Typhoon Trio Phosphor Imaging System

描述（由申请人提供）：本提案旨在获得资金购买台风三重成像仪。这样的成像器允许从多种形式的分子、生物和生物化学实验中捕获、图像构建、定量分析和产生出版物图像，包括但不限于Southern和北方印迹、DNA结合测定、DNA足迹、蛋白质凝胶和免疫印迹。Typhoon Trio能够在放射自显影、荧光和荧光介质上执行这些操作，从而可以快速有效地对生成的数据进行定量。Typhoon Trio允许在一次实验中分析多个参数。从Southern和北方印迹、EMSA、DNA足迹、多色免疫印迹、二维分离到薄层色谱等技术将使用Typhoon Trio进行成像和定量。在这个应用程序中，用户组的18个生产实验室在五个部门已经组装。该小组的研究活动范围广泛，包括调查传染病免疫反应的调节、自身免疫、细菌和病毒发病机制、发育和生育、基因组完整性和基因调节、人类遗传性疾病和代谢紊乱、智力迟钝、肌肉再生、疫苗开发和视力障碍。从广义上讲，这些研究领域的目的是了解和寻找癌症，传染病，治疗儿童和成人遗传性疾病和肌肉萎缩的治疗方法。仪器的高吞吐量、较短的设置和培训时间以及台风三重奏产生的数据质量将提高这一多样化调查小组的科学生产力。

DESCRIPTION (provided by applicant): This proposal seeks to obtain funds to purchase a Typhoon Trio Imager. Such imagers allow the capture, image construction, quantitative analyses, and production of publication images from numerous forms of molecular, biological, and biochemical experiments, including but not limited to Southern and northern blots, DNA binding assays, DNA footprinting, protein gels, and immunoblots. The Typhoon Trio is able to perform these operations on autoradiographic, chemiluminescent, and fluorescent medias, allowing rapid and efficient quantitation of the data generated. The Typhoon Trio allows multiple parameters to be analyzed in a single experiment. Technologies ranging from Southern and northern blotting, EMSA, DNA footprinting, multi-color immunoblotting, two-dimensional separations, to thin layer chromatography will be imaged and quantitated using the Typhoon Trio. In this application, a user group of 18 productive laboratories in five departments has been assembled. The research activities of this group is broad and includes investigation into the regulation of the immune response in infectious disease, autoimmunity, mechanisms of bacterial and viral pathogenesis, development and fertility, genome integrity and gene regulation, human inherited diseases and metabolic disorders, mental retardation, muscle regeneration, vaccine development, and vision disorders. Broadly, these areas of investigation are aimed at understanding and finding cures for cancer, infectious disease, treating childhood and adult inherited diseases, and muscle wasting. The high through put of the instrumentation, the short set up and training time, and the quality of the data produced by the Typhoon Trio will increase the scientific productivity of this diverse group of investigative teams.