Mechanisms of Synaptic Specificity in C. elegans

线虫突触特异性的机制

• 批准号: 8018044
• 负责人: KANG SHEN
• 金额: $ 33.7万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-12-07 至 2015-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8018044
• 关键词: Alzheimer' s Disease; Axon; Axonal Transport; Brain; CDC2 Protein Kinase; Caenorhabditis elegans; Chemical Synapse; Communication; Cues; Cyclin-Dependent Kinases; Cyclins; Data; Dendrites; Development; Disease; Distal; Event; Feedback; Genetic; Genetic Screening; Grant; Guanosine Triphosphate Phosphohydrolases; Health; Kinesin; Light; Lobular Neoplasia; Location; Mediating; Microtubules; Modeling; Modification; Molecular; Molecular Motors; Motor; Neurodegenerative Disorders; Neurons; Pathogenesis; Pathway interactions; Pattern; Physiological; Presynaptic Terminals; Property; Proteins; Proteomics; Publishing; Recruitment Activity; Regulation; Research; Specific qualifier value; Specificity; Stereotyping; Structure; Synapses; Synaptic Vesicles; System; Testing; Transport Vesicles; Vesicle; base; extracellular; gene function; in vivo; insight; mutant; neuronal cell body; presynaptic; relating to nervous system; synaptogenesis; trafficking

DESCRIPTION (provided by applicant): Chemical synapses are specialized cellular junction structures that are essential for communication between neurons. During development, synapses form between specific neurons at defined subcellular compartments. Synaptic target selection, axonal transport and presynaptic assembly are integral steps of synapse formation that are poorly understood. Here, I propose to expand our research to understand two essential aspects of synapse formation: polarized axonal trafficking and how aggregation of active zone proteins is regulated. Synapses are usually formed on distal axon and dendrites, creating a challenging problem for effective exchange of intracellular material between cell bodies and synapses. Microtubules and MT associated motors mediate intracellular trafficking. It is generally believed that the direction of transport depends on two factors: the polarity of MTs and the type of motor involved. Based on our published and unpublished data, we have identified two cyclin-dependent kinase pathways that are essential for the trafficking of presynaptic components. In the absence of both pathways, the vast majority of synaptic vesicle proteins and active zone markers fail to localize to axon and instead are found in dendrites due to misregulation of kinesin motors. Another poorly understood question in synapse formation is how the pool size of synaptic vesicles is determined. Many synapses display stereotyped size of synaptic vesicle clusters, suggesting that molecular mechanisms regulate the assembly of synaptic vesicle precursors locally at the presynaptic terminals. When the appropriate number of vesicles is recruited, there might be a negative feedback system to shut down the assembly pathway. We reasoned that if this feedback mechanism is defective, one should expect to see mutant synapses with abnormal vesicle pool. Indeed in a forward genetic screen, we isolated a mutant in which the proximal synapses are abnormally large while the distal synapses contain little material. We have also identified an Arf like GTPase to be responsible for the regulation the location and size of presynaptic specializations. To gain mechanistic insights on the functions of these genes, I specifically plan to understand: 1) how the cyclin-dependent kinases pathways control axonal transport and synapse localization through the regulation of molecular motors, and 2) how an ARF like GTPase, ARL8 regulate the synaptic vesicle pool size and presynaptic assembly. Given that many neural disorders are associated with alterations in synaptic connectivity and that cyclin dependent kinases have been implicated in neurodegenerative diseases, it is hopeful that this project will help to understand brain development under both physiological and pathological conditions. PUBLIC HEALTH RELEVANCE: Synapses are critical for the communication between neurons. During synapse formation, proteins and vesicle are transported to presynaptic terminals in distal axons. The proposed study characterizes critical regulatory mechanisms for trafficking synaptic components to axons as well as locally synaptic assembly. This project will potentially shed light on the pathogenesis mechanisms of neurodegenerative diseases such as the Alzheimer's disease.

描述（由申请人提供）：化学突触是专门的细胞连接结构，对于神经元之间的通信至关重要。在发育过程中，突触在特定的亚细胞区室中的特定神经元之间形成。突触靶点选择、轴突运输和突触前组装是突触形成的重要步骤，但对这些步骤的了解还很少。在这里，我建议扩大我们的研究，以了解突触形成的两个重要方面：极化轴突运输和活动区蛋白的聚集是如何调节的。 突触通常形成在远端轴突和树突上，这为细胞体和突触之间的细胞内物质的有效交换带来了挑战性的问题。微管和MT相关马达介导细胞内运输。一般认为，转运的方向取决于两个因素：MT的极性和所涉及的电机类型。基于我们已发表和未发表的数据，我们已经确定了两个细胞周期蛋白依赖性激酶途径，是必不可少的运输突触前组件。在这两种途径的情况下，绝大多数突触囊泡蛋白和活性区标记物不能定位于轴突，而是由于驱动蛋白马达的误调节而在树突中发现。突触形成中另一个知之甚少的问题是突触囊泡的大小是如何决定的。许多突触显示定型大小的突触囊泡簇，表明分子机制调节突触囊泡前体在突触前末梢的组装。当合适数量的囊泡被募集时，可能会有一个负反馈系统来关闭组装途径。我们推断，如果这种反馈机制是有缺陷的，人们应该期待看到突变的突触与异常囊泡池。事实上，在正向遗传筛选中，我们分离出了一个突变体，其中近端突触异常大，而远端突触几乎不含物质。我们还确定了一个类似GTfos的Arf负责调节突触前特化的位置和大小。 为了获得对这些基因功能的机制性见解，我特别计划了解：1）细胞周期蛋白依赖性激酶途径如何通过调节分子马达来控制轴突运输和突触定位，以及2）ARF（如GT3，ARL8）如何调节突触囊泡池大小和突触前组装。鉴于许多神经疾病与突触连接的改变有关，并且细胞周期蛋白依赖性激酶与神经退行性疾病有关，因此希望该项目将有助于了解生理和病理条件下的大脑发育。 公共卫生相关性：突触对于神经元之间的通信至关重要。在突触形成过程中，蛋白质和小泡被转运到远端轴突的突触前终末。拟议的研究特征的关键监管机制，运输突触组件轴突以及本地突触组装。该项目将潜在地阐明神经退行性疾病如阿尔茨海默病的发病机制。