Genomics, Proteomics and Bioinformatics

基因组学、蛋白质组学和生物信息学

• 批准号: 8076775
• 负责人: Terry L Thomas
• 金额: $ 16.56万
• 依托单位: TEXAS A&M UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8076775
• 关键词: Archives; Biochemical; Bioinformatics; Circadian Rhythms; Custom; DNA Microarray Chip; Data Analyses; Development; Disease; Gene Expression; Genome; Genomics; Goals; Ions; Laboratories; Lead; Mass Spectrum Analysis; Molecular; Pathology; Periodicity; Preparation; Protein Chemistry; Proteins; Proteomics; Research Personnel; Research Support; Sampling; System; Texas; Time; Work; Yeasts; base; comparative; data mining; multiple myeloma M Protein; new technology; protein structure; research study; tool; yeast two hybrid system

Core A- the Genomics, Proteomics and Bioinformatics Core The overall goal of Core A is to provide genomics, proteomics and bioinformatics support for P01 investigators. Core A will maintain whole genome and custom DNA microarray platforms to facilitate the gene expression experiments of P01 investigators. Core A will also provide quantitative real-time PCR (qPCR) support for P01 projects. Proteomics activities will be supported within Core A. This will include facilitating yeast two-hybrid screens and preparation of protein samples for Ion Trap Electrospray Mass Spectrometry (IE-MS) to be carried out in the Texas A&M Protein Chemistry Laboratory. Core A will provide robust bioinformatics platform(s) for P01 investigators. Local and distributed servers will be provided for data analysis, storage and archiving. Additional data mining tools will be maintained locally for further gene expression, protein structure, and comparative data analysis. Core A will acquire or develop new technologies to facilitate P01 investigator progress. As new genomics, proteomics and bioinformatics platforms emerge, they will be evaluated by Core A and implemented where appropriate. The research supported by the Genomics, Proteomics and Bioinformatics Core will lead to an understanding of the molecular and biochemical basis of circadian timing and of the systems-level consequences of circadian rhythmicity. This in turn will facilitate the development of new therapies for diseases and conditions related to circadian pathologies.

核心A-基因组学，蛋白质组学和生物信息学核心 核心A的总体目标是为P01提供基因组学，蛋白质组学和生物信息学支持 调查人员。核心A将保持整个基因组和自定义DNA微阵列平台，以促进 P01研究者的基因表达实验。核心A还将提供定量的实时PCR （QPCR）支持P01项目。蛋白质组学活动将在核心A中得到支持。这将包括 促进酵母双杂交筛网和离子陷阱电喷雾质量的蛋白质样品的制备 在德克萨斯州A＆M蛋白化学实验室进行的光谱法（IE-MS）。核心A将提供 P01研究人员的强大生物信息学平台。将为数据提供本地和分布式服务器 分析，存储和归档。其他数据挖掘工具将在本地维护以获取进一步的基因 表达，蛋白质结构和比较数据分析。核心A将获得或发展新的 促进P01研究者进度的技术。作为新的基因组学，蛋白质组学和生物信息学 平台出现，将通过核心A进行评估，并在适当的情况下实施。 基因组学，蛋白质组学和生物信息学核心支持的研究将导致理解 昼夜节律的分子和生化基础和系统级别的后果 昼夜节律。反过来，这将有助于开发针对疾病和状况的新疗法 与昼夜节律有关。