Shared LSRII Cytometer Equipment Application - UC Davis Stem Cell Program

共享 LSRII 细胞仪设备应用 - 加州大学戴维斯分校干细胞计划

• 批准号: 7792823
• 负责人: Jan A. Nolta
• 金额: $ 49.98万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-26 至 2011-01-25
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7792823
• 关键词: Area; Arts; Biology; Cancer Center; Cell Separation; Cells; Collaborations; Color; Core Facility; Custom; Development; Ensure; Equipment; Flow Cytometry; Fluorochrome; Funding; Future; Grant; Institutes; Lasers; Location; Medical center; Mind; NCI-Designated Cancer Center; Optics; Publications; Research; Research Personnel; Stem cells; Technology; United States National Institutes of Health; base; design; instrument; instrumentation; programs; stem cell biology; success; tool

DESCRIPTION (provided by applicant): Multicolor flow cytometry has become a powerful tool in many areas of research, and UC Davis has been at the forefront of this technology since 2000. With the development of the first custom designed 12-color, 14 parameter MoFlo Cell Sorter, UCD investigators have been developing multi-color panels for research. This research has generated over 250 publications and more than 200 NIH grants, including 57 NIH grants this year alone. The UCD Opticore was formed in 1995 on the Davis Campus, expanded to the NCI designated Cancer Center in Sacramento in 2002, and expanded again in 2006 to a second Sacramento location in collaboration with the new Stem Cell Program. The Sacramento facilities are accessible to a wide range of programs, including the UCD Medical Center, the MIND Institute, the Stem Cell Program, and the Cancer Center. Currently, 26 NIH grants based at UCD-Sacramento are dependent on access to a multi- parameter flow cytometer. However, these projects are limited to the use of a cell sorter, which was purchased for the Stem Cell Biology program. This sorter requires a dedicated operator, is already at greater than 50% user capacity and continuing to grow only a year after the facility opening. The acquisition of a self-operated, multi-parameter analytical flow cytometer will be critical to the success of the 26 NIH-funded research programs, many of which do not require cell sorting on a routine basis. The LSR-II cytometer is the only benchtop analysis instrument with five laser capability and the capacity to acquire up to 18 parameters, including 16 different fluorochromes, with additional capacity for future expansion. This state-of-the-art instrument will allow us to readily transfer the analytical panels already developed, plus add the additional applications and fluorochromes required for specific research programs as described in this application. Placing the instrument in the Optical Biology Core facility will ensure that the instrument is carefully maintained, and investigators will have maximum access to flow cytometry instrumentation and expertise.

描述（由申请人提供）：多色流式细胞术已成为许多研究领域的强大工具，加州大学戴维斯分校自2000年以来一直处于这项技术的前沿。随着第一台定制设计的12色，14参数MoFlo细胞分选仪的开发，UCD的研究人员已经开发出多色面板用于研究。这项研究已经发表了250多篇论文，获得了200多项NIH资助，其中仅今年就获得了57项NIH资助。UCD Opticore于1995年在戴维斯校区成立，2002年扩展到位于萨克拉门托的NCI指定癌症中心，并于2006年再次扩展到萨克拉门托的第二个地点，与新的干细胞项目合作。萨克拉门托的设施可供广泛的项目使用，包括UCD医疗中心、MIND研究所、干细胞项目和癌症中心。目前，加州大学萨克拉门托分校的26项NIH拨款依赖于多参数流式细胞仪。然而，这些项目仅限于使用细胞分选器，这是为干细胞生物学计划购买的。这种分拣机需要一个专门的操作员，已经有超过50%的用户容量，并且在设施开放后仅一年就继续增长。获得一台自助式、多参数分析流式细胞仪将对26个nih资助的研究项目的成功至关重要，其中许多项目不需要常规的细胞分选。LSR-II型细胞仪是唯一具有5个激光能力的台式分析仪器，能够获取多达18个参数，包括16种不同的荧光色，并具有未来扩展的额外容量。这种最先进的仪器将使我们能够轻松地转移已经开发的分析面板，并添加额外的应用程序和本应用程序中描述的特定研究计划所需的荧光染料。将仪器放置在光学生物学核心设施将确保仪器得到精心维护，研究人员将有最大限度地使用流式细胞仪仪器和专业知识。

项目成果

Efficient Generation of Induced Pluripotent Stem and Neural Progenitor Cells From Acutely Harvested Dura Mater Obtained During Ventriculoperitoneal Shunt Surgery.

• DOI: 10.1016/j.wneu.2015.05.076
• 发表时间: 2015-11
• 期刊: World neurosurgery
• 影响因子: 2
• 作者: Cary WA;Hori CN;Pham MT;Nacey CA;McGee JL;Hamou M;Berman RF;Bauer G;Nolta JA;Waldau B
• 通讯作者: Waldau B