Controlled Spatiotemporal Delivery of miRNA Anatgomir for Promoting Vascular Self
受控时空递送 miRNA Anatgomir 以促进血管自身
基本信息
- 批准号:8138278
- 负责人:
- 金额:$ 20.69万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-08-19 至 2013-07-31
- 项目状态:已结题
- 来源:
- 关键词:Angiogenic ProteinsBiochemicalBiologyBiomedical EngineeringBlood VesselsBurn injuryCartilageCell Culture TechniquesCell SurvivalCellsCholesterolCollagenCytoplasmDevelopmentDiabetes MellitusDiseaseEffectivenessEncapsulatedEndothelial CellsExtravasationFeedbackFibronectinsFlow CytometryFluorescence MicroscopyFunctional RNAGelGenerationsGenesHistocompatibility TestingHumanImmunodeficient MouseImmunologyImplantIn VitroIncubatedLabelLeadLifeLiver FailureMessenger RNAMicroRNAsMicroscopyMicrospheresMonocyte Chemoattractant Protein-1Pathway interactionsPerfusionPericytesPolymersProcessProteinsPublic HealthRNA SequencesRegenerative MedicineResearch PersonnelSCID MiceSkinSmooth Muscle MyocytesStructureSuspension substanceSuspensionsSystemTechniquesTechnologyTestingTherapeuticTissue EngineeringTissuesToxic effectTranslationsVascular Endothelial Growth Factor AVascular Endothelial Growth FactorsVascularizationWorkabstractingangiogenesisbiodegradable polymerclinical practicecontrolled releasedesignimplantationimprovedin vitro Assayin vivonanoparticlenew technologyself assemblyspatiotemporaluptake
项目摘要
DESCRIPTION (provided by applicant):
Previous work has demonstrated that well differentiated human endothelial cells (ECs) will self assemble into vascular conduits in protein gels both in vitro and in vivo after implantation into immunodeficient mouse hosts. Vessel maturation in grafts containing only EC requires recruitment of host mural cells, such as vascular smooth muscle cells or pericytes (PCs). The maturation of vessels is accelerated and enhanced when ECs are co-implanted with human PCs. Vessel self assembly can also be enhanced by sustained delivery of pro- angiogenic proteins that act on ECs or PCs, especially when an EC-directed agent, vascular endothelial growth factor (VEGF), is combined with a PC-directed agent, monocyte chemotactic protein -1 (MCP-1). However, vessel self-assembly and maturation still appears too slow to optimize parenchymal cell survival, requiring at least 10 days. The actions of pro-angiogenic proteins may be augmented or limited by positive and negative feedback loops, respectively, within the target cells that involve microRNAs (miRNAs). miRNAs are short, non-coding RNAs that regulate a variety of development processes by reducing specific mRNA half lives or translation. A single miRNA can reduce the expression of multiple genes often in the same pathway. The effects of miRNAs can be inhibited by complementary short RNA sequences referred to as antagomirs. Antagomirs act in a cell-specific manner when the miRNA is expressed in a cell specific manner. This project tests the hypothesis that controlled delivery of an antagomir can enhance the therapeutic benefits of angiogenic proteins such as VEGF in vascular self-assembly. This hypothesis will be tested through two specific aims. In Aim 1, polymer nanoparticles (NP) will be used to find the optimal approaches for providing spatial and temporal control over miRNA and antagomir delivery to the cytoplasm of ECs in 3D culture. In Aim 2, these NP delivery systems will be tested for their ability to control the spatial and temporal delivery of antagomirs to miR-17/20-which is known to augment the effects of VEGF-to 3D cell cultures produced by suspending ECs and PCs in gels of collagen and fibronectin. (End of Abstract)
描述(由申请人提供):
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
JORDAN S POBER其他文献
JORDAN S POBER的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('JORDAN S POBER', 18)}}的其他基金
Ex Vivo Nanoparticle Drug Delivery Targeted to Human Allograft Endothelium
针对人同种异体移植物内皮的体外纳米颗粒药物输送
- 批准号:
10783379 - 财政年份:2023
- 资助金额:
$ 20.69万 - 项目类别:
Assessment of immunogenicity and antigenicity of different human cell types in natural and 3D-printed allografts
评估天然和 3D 打印同种异体移植物中不同人类细胞类型的免疫原性和抗原性
- 批准号:
10353416 - 财政年份:2021
- 资助金额:
$ 20.69万 - 项目类别:
Assessment of immunogenicity and antigenicity of different human cell types in natural and 3D-printed allografts
评估天然和 3D 打印同种异体移植物中不同人类细胞类型的免疫原性和抗原性
- 批准号:
10194232 - 财政年份:2021
- 资助金额:
$ 20.69万 - 项目类别:
Ex Vivo Nanoparticle Drug Delivery Targeted to Human Renal Allograft Endothelium
针对人肾同种异体移植物内皮的体外纳米颗粒药物输送
- 批准号:
10197784 - 财政年份:2017
- 资助金额:
$ 20.69万 - 项目类别:
Ex Vivo Nanoparticle Drug Delivery Targeted to Human Renal Allograft Endothelium
针对人肾同种异体移植物内皮的体外纳米颗粒药物输送
- 批准号:
10155842 - 财政年份:2017
- 资助金额:
$ 20.69万 - 项目类别:
Optimizing Therapeutic Revascularization by Endothelial Cell Transplantation
通过内皮细胞移植优化治疗性血运重建
- 批准号:
9516109 - 财政年份:2017
- 资助金额:
$ 20.69万 - 项目类别:
Targeting Nanoparticles for Drug Delivery to Renal Graft Endothelium during Ex Vivo Normothermic Perfusion
体外常温灌注期间靶向纳米颗粒将药物递送至肾移植物内皮
- 批准号:
9164300 - 财政年份:2016
- 资助金额:
$ 20.69万 - 项目类别:
Bioengineered siRNA/Nanoparticles to Prevent Human Transplant Rejection
生物工程 siRNA/纳米颗粒可防止人体移植排斥
- 批准号:
8693080 - 财政年份:2013
- 资助金额:
$ 20.69万 - 项目类别:
Spatiotemporal Delivery of miRNA Anatgomir for Promoting Vascular Self-Assembly
miRNA Anatgomir 的时空传递促进血管自组装
- 批准号:
8322816 - 财政年份:2011
- 资助金额:
$ 20.69万 - 项目类别:
相似海外基金
Elucidating the molecular basis and expanding the biological applications of the glycosyltransferases using biochemical and structural biology approaches
利用生化和结构生物学方法阐明糖基转移酶的分子基础并扩展其生物学应用
- 批准号:
23K14138 - 财政年份:2023
- 资助金额:
$ 20.69万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Utilizing the power of synthetic biology and De Novo design for the overexpression and biochemical stabilization of KCNA6 or Kv1.6 potassium channels in the E. coli expression system
利用合成生物学和 De Novo 设计的力量,实现大肠杆菌表达系统中 KCNA6 或 Kv1.6 钾通道的过度表达和生化稳定
- 批准号:
10666856 - 财政年份:2023
- 资助金额:
$ 20.69万 - 项目类别:
Developing synthetic chemical biology strategies for biochemical investigations and biomedical applications
开发用于生化研究和生物医学应用的合成化学生物学策略
- 批准号:
10623497 - 财政年份:2023
- 资助金额:
$ 20.69万 - 项目类别:
NSF Postdoctoral Fellowship in Biology FY 2022: Defining the biochemical mechanisms of microtubule shrinking and nucleation by combining innovative experiments and simulations
2022 财年 NSF 生物学博士后奖学金:通过结合创新实验和模拟来定义微管收缩和成核的生化机制
- 批准号:
2209298 - 财政年份:2022
- 资助金额:
$ 20.69万 - 项目类别:
Fellowship Award
An Integrated Biochemical and Structural Approach to Delineating the Biology of EWSR1
描述 EWSR1 生物学的综合生化和结构方法
- 批准号:
10665058 - 财政年份:2021
- 资助金额:
$ 20.69万 - 项目类别:
An Integrated Biochemical and Structural Approach to Delineating the Biology of EWSR1 - Multi-Mode Detection and Imaging of Biomolecular Condensates.
描述 EWSR1 生物学的综合生化和结构方法 - 生物分子凝聚物的多模式检测和成像。
- 批准号:
10797858 - 财政年份:2021
- 资助金额:
$ 20.69万 - 项目类别:
An Integrated Biochemical and Structural Approach to Delineating the Biology of EWSR1
描述 EWSR1 生物学的综合生化和结构方法
- 批准号:
10298663 - 财政年份:2021
- 资助金额:
$ 20.69万 - 项目类别:
Physiological and biochemical insights into the biology of marine vertebrates and the ecosystems within which they function
对海洋脊椎动物及其功能的生态系统的生物学的生理和生化见解
- 批准号:
RGPIN-2015-04374 - 财政年份:2019
- 资助金额:
$ 20.69万 - 项目类别:
Discovery Grants Program - Individual
Physiological and biochemical insights into the biology of marine vertebrates and the ecosystems within which they function
对海洋脊椎动物及其功能的生态系统的生物学的生理和生化见解
- 批准号:
RGPIN-2015-04374 - 财政年份:2018
- 资助金额:
$ 20.69万 - 项目类别:
Discovery Grants Program - Individual
Physiological and biochemical insights into the biology of marine vertebrates and the ecosystems within which they function
对海洋脊椎动物及其功能的生态系统的生物学的生理和生化见解
- 批准号:
RGPIN-2015-04374 - 财政年份:2017
- 资助金额:
$ 20.69万 - 项目类别:
Discovery Grants Program - Individual