将直接从这种新的能力中受益。