Copy of Live-cell wavefront metrology

活细胞波前测量的副本

• 批准号: ST/F001347/1
• 负责人: Ilan Davis
• 金额: $ 6.48万
• 依托单位: University of Oxford
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=ST%2FF001347%2F1
• 关键词: Copy; Live; cell; wavefront; metrology

The study of living organisms is increasingly based on studies of the dynamics of processes occurring within the living organism rather than, as in the past, the study of dead, stained, dehydrated and sectioned specimens. Wavefront sensing techniques used to measure the effects of atmospheric turbulence on high-precision imaging in astronomy provide a mechanism for the tracking of the fluorescent particles frequenlty used as tracers in live-cell imaging. This biology application has much in common with astronomy - the light levels available are low (stars are faint, bright fluorescent particles poisen the cells), the observation may be subject to time and space-varying distortions (due to the atmosphere in astronomy, due to the specimen itself in live-cell imaging), dependent on the application, different spectral (colour) regions or spectral bands may need to be used. This programme seeks to use wavefront sensing techniques developed for use in astronomy to the problems of imaging 3-dimensional and time-varying processes in biology. Many developmental studies of living specimens will benefit from a successful application of these techniques.

对活生物体的研究越来越多地基于对活生物体内部发生的过程的动力学研究，而不是像过去那样研究死亡、染色、脱水和切片的标本。波前传感技术用于测量大气湍流对天文学高精度成像的影响，为跟踪活细胞成像中经常用作示踪剂的荧光颗粒提供了一种机制。这种生物学应用与天文学有很多共同之处——可用的光照水平很低（星星很微弱，明亮的荧光粒子会毒害细胞），观察可能会受到时间和空间变化的扭曲（由于天文学中的大气，由于活细胞成像中的标本本身），根据应用，可能需要使用不同的光谱（颜色）区域或光谱带。该计划旨在利用为天文学开发的波前传感技术来解决生物学中三维成像和时变过程的问题。这些技术的成功应用将使许多活体标本的发育研究受益。