Patterning dendritic branches with environmental and neuronal surface molecules
用环境和神经元表面分子图案化树突分支
基本信息
- 批准号:8589246
- 负责人:
- 金额:$ 33.52万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2013
- 资助国家:美国
- 起止时间:2013-06-01 至 2018-05-31
- 项目状态:已结题
- 来源:
- 关键词:AdoptedAfferent NeuronsAllelesAutistic DisorderAxonBickers-Adams syndromeBindingBiochemicalCaenorhabditis elegansCell AdhesionCell Adhesion MoleculesCellsCharacteristicsComplexCuesDataDendritesDevelopmentDiseaseDrosophila genusEnvironmentGenesGeneticGenetic ScreeningGenetic TranscriptionGoalsGrantGrowthHumanImageInsectaIntegral Membrane ProteinInvestigationKnowledgeLengthLeucine-Rich RepeatLigandsLinkMedicalMembraneMental RetardationMolecularMorphogenesisMorphologyMutationNatural regenerationNervous system structureNeural Cell Adhesion Molecule L1Neurodegenerative DisordersNeuronsNociceptionPatternPhenotypePlayPrevention MeasuresProcessProteinsRNA SplicingResearchRett SyndromeRoleSamplingSchizophreniaShapesSignal PathwaySkinSpastic ParaplegiaStrokeStructureSurfaceSystemTestingTherapeuticTight JunctionsTimeVariantbasecombatcomputerized data processinggain of functioninformation gatheringinsightleucine-rich repeat proteinloss of functionmutantnervous system disorderneural circuitnoveloverexpressionprogramspublic health relevancereceptive fieldreceptorresearch studytherapy development
项目摘要
DESCRIPTION (provided by applicant): Dendritic arbors adopt diverse, branched morphologies of varying complexity that are characteristic for a given neuron type. The organization of the dendritic arbors is fundamental to the connectivity and function of a neuron. Although critical to the shape and connectivity of the nervous system, the mechanisms that regulate dendrite morphology are not well understood. In particular, our understandings of membrane molecules that promote and guide patterned dendritic branches are lacking. The overall goal of this grant is to identify novel molecule ligands and receptors that generate the complex and orderly dendrite branches of the nociceptive PVD neurons in C. elegans. Our hypothesis is that interactions between membrane molecules guide dendritic growth and branching in its environment. Following up on our recent discovery of a transmembrane leucine-rich repeat molecule DMA-1 that is essential for PVD dendritic morphogenesis, in specific aim 1, we will further understand the function of DMA-1 by further characterizing its loss-of-function phenotypes, testing its sufficiency and searching for its interaction partners. In specific aim 2, we will explore the function of cell adhesion L1CAM/SAX-7 in generating patterned dendritic branches. We will examine the loss- and gain-of-function phenotype of sax-7, determine its cellular requirement and examine its subcellular localization. We will also study the function of a
novel transmembrane protein W01F3.1, which we isolated from a forward genetic screen. In specific aim3, we will test if DMA-1, SAX-7 and W01F3.1 form a tripartite ligand-receptor complex that pattern PVD dendrite using genetic and biochemical means.
描述(由申请人提供):树枝状树木采用具有不同复杂性的多种分支形态,这些形态是给定神经元类型的特征。树突的组织对于神经元的连接和功能是至关重要的。虽然对神经系统的形状和连通性至关重要,但调节树突形态的机制还不清楚。特别是,我们缺乏对促进和引导模式化树突状分支的膜分子的理解。这项研究的总体目标是鉴定新的分子配体和受体,这些配体和受体产生C.优美的我们的假设是,膜分子之间的相互作用引导树突生长和分支的环境。继我们最近发现的跨膜富含亮氨酸的重复分子DMA-1是PVD树突状形态发生所必需的,在具体的目标1中,我们将通过进一步表征其功能丧失表型,测试其充分性并寻找其相互作用伙伴来进一步了解DMA-1的功能。在具体目标2中,我们将探索细胞粘附L1 CAM/SAX-7在产生图案化树突状分支中的功能。我们将研究sax-7的功能丧失和获得表型,确定其细胞需求并研究其亚细胞定位。我们还将研究
新的跨膜蛋白W01F3.1,我们从正向遗传筛选中分离。在特定的aim 3中,我们将测试DMA-1,SAX-7和W01F3.1是否形成三重配体-受体复合物,该复合物使用遗传和生物化学手段来形成PVD树突。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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KANG SHEN其他文献
KANG SHEN的其他文献
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{{ truncateString('KANG SHEN', 18)}}的其他基金
2017 Excitatory Synapses and Brain Function Gordon Research Conference and Gordon Research Seminar
2017兴奋性突触与脑功能戈登研究会议暨戈登研究研讨会
- 批准号:
9329815 - 财政年份:2017
- 资助金额:
$ 33.52万 - 项目类别:
Patterning dendritic branches with environmental and neuronal surface molecules
用环境和神经元表面分子图案化树突分支
- 批准号:
10311468 - 财政年份:2013
- 资助金额:
$ 33.52万 - 项目类别:
Patterning dendritic branches with environmental and neuronal surface molecules
用环境和神经元表面分子图案化树突分支
- 批准号:
9068348 - 财政年份:2013
- 资助金额:
$ 33.52万 - 项目类别:
Patterning dendritic branches with environmental and neuronal surface molecules
用环境和神经元表面分子图案化树突分支
- 批准号:
10579192 - 财政年份:2013
- 资助金额:
$ 33.52万 - 项目类别:
Patterning dendritic branches with environmental and neuronal surface molecules
用环境和神经元表面分子图案化树突分支
- 批准号:
8659526 - 财政年份:2013
- 资助金额:
$ 33.52万 - 项目类别:
2010 Molecular and Cellular Neurobiology Gordon Research Conference
2010年分子和细胞神经生物学戈登研究会议
- 批准号:
7912022 - 财政年份:2010
- 资助金额:
$ 33.52万 - 项目类别:
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