Modulation of Receptor Number in Cultured Cells

培养细胞中受体数量的调节

基本信息

批准号：
8013819
负责人：
PATRICIA M. HINKLE
金额：
$ 35.13万
依托单位：
UNIVERSITY OF ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
起止时间：
1999-07-01 至 2013-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8013819
关键词：
Acute Address Animals Anterior Pituitary Gland Antibodies Arrestins Binding Biological Assay Bolus Infusion Cell membrane Cells Cultured Cells Dimerization Dissociation Endocytic Vesicle Endocytosis Endosomes Enzyme-Linked Immunosorbent Assay G-Protein-Coupled Receptors Goals Hormones Hypothalamic structure Knockout Mice Label Learning Measures Mole the mammal Mutate Organelles Output Pathway interactions Pattern Phosphorylation Phosphorylation Site Phosphotransferases Physiologic pulse Physiological Pituitary Gland Prolactin Protein Dephosphorylation Rattus Receptor Activation Receptor Signaling Recycling Role Signal Transduction Signal Transduction Pathway Site Staining method Stains Surface Techniques Temperature Testing Thyroid Gland Thyrotropin Thyrotropin-Releasing Hormone Thyrotropin-Releasing Hormone Receptors Tissues arrestin3 beta-arrestin desensitization immunocytochemistry in vivo inorganic phosphate mutant nutrition receptor receptor internalization receptor recycling research study response stoichiometry trafficking

项目摘要

Hypothalamic thyrotropin-releasing hormone (TRH) stimulates thyrotropin and prolactin release by the anterior pituitary gland. The the mechanisms of inactivation of the TRH receptor, a Ca2+-mobilizing G protein-coupled receptor, are not well understood. Desensitization results from receptor phosphorylation, beta-arrestin binding, and internalization. Resensitization requires receptor dephosphorylation and recycling. There is virtually no information about how the TRH receptor is actually used in vivo. We have recently developed a highly selective phospho-site specific antibody against the phosphorylated (i.e. recently activated) TRH receptor that allows us to address these issues. The phosphorylation sites recognized by the antibody are critical for receptor internalization and desensitization, and the antibody gives strong staining of pituitary tissue from TRH-injected rats. The goals of the proposal are to characterize TRH receptor phosphorylation, dephosphorylation, recycling and resensitization, and to establish how receptor activation occurs in vivo. The first aim uses newly available techniques to determine the stoichiometry of phosphorylation. The kinases involved will be identified, as will the phosphorylation sites on the receptor, which will then be mutated and the effects on signaling, traffickingand desensitization assessed. The next aim will test the hypothesis that dephosphorylation is critical for controlling the intracellular traffic of the internalized receptor. Rates of dephosphorylation of receptorson the plasma membrane and in endocytic vesicles will be measured, and the subcellular sites of dephosphosphorylation will be determined. The third aim tests the hypothesis that TRH receptor cycling and resensitization are controlled byphosphorylation- dependent Detaarrestinbinding. The pathway of TRH receptor recycling will be determined. The role of Detaarrestin in TRH receptor cycling and resensitizationwill be defined, as will the importance of receptor dimerization. Most experiments in these aims will be done using pituitary GH3 cells. The last aim capitalizes on the ability of the phospho-site specific antibody to identify recently activated TRH receptor. The antibody will be used to follow receptor phosphorylation following a bolus of TRH in rats and in TRH and TRH receptor knockout mice, and to test the hypothesis that hypothalamic TRH drive is responsible for changes in TSH output in vivo in response to changes in thyroid status, nutrition and temperature.

下丘脑甲状腺激素释放激素（TRH）刺激甲状腺激素和催乳素释放前垂体。 TRH受体失活的机理，一种Ca2+的g g 蛋白质偶联的受体尚不清楚。受体磷酸化，脱敏的结果， β-arrestin结合和内在化。复敏需要受体去磷酸化和回收利用。几乎没有关于如何实际在体内使用TRH受体的信息。我们最近有开发了针对磷酸化的高度选择性的磷酸位点特异性抗体（即最近激活的）TRH受体，使我们能够解决这些问题。由抗体对于受体内在化和脱敏至关重要，抗体对来自TRH注射大鼠的垂体组织。该提案的目标是表征TRH受体磷酸化，去磷酸化，回收和敏化，并建立受体激活如何发生在体内。第一个目的使用新近可用的技术来确定化学计量法磷酸化。涉及的激酶将被鉴定，受体上的磷酸化位点也将被鉴定然后将突变，并评估对信号传导，运输和脱敏的影响。下一个 AIM将检验以下假设：去磷酸化对于控制的细胞内流量至关重要内部化受体。受体去磷酸化的质膜和内吞作用的速率将测量囊泡，并确定去磷酸化的亚细胞位点。第三 AIM检验TRH受体循环和脱敏化的假设是由磷酸化控制的依赖的detaarrestinbinding。将确定TRH受体回收的途径。的作用在TRH受体循环和脱发化中，将定义Detaarrestin，受体的重要性也将二聚化。这些目标中的大多数实验都将使用垂体GH3细胞进行。最后的目标资本化关于磷酸位点特异性抗体鉴定最近活化的TRH受体的能力。抗体将在大鼠和TRH和TRH受体中的TRH推注后跟随受体磷酸化敲除小鼠，并检验下丘脑TRH驱动器的假设是TSH的变化响应甲状腺状态，营养和温度的变化而在体内输出。

项目成果

期刊论文数量（39）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

siRNA screen identifies the phosphatase acting on the G protein-coupled thyrotropin-releasing hormone receptor.

siRNA 筛选可鉴定作用于 G 蛋白偶联促甲状腺素释放激素受体的磷酸酶。

DOI：
10.1021/cb3004513
发表时间：
2013
期刊：
ACS chemical biology
影响因子：
4
作者：
Gehret,AustinU;Hinkle,PatriciaM
通讯作者：
Hinkle,PatriciaM

Regulation of endogenous melanocortin-4 receptor expression and signaling by glucocorticoids.

糖皮质激素对内源性黑皮质素 4 受体表达和信号传导的调节。

DOI：
10.1210/en.2006-0984
发表时间：
2006
期刊：
Endocrinology
影响因子：
4.8
作者：
Sebag,JulienA;Hinkle,PatriciaM
通讯作者：
Hinkle,PatriciaM

Activation of protein kinase C reduces L-type calcium channel activity of GH3 pituitary cells.

蛋白激酶 C 的激活会降低 GH3 垂体细胞的 L 型钙通道活性。

DOI：
10.1152/ajpcell.1992.262.5.c1211
发表时间：
1992
期刊：
The American journal of physiology
影响因子：
0
作者：
Haymes,AA;Kwan,YW;Arena,JP;Kass,RS;Hinkle,PM
通讯作者：
Hinkle,PM

A novel role for pigment genes in the stress response in rainbow trout (Oncorhynchus mykiss).

DOI：
10.1038/srep28969
发表时间：
2016-07-04
期刊：
Scientific reports
影响因子：
4.6
作者：
Khan UW;Øverli Ø;Hinkle PM;Pasha FA;Johansen IB;Berget I;Silva PI;Kittilsen S;Höglund E;Omholt SW;Våge DI
通讯作者：
Våge DI

Subcellular trafficking of the TRH receptor: effect of phosphorylation.

TRH 受体的亚细胞运输：磷酸化的影响。