Spiral dynamics in the cortex during seizure and sensory evoked activity

癫痫发作和感觉诱发活动期间皮质的螺旋动力学

• 批准号: 8018046
• 负责人: Jian-Young Wu
• 金额: $ 29.62万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-15 至 2014-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8018046
• 关键词: Area; Bicuculline; Brain; Carbachol; Cardiac; Data; Development; Dyes; Electrophysiology (science); Epilepsy; Event; Goals; Heart; Image; Incidence; Lead; Life; Mission; Modeling; Morphologic artifacts; Motor Cortex; National Institute of Neurological Disorders and Stroke; Nature; Neocortex; Neurons; Noise; Pattern; Pattern Formation; Phase; Play; Population; Rattus; Reporting; Research Personnel; Resolution; Rodent; Role; Science; Seizures; Sensory; Sensory Process; Sensory Seizures; Signal Transduction; Sleep; Slice; Staining method; Stains; Stimulus; System; Testing; Therapeutic; Tissues; Vibrissae; Work; analytical method; barrel cortex; imaging modality; improved; in vivo; neocortical; prevent; programs; research study; response; sensory cortex; voltage

DESCRIPTION (provided by applicant): The long-term goal of this study is to understand the organization of cortical neuronal activity in sustained seizure events and in sensory processing. In this proposal, we will study a special form of population neuronal activity, namely, spiral waves. Spiral waves are a ubiquitous feature of excitable systems in nature, where they play a role in pattern formation and the organization of flow dynamics. Within biomedical science, spiral wave dynamics have been studied extensively in cardiac electrophysiology, and have greatly advanced our understanding of arrhythmogenic mechanisms. Surprisingly, however, only a few researchers have studied spiral dynamics in the brain. Following our recent discovery of spiral waves in rat neocortical slices, in this proposal, we will experimentally verify the existence of spiral waves in rodent neocortex in vivo during seizure-like events and during sensory evoked and spontaneous activity. Cortex in vivo has extensive long-range connections which are not present in brain slices. It is therefore necessary to experimentally examine the initiation and sustaining of spiral waves in intact cortex, given that strong long-range thalamocortical and corticocortical connections may disrupt the development of these spiral waves. Three Specific Aims are proposed to study wave-to-wave interactions in rat sensory and motor cortices. Aim 1 is devoted to improve voltage-sensitive dye imaging methods in order to identify phase singularities at the spiral center. Aim 2 is to examine the incidence rate of spirals during seizure-like activity in various cortical areas. Aim 3 is to investigate spiral dynamics during sensory-evoked activity and sleep-like waves. Studying spiral dynamics in the cortex will directly contribute to understanding of the initiation and sustaining of seizure activity. Spirals are known as a major contributor to arrhythmic activity in cardiac tissue, and extinguishing spirals in the heart has been a therapeutic strategy for preventing cardiac fibrillation. This project is highly relevant to the mission of the NINDS, and should contribute to the understanding of initiation and sustaining of epileptic activity in the cortex, which disturbs the life of about 1% of the US population.

描述（由申请人提供）：本研究的长期目标是了解持续癫痫事件和感觉处理中皮质神经元活动的组织。在此提案中，我们将研究一种特殊形式的人群神经元活动，即螺旋波。螺旋波是自然界中令人兴奋系统的无处不在特征，它们在模式形成和流动动态的组织中发挥作用。在生物医学科学中，螺旋波动力学在心脏电生理学中进行了广泛的研究，并大大提高了我们对心律不齐机制的理解。但是，令人惊讶的是，只有少数研究人员研究了大脑中的螺旋动力学。在我们最近在大鼠新皮层切片中发现了螺旋波之后，在此提案中，我们将在癫痫发作样事件和感官诱发和自发活动中实验验证体内啮齿动物新皮层中的螺旋波的存在。体内皮层具有广泛的远距离连接，而大脑切片中不存在。因此，鉴于强大的远程丘脑皮层和皮质皮层连接可能会破坏这些螺旋波的发展，因此有必要在完整皮层中实验检查螺旋波的起始和维持。提出了三个特定的目的，以研究大鼠感觉和运动皮层中的波浪之间的相互作用。 AIM 1致力于改善电压敏感的染料成像方法，以识别螺旋中心的相位奇异性。目标2是检查各种皮质区域癫痫发作活性期间螺旋的发病率。 AIM 3是在感官诱发的活动和睡眠状的波浪中研究螺旋动力学。研究皮质中的螺旋动力学将直接有助于理解癫痫发作活性的起始和维持。螺旋被称为心脏组织中心律失常的主要因素，心脏熄灭螺旋已成为预防心脏纤颤的治疗策略。该项目与Ninds的使命高度相关，应有助于理解皮质中癫痫活动的起始和维持癫痫活性，这破坏了约1％的美国人群的寿命。

项目成果

Evidence for glycinergic GluN1/GluN3 NMDA receptors in hippocampal metaplasticity.

海马化生中甘氨酸 GluN1/GluN3 NMDA 受体的证据。

• DOI: 10.1016/j.nlm.2015.10.005
• 发表时间: 2015
• 期刊: Neurobiology of learning and memory
• 影响因子: 2.7
• 作者: Rozeboom,AaronM;Queenan,BridgetN;Partridge,JohnG;Farnham,Christina;Wu,Jian-Young;Vicini,Stefano;Pak,DanielTS
• 通讯作者: Pak,DanielTS

In Vivo Voltage-Sensitive Dye Imaging of Mammalian Cortex Using "Blue" Dyes.

使用“蓝色”染料对哺乳动物皮层进行体内电压敏感染料成像。

• DOI: 10.1101/pdb.prot089359
• 发表时间: 2015
• 期刊: Cold Spring Harbor protocols
• 作者: Baker,Bradley;Gao,Xin;Wolff,BrianS;Jin,Lei;Cohen,LawrenceB;Bleau,ChunX;Wu,J-Y
• 通讯作者: Wu,J-Y

Interactions between two propagating waves in rat visual cortex.

• DOI: 10.1016/j.neuroscience.2012.04.062
• 发表时间: 2012-08-02
• 期刊: NEUROSCIENCE
• 影响因子: 3.3
• 作者: Gao, X.;Xu, W.;Wang, Z.;Takagaki, K.;Li, B.;Wu, J. -Y.
• 通讯作者: Wu, J. -Y.