GE TYPHOON 9410 VARIABLE MODE IMAGER

GE Typhoon 9410 可变模式成像仪

• 批准号: 7793702
• 负责人: JOHN Rutledge BERMINGHAM
• 金额: $ 14.9万
• 依托单位: MC LAUGHLIN RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-18 至 2011-02-17
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793702
• 关键词: Acrylamides; Arts; Biomedical Research; Computer software; Computers; Detection; Disease; Equipment; Fluorescence; Gel; Genotype; Hour; Image; Label; Membrane; Montana; Myelin; Neurodegenerative Disorders; Nucleic Acids; Parkinson Disease; Proteins; Radio; Radioactive; Radioisotopes; Radiolabeled; Reaction; Research; Research Institute; Research Technics; Resolution; Running; Services; System; Techniques; Tissues; Update; Western Blotting; falls; molecular dynamics; northern hybridization; public health relevance; radiotracer; research study

DESCRIPTION (provided by applicant): Imaging and quantifying labeled molecules is a critical component of many modern biomedical research techniques. For the last 11 years we have imaged the results of Southern and northern hybridizations, and RNAse protections on a Molecular Dynamics Phosophorimager SI. The results must be analyzed using software on an old Macintosh computer that runs obsolete System 8 software. Should the phosphorimager or computer fail, they cannot be serviced. After 11 years of use, the phosphorimaging screens have deteriorated past their useful lifetime. The McLaughlin Research Institute is an independent non-profit research institute in Great Falls, Montana, three hours' drive away from other facilities with phosphor imaging capability. Although Southern and northern hybridization experiments continue to rely on radio isotopic labeling, fluorescent labeling techniques continue to advance, with new applications in imaging nucleic acids and proteins. We need to replace our phosphor imager with one that is capable of imaging and quantifying both radioactive and fluorescently labeled molecules. We are requesting the General Electric Typhoon 9410 Variable Mode Imager. In addition to functioning as a traditional phosphor imager, the Typhoon 9410 can detect red, green and blue fluorescence, permitting multiple nucleic acids and proteins to be detected simultaneously. This fluorescent capability will permit us perform multiplex PCR reactions for genotyping, as well as detection of proteins transferred to membranes from acrylamide gels (western blots) or from tissue sections (histoblots). The Typhoon 9410 has 10 micron resolution, a particularly useful feature for histoblots. The updated phosphor imaging capabilities that are provided by this equipment will permit us to more efficiently and reliably perform experiments using radio labeled nucleic acids, but will expand our capability to perform state-of-the-art techniques using labeled proteins as well. Public Health Relevance: To image and quantify radiolabelled molecules that are essential for our research, we have relied on a Molecular Dynamics Phosophorimager SI, whose capabilities have deteriorated considerably over the last 11 years. Although radioisotope labeling remains a useful research technique, fluorescent labeling techniques continue to advance. We need to replace our Phosphor imager with one that is capable of imaging and quantifying both radioactive and fluorescently Labeled molecules to advance our research, which is relevant to neurodegenerative diseases, Parkinson's disease and myelin diseases.

描述（由申请人提供）：标记分子的成像和定量是许多现代生物医学研究技术的关键组成部分。在过去的11年里，我们已经成像的结果，南方和北方杂交，和RNA酶的保护上的分子动力学Phosophorimager SI。结果必须使用运行过时System 8软件的旧Macintosh计算机上的软件进行分析。如果磷光成像仪或计算机出现故障，则无法对其进行维修。在使用11年后，磷光成像屏幕已经超过其使用寿命。麦克劳克林研究所是一个独立的非营利研究机构，位于蒙大拿州的大福尔斯，距离其他具有荧光成像能力的设施有三个小时的车程。尽管Southern和北方杂交实验继续依赖于放射性同位素标记，但荧光标记技术继续发展，在核酸和蛋白质成像方面有了新的应用。我们需要用一个能够成像和量化放射性和荧光标记分子的荧光成像仪来取代我们的荧光成像仪。通用电气Typhoon 9410可变模式成像仪除了作为传统的荧光成像仪，Typhoon 9410还可以检测红色、绿色和蓝色荧光，允许同时检测多种核酸和蛋白质。这种荧光能力将允许我们进行多重PCR反应进行基因分型，以及检测从丙烯酰胺凝胶（蛋白质印迹）或从组织切片（组织印迹）转移到膜上的蛋白质。Typhoon 9410具有10微米的分辨率，这是一个对组织印迹特别有用的功能。该设备提供的更新的磷光体成像能力将使我们能够更有效和可靠地使用放射性标记的核酸进行实验，但也将扩大我们使用标记蛋白质进行最先进技术的能力。 公共卫生相关性：为了对我们研究中必不可少的放射性标记分子进行成像和定量，我们依赖于分子动力学Phosophorimager SI，其能力在过去11年中大大恶化。虽然放射性同位素标记仍然是一种有用的研究技术，荧光标记技术继续发展。我们需要更换我们的 荧光成像仪，能够对放射性和荧光进行成像和定量 标记分子来推进我们的研究，这与神经退行性疾病有关， 帕金森病和髓鞘疾病。