800 MHz Triple Resonance Observe (TXO) Detection CryoProbe

描述（由申请人提供）：我们申请资金以获得800 MHz冷冻探针配件。我们已经获得了一个新的800兆赫仪器的资金，这将减轻长时间等待和高场仪器不足的情况。请求支持的研究人员小组（Gerhard瓦格纳、James Chou、Christopher沃尔什、Ellis Reinherz和Anders Naar）可用的工作频率为750 MHz及以上的高场仪器仅限于一台750 MHz频谱仪和一半的900 MHz系统。尽管这种有限的访问，这个小组是非常成功的，在确定结构和非常大的和具有挑战性的系统，如膜蛋白，翻译起始因子的复合物，非核糖体肽合成酶，转录激活因子/辅激活因子复合物，T细胞蛋白复合物和许多功能的研究。许多新项目正在筹备中，但由于无法获得高场仪器，进展缓慢，等待时间通常为两个月才能获得750或900 MHz频谱仪。为了缓解这一问题，我们已经从哈佛内部竞争和其他来源获得了购买非屏蔽800 MHz频谱仪的资金，并且我们已经获得资金来翻新哈佛/麻省理工学院磁共振中心的空间，以容纳新仪器。然而，可用的资金仅够购买带有常规室温探头的光谱仪，不足以购买低温探头。为了最佳地利用这种新仪器的能力，我们要求为冷冻探针配件提供资金，该冷冻探针配件具有一个冷冻平台和两个针对1H检测（TCI）进行优化的三重共振冷冻探针，这将是3D和4D分散NOESY实验的工作马，以及13 C/15 N检测优化探针，这将用于新的实验，有望推动研究超大型系统的极限。

DESCRIPTION (provided by applicant): We request funding to acquire a 800 MHz cryoprobe accessory. We have secured funding for a new 800 MHz instrument that will alleviate the long waiting times for and insufficient access to high-field instrumentation. The high-field instruments operating at 750 MHz and above available to the group of investigators asking for this support (Gerhard Wagner, James Chou, Christopher Walsh, Ellis Reinherz and Anders Naar) are limited to one 750 MHz spectrometer and half of a 900 MHz system. Despite this limited access, this group was very successful in determining structures and performing functional studies of very large and challenging systems, such as membrane proteins, complexes of translation initiation factors, non-ribosomal peptide synthetases, transcriptional activator/coactivator complexes, T-cell protein complexes and many more. Numerous new projects are in the pipeline but progress is slowed down due to the lack of access to high field instruments, and waiting times are often two months to get access to the 750 or 900 MHz spectrometers. To alleviate this, we have obtained funds to purchase a non-shielded 800 MHz spectrometer from a Harvard internal competition, and other sources, and we have secured funds to renovate space at the Harvard/MIT Center for Magnetic Resonance to house the new instrument. However, the funds available are only sufficient to purchase the spectrometer with a conventional room temperature probe and are not enough to buy a cryogenic probe. To optimally use the power of this new instrument we ask for funds for a cryoprobe accessory with one cryoplatform and two triple-resonance cryoprobes that are optimized for 1H detection (TCI), which will be the work horse for 3D ad 4D dispersed NOESY experiments, and a 13C/15N detection optimized probe, which will be used for novel experiments that promise to push the limit for studying very large system.