In vivo non-invasive 3D quantitative IVIS Spectrum molecular imaging system

体内非侵入3D定量IVIS Spectrum分子成像系统

• 批准号: 7791805
• 负责人: Evan T Keller
• 金额: $ 35.99万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-01 至 2011-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7791805
• 关键词: Advisory Committees; Animals; Award; Biology of Aging; Bioluminescence; Cancer Center; Cells; Commit; Dental Schools; Disease; Ensure; Equipment; Evaluation; Fluorescence; Funding; Goals; Growth; Health; Image; Imagery; In Vitro; K22 Award; Label; Life; Location; Maintenance; Malignant Neoplasms; Medical; Michigan; Monitor; Mus; Neoplasm Metastasis; Reporter; Reporting; Research; Research Personnel; Research Project Grants; Scanning; Source; System; TNFRSF5 gene; Technology; Time; Tissues; Training; United States National Institutes of Health; Universities; Walking; age related; angiogenesis; animal facility; base; bone; cost; design; efficacy testing; experience; improved; in vivo; member; molecular imaging; public health relevance; research study; therapeutic angiogenesis; transmission process

DESCRIPTION (provided by applicant): The objective of this proposal is to acquire a non-invasive 3D quantitative molecular imaging system that allows for in vivo, ex vivo and in vitro imaging. The system, called the IVIS Spectrum from Caliper LifeSciences, is capable of transmission and reflectance fluorescence and bioluminescence imaging. It has 10 narrow band excitation filters and 18 narrow band emission filters which allow for scanning over a large wavelength region and evaluation of multiple reporters. The equipment allows for visualization of reporters located within deep tissue sources in vivo in anesthetized animals. In concrete terms, cells can be labeled with specific luminescent reports and injected into mice and their eventual localization and growth identified in mice. Specific examples include metastasis studies or quantification of angiogenesis. This application supports 12 investigators and a research portfolio of 13 R01s, 4 projects in one P01, 3 projects in two P50 SPORES, 2 R21s and 1 each of a K22 award, NIH Director's Award, and project in a U award for a total of 25 NIH-funded projects. The group consists of members of multiple Departments and the Medical and Dental Schools. Currently, there is one IVIS Spectrum system on campus, but at a 15 minute walk from the Cancer Center animal facility (a particular challenge for transporting animals, especially in winter) and often has lengthy wait periods (several weeks) to obtain access resulting in great challenges to planning in vivo experiments that can be very hard to time. The principal rationale for purchase of this system is to gain access to this technology so that in vivo experiments can be better planned due to less congested access to machine time and to have the equipment in a location readily accessible to the Cancer Center Building animal facility for a large number of investigators. The projects already planned for will utilize 87% of the annual user time indicating the high demand for this new equipment. Research projects are directly related to NIH goals of improving health and include studies on cancer metastasis, cancer therapeutics, angiogenesis, bone biology and age-related disease. The equipment will be supported by an experienced technical staff and an internal advisory committee. A system to attract and train new users is in place so that other NIH-funded investigators will benefit from the equipment. Significant institutional support for both maintenance and technical costs has been committed for the initial four years to ensure long-term viability of the system (Total of $151,600 over 4 years). A user-based recharge system will be put into place after this time period to continue its long-term use. Additionally, there is institutional commitment of space for the equipment. In summary access to this cutting edge imaging system will greatly accelerate the pace of research at University of Michigan for a large number of NIH-funded investigators. Public Health Relevance: We are requesting an imaging system that allows us to detect and quantify specific cells growing within living animals over long time periods. This will allow us to identify mechanisms of disease or monitor efficacy of test therapies. Ultimately, it will enable us to design or optimize treatments for disease.

描述（由申请人提供）：本提案的目的是获得一种非侵入性 3D 定量分子成像系统，可进行体内、离体和体外成像。该系统名为 Caliper LifeSciences 的 IVIS Spectrum，能够进行透射和反射荧光以及生物发光成像。它具有 10 个窄带激发滤光片和 18 个窄带发射滤光片，可在较大的波长区域内进行扫描并评估多个报告器。该设备允许对位于麻醉动物体内深层组织源内的报告基因进行可视化。具体而言，细胞可以用特定的发光报告标记并注射到小鼠体内，并确定它们在小鼠体内的最终定位和生长。具体例子包括转移研究或血管生成的量化。该应用程序支持 12 名研究人员和 13 个 R01 的研究组合、一个 P01 中的 4 个项目、两个 P50 SPORES 中的 3 个项目、2 个 R21 以及 K22 奖、NIH 主任奖和 U 奖各 1 个项目，总共 25 个 NIH 资助的项目。该小组由多个部门以及医学院和牙科学校的成员组成。目前，校园内有一个 IVIS Spectrum 系统，但距离癌症中心动物设施有 15 分钟步行路程（对于运输动物来说是一个特殊的挑战，尤其是在冬天），并且通常需要漫长的等待时间（几周）才能获得访问权限，这给规划体内实验带来了巨大的挑战，而且很难确定时间。购买该系统的主要理由是获得该技术的使用权，以便可以更好地规划体内实验，因为机器时间不会那么拥挤，并且可以将设备放置在癌症中心为大量研究人员建造动物设施的地方。已规划的项目将利用 87% 的年度用户时间，表明对这种新设备的高需求。研究项目与 NIH 改善健康的目标直接相关，包括癌症转移、癌症治疗、血管生成、骨生物学和年龄相关疾病的研究。该设备将由经验丰富的技术人员和内部咨询委员会提供支持。吸引和培训新用户的系统已经到位，以便其他 NIH 资助的研究人员能够从该设备中受益。最初四年承诺对维护和技术成本提供大量机构支持，以确保系统的长期可行性（四年总计 151,600 美元）。在此期限后，将建立基于用户的充值系统，以继续长期使用。此外，还有机构对设备空间的承诺。总之，使用这一尖端成像系统将大大加快密歇根大学大量 NIH 资助的研究人员的研究步伐。 公共卫生相关性：我们需要一种成像系统，使我们能够检测和量化活体动物体内长期生长的特定细胞。这将使我们能够确定疾病机制或监测测试疗法的功效。最终，它将使我们能够设计或优化疾病的治疗方法。